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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
27/10/1998 |
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Data da última atualização: |
27/10/1998 |
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Autoria: |
XIA, X.; BOLLINGER, J.; OGRAM, A. |
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Título: |
Molecular genetic analysis of the response of three soil microbial communities to the application of 2,4-D |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Molecular Ecology, v.4, p.17-28, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
The responses of three different soil microbial communities to the experimental application of 2,4-dichlorophenoxyacetic acid(2,4-D) were evaluated with a variety of molecular genetic techniques. Two of the three soil communities had histories of prior direct exposure to 2,4-D, and one had no prior direct application of any herbicide. Dominant 2,4-D degrading strains isolated from these soils the previous year were screened for hybridization with three catabolic genes (tfdA, tfdAII, and tfdB) cloned from the wellstudied 2,4-D degradative plasmid, pJP4, revealing varying degrees of similarity with the three genes. Hybridization of total community DNA from the three of similarity with the three genes. |
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Palavras-Chave: |
Impressao digital; Microbiologia de solo; Pingerprint; Random amplified polymorphic; RAPD; Soil microbiology. |
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Thesagro: |
DNA. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01346naa a2200229 a 4500
001 1366709
005 1998-10-27
008 1995 bl --- 0-- u #d
100 1 $aXIA, X.
245 $aMolecular genetic analysis of the response of three soil microbial communities to the application of 2,4-D
260 $c1995
520 $aThe responses of three different soil microbial communities to the experimental application of 2,4-dichlorophenoxyacetic acid(2,4-D) were evaluated with a variety of molecular genetic techniques. Two of the three soil communities had histories of prior direct exposure to 2,4-D, and one had no prior direct application of any herbicide. Dominant 2,4-D degrading strains isolated from these soils the previous year were screened for hybridization with three catabolic genes (tfdA, tfdAII, and tfdB) cloned from the wellstudied 2,4-D degradative plasmid, pJP4, revealing varying degrees of similarity with the three genes. Hybridization of total community DNA from the three of similarity with the three genes.
650 $aDNA
653 $aImpressao digital
653 $aMicrobiologia de solo
653 $aPingerprint
653 $aRandom amplified polymorphic
653 $aRAPD
653 $aSoil microbiology
700 1 $aBOLLINGER, J.
700 1 $aOGRAM, A.
773 $tMolecular Ecology$gv.4, p.17-28, 1995.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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