Data/hora: 18/06/2024 01:20:34

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 10/03/2016
Data da última atualização: | 10/03/2016
Autoria: | SANTOS, F. C.; SILVA, J. F.; BOELONI, J. N.; TEIXEIRA, E.; TURRA, E. M.; SERAKIDES, R.; OCARINO, N. M.
Afiliação: | FERNANDA C. SANTOS, EV/UFMG; JUNEO F. SILVA, EV/UFMG; JANKERLE N. BOELONI, UV/UFMG; EDGAR TEIXEIRA, EV/UFMG; EDUARDO M. TURRA, UV/UFMG; ROGÉRIA SERAKIDES, EV/UFMG; NATÁLIA M. OCARINO, EV/UFMG.
Título: | Morphological and immunohistochemical characterization of angiogenic and apoptotic factors and the expression of thyroid receptors in the ovary of tilapia Oreochromis niloticus in captivity.
Ano de publicação: | 2015
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Brasília, DF, v. 35. n. 4, p. 371-376, abr. 2015.
Idioma: | Inglês
Conteúdo: | Morphological and immunohistochemical characterization of angiogenic and apoptotic factors and the expression of thyroid receptors in the ovary of tilapia Oreochromis niloticus in captivity were studied. The morphological evaluation of the ovaries was performed by histological paraffin embedded and stained with HE. The immunohistochemical expressions of CDC47, VEGF, Flk-1, angiopoietin, Tie-2 and thyroid receptor (TR&#945;) were performed by the technique of streptavidein-biotin-peroxidase. Apoptosis was assessed using the TUNEL kit. The relative expression of thyroid hormone receptors (TR&#945; and TR&#946;) was assessed by RT-PCR real time. The nuclear expression of CDC47 increased with the stage of maturation of the oocyte and was observed in the follicle cells. Apoptotic bodies were observed in the follicular cells of atretic follicles and postovulatory follicles from the ovaries of 150g and 350g fish. Expression of VEGF and its receptor Flk-1 was also observed in the follicular cells, and the expression of both increased with the maturity of the oocyte, with a higher intensity observed in the full-grown follicle. The expression of angiopoietin and of its receptor (Tie 2) was discrete and moderate respectively. TR&#945; expression was independent of follicular development. However, the 350 g tilapia exhibited higher expression of TR&#946; compared with the 50 g tilapia. We conclude that the proliferative activity and the expression of VEGF and its receptor increase with follicular maturation and that the TRs expression increases with ovarian maturity in tilapia (Oreochromis niloticus).
Palavras-Chave: | Angiogenic factor; Apoptotic factor; Fator angiogênico; Fator apoptótico; Imuno-histoquímica; Receptor tireoidiano; Thyroid receptors.
Thesagro: | Oreochromis Niloticus; Ovário; Peixe; Tilápia.
Thesaurus Nal: | Fish; Immunohistochemistry.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/140987/1/Morphological-and-immunohistochemical.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59102-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS