Data/hora: 09/06/2024 19:49:16

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	26/11/1999	
Data da última atualização: 	15/05/2023	
Autoria: 	MANDERS, G.; OTONI, W. C.; VAZ, F. B. d'U.; BLACKHALL, N. W.; POWER, J. B.; DAVEY, M. R.	
Afiliação: 	Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, Nottingham NG7 2RD, UK; Swedish University of Agriculture, Department of Forest Genetics, P. O. Box 7027, S-75007 Uppsala.	
Título: 	Transformation of passionfruit (Passiflora edulis fv flavicarpa Degener.) using Agrobacterium tumefaciens.	
Ano de publicação: 	1994	
Fonte/Imprenta: 	Plant Cell Reports, v.13, p.697-702, 1994.	
Idioma: 	Inglês	
Conteúdo: 	Leaf and stem explants of passiofruit (Passiflora edulis fv flavicarpa) were co-cultivated with a disarmed strain of Agrobacterium tumefaciens harbouring the co-integrate vector pMON200. Four plants of passionfruit were regenerated from leaf explants on agar-sokidified Murashige and Skoog (1962) based medium containing 4,43 uM 6-benzyl-aminopurine and supplemented with 86 uM kanamycin sulphate. The four plants were rooted by transfer to MS based medium with 14,7 uM 3-indolebutyric acid and 2.68 uM a-naphthyleneacetic acid for 7 d, followed by MS based medium lacking growth regulators. Both media used for rooting contained 172 uM kanamycin sulphate. Rooted plants were potted and grown to maturity. Three of the plants synthesised nopaline and expressed neomycin phosphotransferase activity; DNA dot blot and polymerase chain reaction analyses confirmed the presence of the neomycin phosphotransferase gene in three plants.	
Palavras-Chave: 	Agrobacteria; Cultura de tecidos; Passionfruit; Regeneracao de apices; Shoot regeration.	
Thesagro: 	Maracujá; Passiflora Edulis.	
Thesaurus Nal: 	Agrobacterium; tissue culture.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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