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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
FUGANTI, R.; BENEVENTI, M. A.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; NEPOMUCENO, A. L. |
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Título: |
Microsatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 321. |
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Série: |
(Embrapa Soja. Documentos, 228).
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
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Conteúdo: |
It is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this marker amplification was cloned, sequenced, and checked for homology by using the NCBI Blast. The analyzed sequence showed 100% homology with the promoter region of a heat-shock protein (HSP) found in soybean, Gmhsp17.6-L (M11317). Primers were designed using the entire gene sequence available in the GenBank, with the objective of generating bands in both resistant and susceptible genotypes. All resulting fragments were sequenced and showed homology with the promoter region of the Gmhsp17.6-L gene. We observed a microsatellite region (ATn repetitions) inside the promoter region of this gene, but the number of repetitions was different in each genotype tested, suggesting a possible correlation between the insertion length in the promoter and resistance or susceptibility to the nematode. After that, aimingto check the expression of this gene, a RPA (Ribonuclease Protection Assay) was performed. The results showed that both resistant and susceptible genotypes seemed to express the Gmhsp 17.6-L gene. However, the length of the microsatellite insertion in the promoter region might alter the levels of expression. MenosIt is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this m... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 03691naa a2200229 a 4500
001 1466852
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aFUGANTI, R.
245 $aMicrosatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica.
260 $c2004
300 $ap. 321.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aIt is estimated that 11% of annual global losses in production of soybean [Glycine max (L.) Merr.] is caused by nematode parasitism. In Brazil, nematode species in the genus Meloidogyne represent a serious yield constraint in many regions. Control measures, such as the development of resistant cultivars, are essential for the maintenance of acceptable productivity levels. Therefore, with the objective of identifying genetic polymorphisms between resistant and susceptible genotypes to the root knot nematode, Meloidogyne javanica, a population of twenty-five highly resistant lines and twenty-six highly susceptible lines, derived from a cross between resistant (PI 595099) and susceptible (BRS 133) genotypes were tested for microsatellite markers (SSR). Significant polymorphic markers were cloned and sequenced in an attempt to identify possible genomic regions responsible for the reaction to the nematode. Among 97 loci initially analyzed, 21 were polymorphic between the parents and seven showed high differential frequency (P<0,10) into the resistant population. In the F linkage group, where other quantitative trait loci (QTLs) conferring resistance to nematodes have already been reported; the SSR loci 176 Soy HSP, Satt 114 and Satt 423 showed significant correlation with the number of galls observed on soybean roots. The QTL analysis in this group showed the presence of at least one gene located next to the 176 Soy HSP marker, with a Lod of 27.5. The resulting fragment of this marker amplification was cloned, sequenced, and checked for homology by using the NCBI Blast. The analyzed sequence showed 100% homology with the promoter region of a heat-shock protein (HSP) found in soybean, Gmhsp17.6-L (M11317). Primers were designed using the entire gene sequence available in the GenBank, with the objective of generating bands in both resistant and susceptible genotypes. All resulting fragments were sequenced and showed homology with the promoter region of the Gmhsp17.6-L gene. We observed a microsatellite region (ATn repetitions) inside the promoter region of this gene, but the number of repetitions was different in each genotype tested, suggesting a possible correlation between the insertion length in the promoter and resistance or susceptibility to the nematode. After that, aimingto check the expression of this gene, a RPA (Ribonuclease Protection Assay) was performed. The results showed that both resistant and susceptible genotypes seemed to express the Gmhsp 17.6-L gene. However, the length of the microsatellite insertion in the promoter region might alter the levels of expression.
700 1 $aBENEVENTI, M. A.
700 1 $aSILVA, J. F. V.
700 1 $aARIAS, C. A. A.
700 1 $aMARIN, S. R. R.
700 1 $aBINNECK, E.
700 1 $aNEPOMUCENO, A. L.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 169 | |
| 61. |  | PASSIANOTTO, A. L. de L.; KUWAHARA, M. K.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; BINNECK, E.; GONELA, A.; MARCELINO, F. C. Desenvolvimento e validação do sistema de genotipagem molecular de cultivares de soja via sequenciador automático com marcadores microssatélites. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 209-213. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 62. | | PASSIANOTTO, A. L. de L.; LOPES, V. S.; RINCÃO, M. P.; NEPOMUCENO, A. L.; BINNECK, E.; ABDELNOOR, R. V.; GONELA, A.; MARCELINO, F. C. Desenvolvimento e validação do sistema de genotipagem molecular com marcadores microssatélites de cultivares de soja via sequenciador automático. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia. Charles Darwin: a origem das espécies: o livro que transformou a humanidade. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p.138.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 63. | | PASSIANOTTO, A. L. de L.; SILLA, P. R.; MARIN, S. R. R.; KUWAHARA, M. K.; NEPOMUCENO, A. L.; BINNECK, E.; ABDELNOOR, R. V.; GONELA, A.; MARCELINO, F. C. Determinação de perfis genéticos de cultivares de soja desenvolvidos pela Embrapa. In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... [Curitiba]: UFPR, 2010. p. 166.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 64. | | NEIVA, M. M.; GODOY, S. M.; FELICIANO, D. C.; SILVA, J. F. M.; BINNECK, E.; ROSA, R. da; MURÚA, M. G.; SPECHT, A.; SOSA_GOMEZ. Development of microsatellite markers and genetic diversity of the velvetbean caterpillar Anticarsia gemmatalis(Lepidoptera: Erebidae). Austral Entomology, v. 62, p. 345-459, 2023. p. 345-349| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 65. | | NEIVA, M. M.; GODOY, S. M.; FELICIANO, D. C.; SILVA, J. F. M.; BINNECK, E.; ROSA, R. da; MURÚA, M. G.; SPECHT, A.; SÓSA-GOMEZ, D. R. Development of microsatellite markers and genetic diversity of the velvetbean caterpillar Anticarsia gemmatalis(Lepidoptera: Erebidae). Austral Entomology, v. 62, p. 345-459, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Soja. |
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| 66. | | FUGANTI, R.; BENEVENTI, M. A.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; NEPOMUCENO, A. L. Microsatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 321. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 68. | | BENEVENTI, M. A.; FUGANTI, R.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; LIMA, C. G.; NEPOMUCENO, A. L. Microsatellite molecular markers associated with selection process of soybean resistant genotypes to Meloidogyne javanica and Heterodera glycines. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 321-322. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 69. | | BRETON, M. C.; RUAS, C. F.; NEUMAIER, N.; MARTINS, P. K.; PEDROSO, J. C.; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEPOMUCENO, A. L. Respostas moleculares de cultivares de soja [Glycine max (L.) Merrill] ao fotoperíodo. In: CONGRESSO NACIONAL DE GENÉTICA, 48., 2002, Águas de Lindóia. A genética na inclusão social: resumos. Águas de Lindóia: SBG, 2002. 1 CD-ROM. Resumo Área MG pdf.090.| Biblioteca(s): Embrapa Soja. |
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| 70. | | SOUZA, M. C. P. de; SILVA, M. D. da; BINNECK, E.; CABRAL, G. A. de L.; ISEPPON, A. M. B.; POMPELLI, M. F.; ENDRES, L.; KIDO, E. A. RNA-Seq transcriptome analysis of Jatropha curcas L. accessions after salt stimulus and unigene-derived microsatellite mining. Industrial Crops & Products, v. 147, 112168, 2020. 13 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 71. | | PEDROSO, J. C.; SILVA, J. F. V.; MARTINS, P. K.; BRETON, M. C.; BINNECK, E.; MARIN, S. R. R.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. Utilização da técnica de "differential display" na busca de genes de resistência a nematóide da galha (Meloidogyne javanica) em genótipos de soja (Glycine max L. Merrill). In: CONGRESSO NACIONAL DE GENÉTICA, 48., 2002, Águas de Lindóia. A genética na inclusão social: resumos. Águas de Lindóia: SBG, 2002. 1 CD-ROM. Resumo Área MG pdf.092.| Biblioteca(s): Embrapa Soja. |
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| 72. | | BINNECK, E.; NASCIMENTO, L. S.; ABDELNOOR, R. V.; ALMEIDA, A. M. R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; HUNGRIA, M. Using soybean gene express for gene expression profiling in soybean. In: WORLD SOYBEAN RESEARCH CONFERENCE, 9., 2013, Durban. [Proceedings...]. Durban: OPDT: OPOT, 2013. Abst. 249. 1 CD-ROM. WSRC.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 73. | | LEMOS, N. G.; MOLINA, J. C.; STOLF, R.; MORALES, A. M. R.; MARIN, S. R. R.; BINNECK, E, FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. Expressão de aquaporinas e outros genes em raízes de soja sob condições de deficiência hídrica. In: CONGRESSO NACIONAL DE GENÉTICA , 49., 2003, Águas de Lindóia. A dupla hélice do DNA: [resumos]. [S. l.]: SBG, 2003. 1 CD-ROM. Resumo Área GP pdf. 069.| Biblioteca(s): Embrapa Soja. |
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| 74. | | BRETON, M. N.; RUAS, C. F.; NEUMAIER, N.; MARTINS, P. K.; PEDROSO, J. C.; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEPOMUCENO, A. L. Expressão diferencial de genes em plantas de soja [Glycine max (L.) Merrill] submetidas a dois fotoperíodos. In: CONGRESSO BRASILEIRO DE SOJA, 2.; MERCOSOJA 2002, 2002, Foz do Iguaçu. Perspectivas do agronegócio da soja: resumos. Londrina: Embrapa Soja, 2002. p. 19. (Embrapa Soja. Documentos, 181). Organizado por Odilon Ferreira Saraiva, Clara Beatriz Hoffmann-Campo.| Biblioteca(s): Embrapa Soja. |
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| 75. | | MOLINA, J. C.; LEMOS, N. G.; MORALES, A. M.; MARIN, S. R. R.; BINNECK, E.; FARIAS, J. R. B.; NEUMAIER, N.; NEPOMUCENO, A. L. Expressão gênica em raízes de soja durante o déficit hídrico. In: CONGRESSO NACIONAL DE GENÉTICA , 49., 2003, Águas de Lindóia. A dupla hélice do DNA: [resumos]. [S. l.]: SBG, 2003. 1 CD-ROM. Resumo Área GP pdf. 068.| Biblioteca(s): Embrapa Soja. |
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| 76. | | PEREIRA, S. S.; STOLF, R.; ROLLA, A. A. P.; FUGANTI, R.; MARIN, S. R. R.; BINNECK, E.; ABDELNOOR, R. V.; MARCELINO, F. C.; NEPOMUCENO, A. L. Expression analysis of transcription factors involved in drought tolerance in soybean roots. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 2., 2009, Búzios. Programa e resumos. [S.l.]: SBG, 2009. p. 160.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 77. | | COSTAMILAN, L. M.; YORINORI, J. T.; ALMEIDA, A. M. R.; SEIXAS, C. D. S.; BINNECK, E.; ARAÚJO, M. R.; CARBONARI, J. A. First report of Diaporthe phaseolorum var. caulivora infecting soybean plants in Brazil. Tropical Plant Pathology, Brasília, DF, v. 33, n. 5, p. 381-385, Sep./Oct. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 78. | | KIDO, E. A.; FERREIRA-NETO, J. R. C.; BINNECK, E.; SILVA, M. da; SILVA JUNIOR, W. da; BENKO-ISEPPON, A. M. Explore the RNA-sequencing and the next-generation sequencing in crops responding to abiotic stress. In: SHARMA, P.; YADAV, D.; GAUR, R. K. (ed.). Bioinformatics in Agriculture: Next Generation Sequencing Era. [S. l.]: Elsevier Academic Press, c2022. CHAP. 10, p. 161-175.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Soja. |
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| 79. | | ALMEIDA, A. M. R.; SOSA-GOMEZ, D. R.; BINNECK, E.; MARIN, S. R. R.; ZUCCHI, M. I. ABDELNOOR, R. V.; SOUTO, E. R. Effect of crop rotation on specialization and genetic diversity of Macrophomina phaseolina. Tropical Plant Pathology, Brasília, DF, v. 33, n. 4, p. 257-264, jul./aug. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 169 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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