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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
17/06/2008 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOMFETI, C. A.; SOUZA-PACCOLA, E. A.; MASSOLA JUNIOR, N. S.; MARRIEL, I. E.; MEIRELLES, W. F.; CASELA, C. R.; PACCOLA-MEIRELLES, L. D. |
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Afiliação: |
Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL. |
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Título: |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008. |
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DOI: |
10.1590/S1982-56762008000100010 |
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Idioma: |
Inglês |
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Conteúdo: |
The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. MenosThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. T... Mostrar Tudo |
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Palavras-Chave: |
Doença bacteriana do milho; doenças foliares do milho; Maize bacterial disease; Maize leaf disease. |
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Thesagro: |
Milho; Zea Mays. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32277/1/Localization-pantoea.pdf
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Marc: |
LEADER 02604naa a2200277 a 4500
001 1491172
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008 2008 bl uuuu u00u1 u #d
024 7 $a10.1590/S1982-56762008000100010$2DOI
100 1 $aBOMFETI, C. A.
245 $aLocalization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.$h[electronic resource]
260 $c2008
520 $aThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis.
650 $aMilho
650 $aZea Mays
653 $aDoença bacteriana do milho
653 $adoenças foliares do milho
653 $aMaize bacterial disease
653 $aMaize leaf disease
700 1 $aSOUZA-PACCOLA, E. A.
700 1 $aMASSOLA JUNIOR, N. S.
700 1 $aMARRIEL, I. E.
700 1 $aMEIRELLES, W. F.
700 1 $aCASELA, C. R.
700 1 $aPACCOLA-MEIRELLES, L. D.
773 $tTropical Plant Pathology, Brasília$gv. 33, n. 1, p. 63-66, Jan./Feb. 2008.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 11 | |
| 1. |  | BOMFETI, C. A.; MEIRELLES, W. F.; SOUZA-PACCOLA, E.A.; CASELA, C. R.; FERREIRA, A. S.; PACCOLA-MEIRELLES, L.D. Avaliacao de agroquímicos comerciais (in vitro e in vivo) no controle da doença descrita como mancha foliar de Phaeosphaeria em milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 25.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 1., 2004, Cuiabá, MT. Da agricultura familiar ao agronegócio: tecnologia, competitividade e sustentabilidade: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Cuiabá: Empaer, 2004. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|    |
| 3. | | FAVARO, L. C. L.; SOUZA-PACCOLA, E,A,; BOMFETI, C. A.; CASELA, C. R.; PACCOLA-MEIRELLES, L.D. Indução de alterações genéticas em isolados de Colletotrichum graminicola auxiliares no esclarecimento(s) processo(s) que gera(m) alta variabilidade patogênica. In: CONGRESSO NACIONAL DE MILHO E SORGO, 24., 2002, Florianópolis, SC. Meio ambiente e a nova agenda para o agronegócio de milho e sorgo: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Florianópolis: Epagri, 2002.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 4. | | BOMFETI, C. A.; MEIRELLES, W. F.; SOUZA-PACCOLA, E.A.; CASELA, C. R.; PACCOLA-MEIRELLES, L.D. Reação de cultivares de milho a mancha foliar de Phaeosphaeria avaliada através da severidade da doença e do padrão morfológico das lesões. In: CONGRESSO NACIONAL DE MILHO E SORGO, 24., 2002, Florianópolis, SC. Meio ambiente e a nova agenda para o agronegócio de milho e sorgo: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Florianópolis: Epagri, 2002.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | OLIVEIRA-LONGATTI, S. M. de; MARRA, L. M.; SOARES, B. L.; BOMFETI, C. A.; SILVA, K. da; FERREIRA, P. A. A.; MOREIRA, F. M. de S. Bacteria isolated from soils of the western Amazon and from rehabilitated bauxite-mining areas have potential as plant growth promoters. World Journal of Microbiology & Biotechnology, v. 30, p.1239-1250, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Roraima. |
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| 6. | | BOMFETI, C. A.; SOUZA-PACCOLA, E. A.; MASSOLA JUNIOR, N. S.; MARRIEL, I. E.; MEIRELLES, W. F.; CASELA, C. R.; PACCOLA-MEIRELLES, L. D. Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | BOMFETI, C. A.; MEIRELLES, W. F.; SOUZA-PACCOLA, E. A.; CASELA, C. R.; FERREIRA, A. da S.; MARRIEL, I. E.; PACCOLA-MEIRELLES, L. D. Avaliação de produtos químicos comerciais, in vitro e in vivo, no controle da doença foliar, mancha branca do milho, causada por Pantoea ananatis. Summa Phytopathologica, Botucatu, v. 33, n. 1, p. 63-67, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | FERREIRA, P. A. A.; BOMFETI, C. A.; SILVA JÚNIOR, R. da; SOARES, B. L.; SOARES, C. R. F. S.; MOREIRA, F. M. de S. Eficiência simbiótica de estirpes de Cupriavidus necator tolerantes a zinco, cádmio, cobre e chumbo. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 1, p. 85-95, jan. 2012. Título em inglês: Symbiotic efficiency of Cupriavidus necator strains tolerant to zinc, cadmium, copper and lead.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | SOUZA-PACCOLA, E. A de; BOMFETI, C. A.; TANAKA, F. A. O.; MASSOLA JUNIOR, N. S.; COLAUTO, N. B.; FIGUEIREDO, J. E. F.; PACCOLA-MEIRELLES, L. D. Novel insights into the early stages of infection by oval conidia of Colletotrichum sublineolum. Scientia Agricola, Piracicaba, v. 72, n. 4, p. 351-355, July/Aug. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 10. | | PACCOLA-MEIRELLES, L.D.; BOMFETI, C. A.; SOUZA-PACCOLA, E.A.; MARRIEL, I. E.; MESQUITA, S. F. P.; MASSOLA JUNIOR, N.; CASELA, C. R.; MEIRELLES, W. F.; FERREIRA, A. S. Confirmação da etiologia da doença descrita na Brasil como sendo a mancha foliar de Phaeosphaeria em milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 25.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 1., 2004, Cuiabá, MT. Da agricultura familiar ao agronegócio: tecnologia, competitividade e sustentabilidade: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Cuiabá: Empaer, 2004. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 11. | | MARRIEL, I. E.; FERREIRA, A. S.; CASELA, C. R.; UTIDA, M. K.; MONTEIRO, G. G; CARNEIRO, A. A.; BOMFETI, C. A.; SOUZA-PACCOLA, E.A.; PACCOLA-MEIRELLES, L.D. Perfil metabólico de bactérias isoladas de lesões de manchas foliar de Phaeosphaeria em milho, através do sistema Biolog. In: CONGRESSO NACIONAL DE MILHO E SORGO, 25.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 1., 2004, Cuiabá, MT. Da agricultura familiar ao agronegócio: tecnologia, competitividade e sustentabilidade: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Cuiabá: Empaer, 2004. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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