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Registro Completo |
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Biblioteca(s): |
Embrapa Acre; Embrapa Amazônia Ocidental. |
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Data corrente: |
02/06/2014 |
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Data da última atualização: |
06/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; SILVA, O. F.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. de S.; BITTENCOURT, D. M. de C.; MILLER, R. N. G. |
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Afiliação: |
Glaucia E. O. Midorikawa, Universidade de Brasília (UnB); MARIA DE LOURDES MENDES DE SOUZA, CTAA; OTNIEL FREITAS SILVA, CTAA; JUREMA DO SOCORRO AZEVEDO DIAS, CPAF-AP; Luis I. B. Kanzaki, Universidade de Brasília (UnB); Rogerio E. Hanada, Instituto Nacional de Pesquisas da Amazônia (Inpa); Renata M. L. C. Mesquita, Instituto Nacional de Pesquisas da Amazônia (Inpa); RIVADALVE COELHO GONCALVES, CPAF-AC; VIRGINIA DE SOUZA ALVARES, CPAF-Acre; DANIELA MATIAS DE C BITTENCOURT, CPAA; Robert N. G. Miller, Universidade de Brasília (UnB). |
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Título: |
Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Microbiology, v.14, n. 138, 2014. |
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ISSN: |
1471-2180 |
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DOI: |
10.1186/1471-2180-14-138 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species. MenosBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius ... Mostrar Tudo |
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Palavras-Chave: |
Castanha do brasil; Castanha-do-brasil; Enfermedades y desórdenes de las plantas; Identificación de patógenos; Nuez del Brasil; Pragas e doenças. |
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Thesagro: |
Aflatoxina; Aspergillus flavus; Bertholletia excelsa; Castanha do pará; Doença de planta; Fungo; Identificação. |
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Thesaurus Nal: |
Amazonia; Aspergillus; Brazil nuts; Fungi; Pathogen identification; Plant diseases and disorders. |
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Categoria do assunto: |
--
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102994/1/castanha-Robert.pdf
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Marc: |
LEADER 03376naa a2200493 a 4500
001 1987473
005 2021-07-06
008 2014 bl uuuu u00u1 u #d
022 $a1471-2180
024 7 $a10.1186/1471-2180-14-138$2DOI
100 1 $aMIDORIKAWA, G. E. O.
245 $aCharacterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level.$h[electronic resource]
260 $c2014
520 $aBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species.
650 $aAmazonia
650 $aAspergillus
650 $aBrazil nuts
650 $aFungi
650 $aPathogen identification
650 $aPlant diseases and disorders
650 $aAflatoxina
650 $aAspergillus flavus
650 $aBertholletia excelsa
650 $aCastanha do pará
650 $aDoença de planta
650 $aFungo
650 $aIdentificação
653 $aCastanha do brasil
653 $aCastanha-do-brasil
653 $aEnfermedades y desórdenes de las plantas
653 $aIdentificación de patógenos
653 $aNuez del Brasil
653 $aPragas e doenças
700 1 $aSOUZA, M. de L. M. de
700 1 $aSILVA, O. F.
700 1 $aDIAS, J. do S. A.
700 1 $aKANZAKI, L. I. B.
700 1 $aHANADA, R. E.
700 1 $aMESQUITA, R. M. L. C.
700 1 $aGONCALVES, R. C.
700 1 $aALVARES, V. de S.
700 1 $aBITTENCOURT, D. M. de C.
700 1 $aMILLER, R. N. G.
773 $tBMC Microbiology$gv.14, n. 138, 2014.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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| Registros recuperados : 16 | |
| 3. |  | KLEEMANN, N.; TORRES, D. P.; RIBEIRO, A. S.; BAMBERG, A. L. Cold finger with semi closed reflux system for sample preparation aiming at Al, Ca, Cr, Cu, Fe, K, Mg, Mn, Ni, V and Zn determination in Drinking Water Treatment Sludge by MIP OES. Analytica Chimica Acta, v. 1096, p. 9-17, Feb. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 7. | | PERIN, E. C.; TORRES, D. P.; BECKER, S.; ROMBALDI, C. V.; SILVEIRA, C. A. P. Planejamento fatorial fracionário para desenvolvimento de método analítico para posterior determinação de arsênio e selênio por gf aas em solos submetidos a remineralizadores de solo. In: CONGRESSO BRASILEIRO DE ROCHAGEM, 3., 2017, Pelotas. Anais... Assis: Triunfal Gráfica e Editora, 2017. 455 p. Editado por Adilson Luis Banberg, Carlos Augusto Posser Silveira, Éder de Souza Martins, Magda Bergmann, Rosane Martinazzo e Suzi Huff Theodoro. p. 45-49| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 9. | | BALDEZ, D. L.; AVILA, L. O.; TORRES, D. P.; MARTINAZZO, R.; SILVEIRA, C. A. P.; VIEIRA, M. A. Determinação de potássio em rochas silicáticas por espectrometria de emissão atômica com chama após dissolução assistida por ultrassom. Quimica Nova, v. 42, n. 10, p. 1095-1100, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 11. | | PANIZ, F. P.; PEDRON, T.; FREIRE, B. M.; TORRES, D. P.; SILVA, F. F.; BATISTA, B. L. Effective procedures for the determination of As, Cd, Cu, Fe, Hg, Mg, Mn, Ni, Pb, Se, Th, Zn, U and rare earth elements in plants and foodstuffs. Analytical Methods, v. 10, p. 4094-4103, 2018.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 12. | | BALDEZ, D. L.; AVILA, L. O.; LISBOA, M. T.; TORRES, D. P.; MARTINAZZO, R.; SILVEIRA, C. A. P.; VIEIRA, M. A. Determinação de K em rochas silicáticas digeridas em ultrassom. In: ENCONTRO DE QUÍMICA DA REGIÃO SUL, 24., 2017, Florianópolis. Anais... Florianópolis: SBQ, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 13. | | NUNES, R. S. G.; VIEIRA, F. C. B.; MARTINAZZO, R.; BAMBERG, A. L.; SILVEIRA, C. A. P.; TORRES, D. P.; FIGUEIREDO, M. C. dos S. The use of retorted oil shale as urea coating does not modify chemical parameters of free-drained soil solution. Research, Society and Development, v. 11, n. 9, e7811931521, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Instrumentação. |
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| 14. | | AVILA, L. O. de; TORRES, D. P.; ORESTE, E. Q.; LISBOA, M. T.; SILVEIRA, C. A. P.; RIBEIRO, A. S.; NUNES, A. M.; MARTINAZZO, R. Speciation of Inorganic Arsenic in Retorted Oil Shale Slurry by Hydride Generation Atomic Absorption Spectrometry (HG AAS) after Ultrasound-Assisted Acid Extraction. Analytical Letters, v. 57, n. 7, p. 1065-1077, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Instrumentação. |
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| 15. | | BAMBERG, A. L.; CAMPOS, A. D.; SILVEIRA, C. A. P.; MARTINS, C. R.; GOMES, C. B.; TORRES, D. P.; ANTHONISEN, D. G.; NAVA, D. E.; NACHTIGAL, G. de F.; HEIDEN, G.; SCHIEDECK, G.; MATTOS, M. L. T.; MARTINAZZO, R. I Plano Diretor do Núcleo de Insumos 2018-2028. Pelotas: Embrapa Clima Temperado, 2018. 23 p. (Embrapa Clima Temperado. Documentos, 454.)| Biblioteca(s): Embrapa Clima Temperado. |
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| 16. | | SILVA, J. T. da; PANIZ, F. P.; SANCHEZ, F. e. S.; PEDRON, T.; TORRES, D. P.; CONCENÇO, F. I. G. da R.; PARFITT, J. M. B.; BATISTA, B. L. Selected soil water tensions at phenological phases and mineral content of trace elements in rice grains - mitigating arsenic by water management. Agricultural Water Management, v. 228, n. 20, Feb. 2020, 105884.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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