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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
16/11/2018 |
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Data da última atualização: |
11/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALESSI, A. M.; BIRD, S. M.; OATES, N. C.; LI, Y.; DOWLE, A. A.; NOVOTNY, E. H.; AZEVEDO, E. R. de; BENNETT, J. P.; POLIKARPOV, I.; YOUNG, J. P. W.; MCQUEEN-MASON, S. J.; BRUCE, N. C. |
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Afiliação: |
ANNA M. ALESSI, UNIVERSITY OF YORK; SUSANNAH M. BIRD, UNIVERSITY OF YORK; NICOLA C. OATES, UNIVERSITY OF YORK; YI LI, UNIVERSITY OF YORK; ADAM A. DOWLE, UNIVERSITY OF YORK; ETELVINO HENRIQUE NOVOTNY, CNPS; EDUARDO R. DE AZEVEDO, USP; JOSEPH P. BENNETT, UNIVERSITY OF YORK; IGOR POLIKARPOV, USP; J. PETER W. YOUNG, UNIVERSITY OF YORK; SIMON J. MCQUEEN-MASON, UNIVERSITY OF YORK; NEIL C. BRUCE, UNIVERSITY OF YORK. |
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Título: |
Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biotechnology for Biofuels, v. 11, article 166, 2018. |
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DOI: |
https://doi.org/10.1186/s13068-018-1164-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. MenosBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacau... Mostrar Tudo |
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Palavras-Chave: |
CAZy; Metasecretome. |
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Thesaurus Nal: |
Lignocellulose. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186133/1/2018-044.pdf
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Marc: |
LEADER 03644naa a2200301 a 4500
001 2099501
005 2021-11-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s13068-018-1164-2$2DOI
100 1 $aALESSI, A. M.
245 $aDefining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies.$h[electronic resource]
260 $c2018
520 $aBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation.
650 $aLignocellulose
653 $aCAZy
653 $aMetasecretome
700 1 $aBIRD, S. M.
700 1 $aOATES, N. C.
700 1 $aLI, Y.
700 1 $aDOWLE, A. A.
700 1 $aNOVOTNY, E. H.
700 1 $aAZEVEDO, E. R. de
700 1 $aBENNETT, J. P.
700 1 $aPOLIKARPOV, I.
700 1 $aYOUNG, J. P. W.
700 1 $aMCQUEEN-MASON, S. J.
700 1 $aBRUCE, N. C.
773 $tBiotechnology for Biofuels$gv. 11, article 166, 2018.
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Registro original: |
Embrapa Solos (CNPS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
23/09/2002 |
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Data da última atualização: |
13/03/2017 |
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Autoria: |
SANTOS, S. A.; SOUZA, G. S. e; CRISPIM, S. M. A.; COSTA, C.; COMASTRI FILHO, J. A. |
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Afiliação: |
EMBRAPA Pantanal (Corumba, MS). EMBRAPA Sede / SAE (Brasilia, DF). UNESP/FMVZ (Botucatu, SP). |
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Título: |
Curva de crescimento de bezerros Nelore criados em pastagem nativa na sub-regiao da Nhecolandia, Pantanal. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 39., 2002, Recife. A producao animal e a sociedade brasileira. Anais. Recife: UFRPE / SBZ, 2002. CD-ROM. (Secao Forragicultura). Nao paginado. |
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Idioma: |
Português |
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Conteúdo: |
O proposito deste estudo foi ajustar dados de peso e altura da anca de 20 bezerros Nelo-
re, do nascimento ate 10-11 meses de idade a uma regressao logistica. As medidas foram
realizadas mensalmente durante o periodo de outubro de 1997 a setembro de 1999, com-
preendendo dois anos hidrologicos (1997/98 e 1998/99). Os bezerros foram mantidos
com a mae ate 10-11 meses de idade, em pastagens nativ as, na sub-regiao da Nheco-
landia. Avaliou-se o efeito de ano hidrologico de nascimento e sexo sobre os parametros
da funcao logistica e estimou-se o ponto de inflexao da curva de crescimento em peso.
As estimativas de peso e altura da anca aos 10-11 meses de idade (parametro a) foram
de 168,8 kg e 124,1 cm, respectivamente. Estimativas do indice de maturidade (parame-
tro c) foram de -0,016 e -0,011 para peso e altura da anca, respectivamente. O ponto de
inflexao do peso medio dos bezerros foi aos 93,5 dias de idade. Somente ano hidrologico
de nascimento teve efeito significativo sobre os parametros do modelo. Os bezerros nas-
cidos em 1997/98 apresentaram peso mais baixo aos 205 dias de idade e peso mais alto
aos 10-11 meses de idade do que bezerros nascidos em 1998/99, indicando que a quantidade/qualidade das pastagens nativas da sub-regiao da Nhecolandia para o de-
sempenho ponderal dos bezerros teve maior importancia apos o mes de maio. |
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Palavras-Chave: |
Altura da anca; Height at hip; Logist regression; Producao de vitelo; Regressao logistica; Veal production. |
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Thesagro: |
Bezerro; Peso. |
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Thesaurus NAL: |
calves; Pantanal; weight. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02326naa a2200301 a 4500
001 1810346
005 2017-03-13
008 2002 bl --- 0-- u #d
100 1 $aSANTOS, S. A.
245 $aCurva de crescimento de bezerros Nelore criados em pastagem nativa na sub-regiao da Nhecolandia, Pantanal.
260 $c2002
520 $aO proposito deste estudo foi ajustar dados de peso e altura da anca de 20 bezerros Nelo- re, do nascimento ate 10-11 meses de idade a uma regressao logistica. As medidas foram realizadas mensalmente durante o periodo de outubro de 1997 a setembro de 1999, com- preendendo dois anos hidrologicos (1997/98 e 1998/99). Os bezerros foram mantidos com a mae ate 10-11 meses de idade, em pastagens nativ as, na sub-regiao da Nheco- landia. Avaliou-se o efeito de ano hidrologico de nascimento e sexo sobre os parametros da funcao logistica e estimou-se o ponto de inflexao da curva de crescimento em peso. As estimativas de peso e altura da anca aos 10-11 meses de idade (parametro a) foram de 168,8 kg e 124,1 cm, respectivamente. Estimativas do indice de maturidade (parame- tro c) foram de -0,016 e -0,011 para peso e altura da anca, respectivamente. O ponto de inflexao do peso medio dos bezerros foi aos 93,5 dias de idade. Somente ano hidrologico de nascimento teve efeito significativo sobre os parametros do modelo. Os bezerros nas- cidos em 1997/98 apresentaram peso mais baixo aos 205 dias de idade e peso mais alto aos 10-11 meses de idade do que bezerros nascidos em 1998/99, indicando que a quantidade/qualidade das pastagens nativas da sub-regiao da Nhecolandia para o de- sempenho ponderal dos bezerros teve maior importancia apos o mes de maio.
650 $acalves
650 $aPantanal
650 $aweight
650 $aBezerro
650 $aPeso
653 $aAltura da anca
653 $aHeight at hip
653 $aLogist regression
653 $aProducao de vitelo
653 $aRegressao logistica
653 $aVeal production
700 1 $aSOUZA, G. S. e
700 1 $aCRISPIM, S. M. A.
700 1 $aCOSTA, C.
700 1 $aCOMASTRI FILHO, J. A.
773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 39., 2002, Recife. A producao animal e a sociedade brasileira. Anais. Recife: UFRPE / SBZ, 2002. CD-ROM. (Secao Forragicultura). Nao paginado.
Download
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