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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/02/2008 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOASCARIOL-CAMARGO, R. L.; BERGER, I. J.; SOUZA, A. A.; AMARAL, A. M. do; CARLOS, E. F.; FREITAS-ÁSTUA, J.; TAKITA, M. A.; TARGON, M. L. P. N.; MEDINA, C. L.; REIS, M. S.; MACHADO, M. A. |
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Afiliação: |
Raquel L. Boscariol-Camargo, APTA; Irving J. Berger, APTA; Alessandra A. Souza, APTA; Alexandre M. do Amaral, CENARGEN; Eduardo F. Carlos, APTA; Juliana Freitas-Astúa, CNPMF; Marco A. Takita, APTA; Maria Luisa P.N. Targon, APTA; Camilo L. Medina, Grupo de Consultores em Citros; Marcelo S. Reis, APTA; Marcos A. Machado, APTA. |
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Título: |
In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 906-916, 2007. |
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ISSN: |
1415-4757 |
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Idioma: |
Inglês |
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Conteúdo: |
CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and proline-related synthase. Some sequences could not be classified by using FunCat and remained with an unknown function. A large number of sequences presented high similarities to annotated genes involved with cell energy, protein synthesis and cellular transport, suggesting that Rangpur lime may sustain active cell growth under stressed condition. The presence of membrane transporters and cell signaling components could be an indication of a coordinated morphological adaptation and biochemical response during drought, helping to explain the higher tolerance of this rootstock to water stress. |
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Palavras-Chave: |
Molecular response; Rootstock; Transcription. |
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Thesaurus Nal: |
drought; expressed sequence tags. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02289naa a2200313 a 4500
001 1654648
005 2023-07-24
008 2007 bl uuuu u00u1 u #d
022 $a1415-4757
100 1 $aBOASCARIOL-CAMARGO, R. L.
245 $aIn silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress.$h[electronic resource]
260 $c2007
520 $aCitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and proline-related synthase. Some sequences could not be classified by using FunCat and remained with an unknown function. A large number of sequences presented high similarities to annotated genes involved with cell energy, protein synthesis and cellular transport, suggesting that Rangpur lime may sustain active cell growth under stressed condition. The presence of membrane transporters and cell signaling components could be an indication of a coordinated morphological adaptation and biochemical response during drought, helping to explain the higher tolerance of this rootstock to water stress.
650 $adrought
650 $aexpressed sequence tags
653 $aMolecular response
653 $aRootstock
653 $aTranscription
700 1 $aBERGER, I. J.
700 1 $aSOUZA, A. A.
700 1 $aAMARAL, A. M. do
700 1 $aCARLOS, E. F.
700 1 $aFREITAS-ÁSTUA, J.
700 1 $aTAKITA, M. A.
700 1 $aTARGON, M. L. P. N.
700 1 $aMEDINA, C. L.
700 1 $aREIS, M. S.
700 1 $aMACHADO, M. A.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 30, n. 3, p. 906-916, 2007.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | BOASCARIOL-CAMARGO, R. L.; BERGER, I. J.; SOUZA, A. A.; AMARAL, A. M. do; CARLOS, E. F.; FREITAS-ÁSTUA, J.; TAKITA, M. A.; TARGON, M. L. P. N.; MEDINA, C. L.; REIS, M. S.; MACHADO, M. A. In silico analysis of ESTs from roots of Rangpur lime (Citrus limonia Osbeck) under water stress. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 906-916, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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