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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
26/11/1999 |
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Data da última atualização: |
15/05/2023 |
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Autoria: |
MANDERS, G.; OTONI, W. C.; VAZ, F. B. d'U.; BLACKHALL, N. W.; POWER, J. B.; DAVEY, M. R. |
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Afiliação: |
Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, Nottingham NG7 2RD, UK; Swedish University of Agriculture, Department of Forest Genetics, P. O. Box 7027, S-75007 Uppsala. |
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Título: |
Transformation of passionfruit (Passiflora edulis fv flavicarpa Degener.) using Agrobacterium tumefaciens. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Plant Cell Reports, v.13, p.697-702, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
Leaf and stem explants of passiofruit (Passiflora edulis fv flavicarpa) were co-cultivated with a disarmed strain of Agrobacterium tumefaciens harbouring the co-integrate vector pMON200. Four plants of passionfruit were regenerated from leaf explants on agar-sokidified Murashige and Skoog (1962) based medium containing 4,43 uM 6-benzyl-aminopurine and supplemented with 86 uM kanamycin sulphate. The four plants were rooted by transfer to MS based medium with 14,7 uM 3-indolebutyric acid and 2.68 uM a-naphthyleneacetic acid for 7 d, followed by MS based medium lacking growth regulators. Both media used for rooting contained 172 uM kanamycin sulphate. Rooted plants were potted and grown to maturity. Three of the plants synthesised nopaline and expressed neomycin phosphotransferase activity; DNA dot blot and polymerase chain reaction analyses confirmed the presence of the neomycin phosphotransferase gene in three plants. |
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Palavras-Chave: |
Agrobacteria; Cultura de tecidos; Passionfruit; Regeneracao de apices; Shoot regeration. |
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Thesagro: |
Maracujá; Passiflora Edulis. |
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Thesaurus Nal: |
Agrobacterium; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01760naa a2200289 a 4500
001 1648427
005 2023-05-15
008 1994 bl uuuu u00u1 u #d
100 1 $aMANDERS, G.
245 $aTransformation of passionfruit (Passiflora edulis fv flavicarpa Degener.) using Agrobacterium tumefaciens.$h[electronic resource]
260 $c1994
520 $aLeaf and stem explants of passiofruit (Passiflora edulis fv flavicarpa) were co-cultivated with a disarmed strain of Agrobacterium tumefaciens harbouring the co-integrate vector pMON200. Four plants of passionfruit were regenerated from leaf explants on agar-sokidified Murashige and Skoog (1962) based medium containing 4,43 uM 6-benzyl-aminopurine and supplemented with 86 uM kanamycin sulphate. The four plants were rooted by transfer to MS based medium with 14,7 uM 3-indolebutyric acid and 2.68 uM a-naphthyleneacetic acid for 7 d, followed by MS based medium lacking growth regulators. Both media used for rooting contained 172 uM kanamycin sulphate. Rooted plants were potted and grown to maturity. Three of the plants synthesised nopaline and expressed neomycin phosphotransferase activity; DNA dot blot and polymerase chain reaction analyses confirmed the presence of the neomycin phosphotransferase gene in three plants.
650 $aAgrobacterium
650 $atissue culture
650 $aMaracujá
650 $aPassiflora Edulis
653 $aAgrobacteria
653 $aCultura de tecidos
653 $aPassionfruit
653 $aRegeneracao de apices
653 $aShoot regeration
700 1 $aOTONI, W. C.
700 1 $aVAZ, F. B. d'U.
700 1 $aBLACKHALL, N. W.
700 1 $aPOWER, J. B.
700 1 $aDAVEY, M. R.
773 $tPlant Cell Reports$gv.13, p.697-702, 1994.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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