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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
16/01/2018 |
Data da última atualização: |
16/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CARDOSO, P. C. B.; BRONDANI, C.; MENEZES, I. P. P.; VALDISSER, P. A. M. R.; BORBA, T. C. O.; DEL PELOSO, M. J.; VIANELLO, R. P. |
Afiliação: |
P. C. B. CARDOSO; CLAUDIO BRONDANI, CNPAF; I. P. P. MENEZES; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
Título: |
Discrimination of common bean cultivars using multiplexed microsatellite markers. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 13, n. 1, p. 1964-1978, 2014. |
DOI: |
10.4238/2014.March.24.1 |
Idioma: |
Inglês |
Conteúdo: |
Analysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized. Based on an analysis of 24 simple sequence repeats, the estimates for the average number of alleles and genetic diversity were 8.29 and 0.646, respectively. The combined probability of identity was estimated at 7.05 x 10(-17), indicating a high individual discriminatory power. Thirty-two percent of the cultivars exhibited heterogeneity for multiple loci that reflected either homozygosity for different alleles of a given locus in different individuals or heterozygosity for the locus. The average genetic diversity for the groups of cultivars and genitors was 0.605 and 0.660, respectively, with no genetic differentiation (F-ST) between these groups. Although similar estimates of expected heterozygosity were observed when the cultivars were grouped by release date, a greater number of private alleles was observed in the most recent cultivars. The genetic differentiation among cultivars originating from different institutions was not different from zero (F-ST = 0.01). The molecular profile database derived from these analyses may increase the statistical power of genetic estimates and may be incorporated into breeding programs for common bean. Furthermore, the profiles obtained for the different cultivars may be used as molecular descriptors to complement traditional descriptors used in distinctiveness, uniformity and stability tests, thereby improving the traceability of samples and their derivatives and helping to protect the intellectual property rights of breeders. MenosAnalysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized. Based on an analysis of 24 simple sequence repeats, the estimates for the average number of alleles and genetic diversity were 8.29 and 0.646, respectively. The combined probability of identity was estimated at 7.05 x 10(-17), indicating a high individual discriminatory power. Thirty-two percent of the cultivars exhibited heterogeneity for multiple loci that reflected either homozygosity for different alleles of a given locus in different individuals or heterozygosity for the locus. The average genetic diversity for the groups of cultivars and genitors was 0.605 and 0.660, respectively, with no genetic differentiation (F-ST) between these groups. Although similar estimates of expected heterozygosity were observed when the cultivars were grouped by release date, a greater number of private alleles was observed in the most recent cultivars. The genetic differentiation among cultivars originating from different institutions was not dif... Mostrar Tudo |
Palavras-Chave: |
Diversidade genética. |
Thesagro: |
DNA; Feijão; Marcador molecular; Phaseolus vulgaris. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171156/1/CNPAF-2014-gmr.pdf
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Marc: |
LEADER 02835naa a2200265 a 4500 001 2085520 005 2018-01-16 008 2014 bl uuuu u00u1 u #d 024 7 $a10.4238/2014.March.24.1$2DOI 100 1 $aCARDOSO, P. C. B. 245 $aDiscrimination of common bean cultivars using multiplexed microsatellite markers.$h[electronic resource] 260 $c2014 520 $aAnalysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized. Based on an analysis of 24 simple sequence repeats, the estimates for the average number of alleles and genetic diversity were 8.29 and 0.646, respectively. The combined probability of identity was estimated at 7.05 x 10(-17), indicating a high individual discriminatory power. Thirty-two percent of the cultivars exhibited heterogeneity for multiple loci that reflected either homozygosity for different alleles of a given locus in different individuals or heterozygosity for the locus. The average genetic diversity for the groups of cultivars and genitors was 0.605 and 0.660, respectively, with no genetic differentiation (F-ST) between these groups. Although similar estimates of expected heterozygosity were observed when the cultivars were grouped by release date, a greater number of private alleles was observed in the most recent cultivars. The genetic differentiation among cultivars originating from different institutions was not different from zero (F-ST = 0.01). The molecular profile database derived from these analyses may increase the statistical power of genetic estimates and may be incorporated into breeding programs for common bean. Furthermore, the profiles obtained for the different cultivars may be used as molecular descriptors to complement traditional descriptors used in distinctiveness, uniformity and stability tests, thereby improving the traceability of samples and their derivatives and helping to protect the intellectual property rights of breeders. 650 $aDNA 650 $aFeijão 650 $aMarcador molecular 650 $aPhaseolus vulgaris 653 $aDiversidade genética 700 1 $aBRONDANI, C. 700 1 $aMENEZES, I. P. P. 700 1 $aVALDISSER, P. A. M. R. 700 1 $aBORBA, T. C. O. 700 1 $aDEL PELOSO, M. J. 700 1 $aVIANELLO, R. P. 773 $tGenetics and Molecular Research$gv. 13, n. 1, p. 1964-1978, 2014.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
15/04/2008 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
BRESSAN, W.; FIGUEIREDO, J. E. F. |
Afiliação: |
WELLINGTON BRESSAN, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
Título: |
Efficacy and dose-response relationship in biocontrol of fusarium disease in maize by Streptomyces spp. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
European Journal of Plant Pathology, Dordrecht, v. 120, n. 3, p. 311-316, 2008. |
DOI: |
10.1007/s10658-007-9220-y |
Idioma: |
Inglês |
Conteúdo: |
Two isolates of Streptomyces spp. DAUFPE 11470 and DAUFPE 14632 were evaluated to determine the antagonist pathogen inoculum concentration relationship under greenhouse conditions. Pathogen and antagonist concentration, significantly (P?0.05) affected development of Fusarium disease in maize with a significant interaction between pathogen and antagonist concentration. Dose response relationship also differed significantly (P?0.05) between the two isolates, but both isolates demonstrated effective control of Fusarium disease, regardless of pathogen concentration. The isolate DAUFPE 11470 provided the most effective control. The lowest value for disease incidence occurred at low pathogen (103 chlamydospore g-1 soil) and high antagonist concentration (106 cfu ml-1) for both isolates. The disease incidence for control plants ranged from 19% to 76%. However, in relation to control the lowest disease reduction occurred at low pathogen (103 chlamydospore g-1 soil) and high antagonist concentrations (106 cfu ml-1). These reductions were 10.6% and 13% for DAUFPE 14632 and DAUFPE 11470, respectively. The highest disease reductions, in relation to control plants, occurred at high pathogen (106 chlamydospore g-1 soil) and antagonist (106 cfu ml-1) concentrations for both isolates. These values were 55% and 62.2% for DAUFPE 14632 and DAUFPE 11470, respectively. The chlamydospore germination of Fusarium moniliforme was affected by glucose addition, antagonist isolates and type of inoculum. The lowest chlamydospore germination was observed with bacterial suspensions of the isolates, for all glucose additions. The results suggested that both Streptomyces spp. isolates were effective at different doses as biocontrol agents against F. moniliforme. Also, there was evidence for at least two mechanisms of biocontrol and that apparently, both isolates showed the same mechanisms of biocontrol action related to production of bioactive compounds and competition for carbon. Further studies will be developed to improve the level and effectiveness of control by these isolates. MenosTwo isolates of Streptomyces spp. DAUFPE 11470 and DAUFPE 14632 were evaluated to determine the antagonist pathogen inoculum concentration relationship under greenhouse conditions. Pathogen and antagonist concentration, significantly (P?0.05) affected development of Fusarium disease in maize with a significant interaction between pathogen and antagonist concentration. Dose response relationship also differed significantly (P?0.05) between the two isolates, but both isolates demonstrated effective control of Fusarium disease, regardless of pathogen concentration. The isolate DAUFPE 11470 provided the most effective control. The lowest value for disease incidence occurred at low pathogen (103 chlamydospore g-1 soil) and high antagonist concentration (106 cfu ml-1) for both isolates. The disease incidence for control plants ranged from 19% to 76%. However, in relation to control the lowest disease reduction occurred at low pathogen (103 chlamydospore g-1 soil) and high antagonist concentrations (106 cfu ml-1). These reductions were 10.6% and 13% for DAUFPE 14632 and DAUFPE 11470, respectively. The highest disease reductions, in relation to control plants, occurred at high pathogen (106 chlamydospore g-1 soil) and antagonist (106 cfu ml-1) concentrations for both isolates. These values were 55% and 62.2% for DAUFPE 14632 and DAUFPE 11470, respectively. The chlamydospore germination of Fusarium moniliforme was affected by glucose addition, antagonist isolates and type of inoc... Mostrar Tudo |
Palavras-Chave: |
Maize; Seedborne diseases. |
Thesagro: |
Fusarium Moniliforme; Milho. |
Thesaurus NAL: |
Streptomyces. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02749naa a2200205 a 4500 001 1484608 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 024 7 $a10.1007/s10658-007-9220-y$2DOI 100 1 $aBRESSAN, W. 245 $aEfficacy and dose-response relationship in biocontrol of fusarium disease in maize by Streptomyces spp.$h[electronic resource] 260 $c2008 520 $aTwo isolates of Streptomyces spp. DAUFPE 11470 and DAUFPE 14632 were evaluated to determine the antagonist pathogen inoculum concentration relationship under greenhouse conditions. Pathogen and antagonist concentration, significantly (P?<?0.05) affected development of Fusarium disease in maize with a significant interaction between pathogen and antagonist concentration. Dose response relationship also differed significantly (P?<?0.05) between the two isolates, but both isolates demonstrated effective control of Fusarium disease, regardless of pathogen concentration. The isolate DAUFPE 11470 provided the most effective control. The lowest value for disease incidence occurred at low pathogen (103 chlamydospore g-1 soil) and high antagonist concentration (106 cfu ml-1) for both isolates. The disease incidence for control plants ranged from 19% to 76%. However, in relation to control the lowest disease reduction occurred at low pathogen (103 chlamydospore g-1 soil) and high antagonist concentrations (106 cfu ml-1). These reductions were 10.6% and 13% for DAUFPE 14632 and DAUFPE 11470, respectively. The highest disease reductions, in relation to control plants, occurred at high pathogen (106 chlamydospore g-1 soil) and antagonist (106 cfu ml-1) concentrations for both isolates. These values were 55% and 62.2% for DAUFPE 14632 and DAUFPE 11470, respectively. The chlamydospore germination of Fusarium moniliforme was affected by glucose addition, antagonist isolates and type of inoculum. The lowest chlamydospore germination was observed with bacterial suspensions of the isolates, for all glucose additions. The results suggested that both Streptomyces spp. isolates were effective at different doses as biocontrol agents against F. moniliforme. Also, there was evidence for at least two mechanisms of biocontrol and that apparently, both isolates showed the same mechanisms of biocontrol action related to production of bioactive compounds and competition for carbon. Further studies will be developed to improve the level and effectiveness of control by these isolates. 650 $aStreptomyces 650 $aFusarium Moniliforme 650 $aMilho 653 $aMaize 653 $aSeedborne diseases 700 1 $aFIGUEIREDO, J. E. F. 773 $tEuropean Journal of Plant Pathology, Dordrecht$gv. 120, n. 3, p. 311-316, 2008.
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