Data/hora: 10/06/2024 04:55:25

Biblioteca(s): 	Embrapa Unidades Centrais.	
Data corrente: 	19/09/2017	
Data da última atualização: 	29/12/2017	
Autoria: 	CORRÊA, L. B. N. S.; CARDOZO, L. M. de F.; RIBEIRO, I. C. de A.; BOAVENTURA, G. T.; CHAGAS, M. A.	
Afiliação: 	Lanna B. N. S. Corrêa, Laboratório de Biomorfologia Celular e Extracelular/Departamento de Morfologia/Universidade Federal Fluminense - UFF; Ludmila F. M. de F. Cardozo, Laboratório de Nutrição Experimental - LabNE/Departamento de Nutrição/Universidade Federal Fluminense - UFF; Ilma C. de A. Ribeiro, Laboratório de Biomorfologia Celular e Extracelular/Departamento de Morfologia/Universidade Federal Fluminense - UFF; Gilson T. Boaventura, Laboratório de Nutrição Experimental - LabNE/Departamento de Nutrição/Universidade Federal Fluminense - UFF; Maurício A. Chagas, Laboratório de Biomorfologia Celular e Extracelular/Departamento de Morfologia/Universidade Federal Fluminense - UFF.	
Título: 	Influence of prolonged flaxseed (Linum usitatissimum) consumption over epididymis and testicle histoarchitecture of Wistar rats.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 650-656, jun. 2017.	
Idioma: 	Inglês	
Notas: 	Título em português: Influência do consumo prolongado de semente de linhaça (Linum usitatissimum) sobre a histoarquitetura dos testículo e epidídimo de ratos Wistar.	
Conteúdo: 	Flaxseed is considered a functional food with several health benefits. However, because of its high phytoestrogen content, flaxseed influences hormone metabolism and affects the gonadal biomorphology. In this study, computerized histomorphometry was used to evaluate seminiferous and epididymal tubules, considering the different regions of the epididymis (head, body and tail) of rats subjected to a prolonged diet of flaxseed. Young adult male Wistar rats (n=20) were divided into 2 groups during their lactation period: Control Group (CG), fed casein-based meals and Flaxseed Group (FG), fed a 25% flaxseed meal. After 250 days of continuous ingestion, the animals were euthanized and a blood sample was collected. The testicles and epididymis were removed and fixed in buffered formalin solution. The samples were subjected to routine histological paraffin techniques and stained with hematoxilin and eosin. Immunostaining was performed using an antivimentin antibody for Sertoli cell identification. For morphometry, images of the slides were scanned and analyzed using Image J to determine the epithelial height, tubular and luminal diameter and tubular and luminal area. In the hormonal evaluation, FG had a higher serum concentration of estrogen (P=0.001), but no change was observed in the concentration of testosterone. The morphometric assay of seminiferous tubules and epididymal regions revealed no significant differences between the analyzed groups. Similarly, Sertoli cell quantification showed no significant differences in the FG (P=0.98). These results revealed that the continuous and prolonged intake of 25% flaxseed meals from gestation to 250 days of age, even with a significant increase in serum levels of estradiol, does not exert adverse effects on the testicular and epididymal structure or on the cells participating in the spermatogenesis of rats.	
Palavras-Chave: 	Células de Sertoli; Fitoestrógenos; Histomorfometria; Histomorphometry; Ratos Wistar; Túbulos seminíferos; Vimentina.	
Thesagro: 	Linhaça; Linum Usitatissimum; Semente; Testículo.	
Thesaurus Nal: 	Epididymis; Linseed; Plant estrogens; Seminiferous tubules; Sertoli cells; Testes; Vimentin.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/163970/1/Influence-of-prolonged-flaxseed.pdf	
Registro original: 	Embrapa Unidades Centrais (AI-SEDE)	
Exemplares: 	AI-SEDE - (61729-1) - UPE - AP - DD -  -  -  - DIS