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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
05/03/2012 |
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Data da última atualização: |
19/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; RECHENMACHER, C.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, M. S.; DEL PONTE, E. M.; CARLINI, C. R.; CARVALHO, M. C. C. G. de; BODANESE-ZANETTINI, M. H. |
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Afiliação: |
BEATRIZ WIEBKE-STROHM, UFRGS; GIANCARLO PASQUALI, UFRGS; MÁRCIA MARGIS-PINHEIRO, UFRGS; MARTA BENCKE, UFRGS; LAURO BÜCKER NETO, UFRGS; ARLETE B. BECKER-RITT, UFRGS; ANNE H. S. MARTINELLI, UFRGS; CILIANA RECHENMACHER, UFRGS; JOSEPH C. POLACCO, University of Missouri; RENATA STOLF, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; MILENA S. HOMRICH, UFRGS; EMERSON M. DEL PONTE, UFRGS; CELIA R. CARLINI, UFRGS; MAYRA C. C. G. DE CARVALHO, CNPSo; MARIA HELENA BODANESE-ZANETTINI, UFRGS. |
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Título: |
Ubiquitous urease affects soybean susceptibility to fungi. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Plant Molecular Biology, The Hague, v. 79, n. 1/2, p. 75-87, May 2012. |
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DOI: |
DOI 10.1007/s11103-012-9894-1 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach. MenosThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of u... Mostrar Tudo |
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Palavras-Chave: |
Penicillium herguei; Transformação genética. |
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Thesagro: |
Fungo; Phomopsis; Queima da saia; Soja. |
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Thesaurus Nal: |
Genetic transformation; Soybeans. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02843naa a2200421 a 4500
001 1917463
005 2017-01-19
008 2012 bl uuuu u00u1 u #d
024 7 $aDOI 10.1007/s11103-012-9894-1$2DOI
100 1 $aWIEBKE-STROHM, B.
245 $aUbiquitous urease affects soybean susceptibility to fungi.
260 $c2012
520 $aThe soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from nontransgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach.
650 $aGenetic transformation
650 $aSoybeans
650 $aFungo
650 $aPhomopsis
650 $aQueima da saia
650 $aSoja
653 $aPenicillium herguei
653 $aTransformação genética
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aBENCKE, M.
700 1 $aBÜCKER-NETO, L.
700 1 $aBECKER-RITT, A. B.
700 1 $aMARTINELLI, A. H. S.
700 1 $aRECHENMACHER, C.
700 1 $aPOLACCO, J. C.
700 1 $aSTOLF, R.
700 1 $aMARCELINO, F. C.
700 1 $aABDELNOOR, R. V.
700 1 $aHOMRICH, M. S.
700 1 $aDEL PONTE, E. M.
700 1 $aCARLINI, C. R.
700 1 $aCARVALHO, M. C. C. G. de
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tPlant Molecular Biology, The Hague$gv. 79, n. 1/2, p. 75-87, May 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 32 | |
| 21. |  | HOMRICH, M. S.; PASSAGLIA, L. M. P.; PEREIRA, J. F.; BERTAGNOLLI, P. F.; PASQUALI, G.; ZAIDI, M. A.; ALTOSAAR, I.; BODANESE-ZANETTINI, M. H. Resistance to Anticarsia gemmatalis Hübner (Lepidoptera, Noctuidae) in transgenic soybean (Glycine max L.) Merrill Fabales, Fabaceae) cultivar IAS5 expressing a modified Cry1 Ac endotoxin. Genetics and Molecular Biology, v. 31, n. 2, p. 522-531, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Trigo. |
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| 22. | | DROSTE, A.; SILVA, A. M. da; SOUZA, I. F. de; WIEBKE-STROHM, B.; BÜCKER-NETO, L.; SAUNER, M. V.; BODANESE-ZANETTINI, M. H. Screening of Brazilian soybean genotypes with high potential for somatic embryogenesis and plant regeneration. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 7, p. 715-720, jul. 2010 Título em português: Seleção de genótipos brasileiros de soja com alto potencial para embriogênese somática e regeneração de plantas.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 23. | | RECHENMACHER, C.; KAPPAUN, K.; WIEBKE-STROHM, B.; BUSATTO, L. A. de O.; CORSO, M. C. M.; MARCELINO-GUIMARÃES, F. C.; CARLINI, C. R.; BODANESE-ZANETTINI, M. H. Soyuretox, a new peptide to confer partial resistance to soybean root-knot nematodes. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstract... Abstract: 664.pdf.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 24. | | RECHENMACHER, C.; WIEBKE-STROHM, B.; OLIVEIRA-BUSATTO, L. A. de; WEBER, R. L. M.; CORSO, M. C. M.; LOPES-CAITAR, V. S.; SILVA, S. M. H; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C.; CARLINI, C. R.; BODANESE-ZANETTINI, M. H. Endogenous soybean peptide overexpression: an alternative to protect plants against root-knot nematodes Biotechnology Research & Innovation, v. 3, p. 10-18, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Soja. |
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| 25. | | CABREIRA, C.; CAGLIARI, A.; BÜCKER-NETO, L.; WIEBKE-STROHM, B.; FREITAS, L. B. de; MARCELINO-GUIMARÃES, F. C.; NEPOMUCENO, A. L.; MARGIS-PINHEIRO, M. M. A. N.; BODANESE-ZANETTINI, M. H. The lesion simulating disease (LSD) gene family as a variable in soybean response to Phakopsora pachyrhizi infection and dehydration. Functional & Integrative Genomics, v. 13, n. 3, p. 323-338, Aug. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 26. | | POERSCH-BORTOLON, L. B.; PEREIRA, J. F.; NHANI JUNIOR, A.; SOTO GONZÁLES, H. H.; TORRES, G. A. M.; CONSOLI, L.; ARENHART, R. A.; BODANESE-ZANETTINI, M. H.; MARGIS-PINHEIRO, M. Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado Biome. Genetics and Molecular Biology, Ribeirão Preto, v. 39, n. 4, p. 629-645, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Trigo. |
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| 27. | | POERSCH-BORTOLON, L. B.; PEREIRA, J. F.; NHANI JUNIOR, A.; SOTO GONZÁLES, H. H. S.; PATRICIO, D. I.; TORRES, G. A. M.; CONSOLI, L.; MARGIS-PINHEIRO, M.; BODANESE-ZANETTINI, M. H. Transcriptome analysis of wheat under drought. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 43.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 28. | | BENCKE-MALATO, M.; CABREIRA, C.; WIEBKE-STROHM, B.; BÜCKER-NETO, L.; MANCINI, E.; OSORIO, M. B.; HOMRICH, M. S.; TURCHETTO-ZOLET, A. C.; CARVALHO, M. C. C. G. de; STOLF, R.; WEBER, R. L. M.; WESTERGAARD, G.; CASTAGNARO, A. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.; MARGIS-PINHEIRO, M.; BODANESE-ZANETTINI, M. H. Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection. BMC Plant Biology, v. 14, n. 1, article 236, Sept. 2014. 18 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 29. | | WEBER, R. L. M. W.; WIEBKE-STROHM, B.; BREDEMEIER, C.; MARGIS-PINHEIRO, M.; BRITO, G. G. de; RECHENMACHER, C.; BERTAGNOLLI, P. F.; SÁ, M. E. L. de; CAMPOS, M. de A.; AMORIM, R. M. S. de; BENEVENTI, M. A.; MARGIS, R.; GROSSI-DE SA, M. F.; BODANESE-ZANETTINI, M. H. Expression of an osmotin-like protein from Solanum nigrum confers drought tolerance in transgenic soybean. BMC Plant Biology, v. 14:343, 2014. (Open Access).| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 30. | | WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, S. H.; DEL PONTE, E. M.; CARLINI, C. R.; BODANESE-ZANETTINI, M. H. Urease affects soybean susceptibility to fungi. In: BRAZILIAN SYMPOSIUM ON PLANT MOLECULAR BIOLOGY, 3., 2011, Ilhéus. [Abstracts...]. [Ribeirão Preto]: SBG, 2011. 1 p. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 31. | | WIEBKE-STROHM, B.; PASQUALI, G.; MARGIS-PINHEIRO, M.; BENCKE, M.; BÜCKER-NETO, L.; BECKER-RITT, A. B.; MARTINELLI, A. H. S.; RECHENMACHER, C.; POLACCO, J. C.; STOLF, R.; MARCELINO, F. C.; ABDELNOOR, R. V.; HOMRICH, M. S.; DEL PONTE, E. M.; CARLINI, C. R.; CARVALHO, M. C. C. G. de; BODANESE-ZANETTINI, M. H. Ubiquitous urease affects soybean susceptibility to fungi. Plant Molecular Biology, The Hague, v. 79, n. 1/2, p. 75-87, May 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 32. | | ABDELNOOR, R. V.; NEPOMUCENO, A. L.; BARROS, E. G. de; SA, M. de F. G. de; BINNECK, E.; MARCELINO, F. C.; BROMMONSCHENKEL, S. H.; ALMEIDA, J.; ISEPPON, A. M. B.; SCHUSTER, I.; KIDO, E. A.; LOUREIRO, M. E.; MARGIS, R.; HUNGRIA, M.; MOREIRA, M. A.; BACARAT-PEREIRA, M. C.; FIETTO, L. G.; BODANESE-ZANETTINI, M. H.; ROMANO, E.; ZERBINI, F. M.; LEMOS, E. G. de M.; PEREIRA, G. A. G. GENOSOJA - A brazilian soybean genome consortium. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 108, ref. O201. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 32 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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