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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
24/02/2017 |
Data da última atualização: |
30/08/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GIGLIOTI, R.; OLIVEIRA, H. N.; IBELLI, A. M. G.; BILHASSI, T. B.; NEO, T. A.; SANTANA, C. H.; RABELO, M. D.; MACHADO, R. Z.; CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S. |
Afiliação: |
Rodrigo Giglioti, UNESP; Henrique Nunes Oliveira, UNESP; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; Talita Barban Bilhassi, UNESP; Thalita Athiê Néo, UFSCar; Clarissa Helena Santana, UNESP; MARCIO DIAS RABELO, CPPSE; Rosângela Zacarias Machado, UNESP; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
Título: |
Neither quantification by qPCR nor quantitative Elisa can be used to discriminate angus cattle for resistance/susceptibility to Babesia bovis. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 8, n. 3, p. 335-340, 2017. |
DOI: |
http://dx.doi.org/10.1016/j.ttbdis.2016.11.008 |
Idioma: |
Inglês |
Conteúdo: |
With the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtainedshowedthehighsensitivity ofthe techniques, with100%ofthe animals beingpositive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis. MenosWith the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtainedshowedthehighsensitivity ofthe techniques, with100%ofthe animals beingpositive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC ... Mostrar Tudo |
Palavras-Chave: |
Resistance. |
Thesagro: |
Babesia Bovis. |
Thesaurus Nal: |
beef cattle. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02829naa a2200277 a 4500 001 2065568 005 2018-08-30 008 2017 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1016/j.ttbdis.2016.11.008$2DOI 100 1 $aGIGLIOTI, R. 245 $aNeither quantification by qPCR nor quantitative Elisa can be used to discriminate angus cattle for resistance/susceptibility to Babesia bovis.$h[electronic resource] 260 $c2017 520 $aWith the aim of finding quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to Babesia bovis, we estimated the repeatability and correlation between the level of infection, determined by the number of copies of a fragment of the gene that encodes cytochrome B (NC mt-cyB) of B. bovis, and the levels of the anti-B. bovis antibodies, in blood samples collected from 51 Angus cattle on two different occasions. Samples with the anticoagulant EDTA were used for DNA extraction and without anticoagulant for separation of the blood serum. The quantification of the NC mt-cyB of B. bovis was carried out by the quantitative PCR technique (qPCR), while the anti-B. bovis IgG antibody titers (S/P) were quantified by the ELISA method. The NC and S/P data were log10-transformed to improve the approximation to the normal distribution and were analyzed using mixed models. The correlations between NC mt-cyB and S/P were estimated, as well as the repeatability values for each trait. The results obtainedshowedthehighsensitivity ofthe techniques, with100%ofthe animals beingpositive for B. bovis, detected by both the serological and molecular tests. The correlations estimated between NC and S/P were low, 0.10 and 0.12, in the first and second collection, respectively. The repeatability estimated for NC was 0.06, whereas for the S/P it was 0.42. The low correlations between S/P and NC in the two collections demonstrated that the variation in the NC value is independent of the level of antibodies. This results indicated that animals with a higher levels of antibodies against B. bovis in the first collection continued to have a higher levels in the second one. However, the very low values for the repeatability value of NC, and for the correlations between S/P and NC, demonstrates that neither NC or S/P could be used to discriminate animals for resistance/susceptibility to B. bovis. 650 $abeef cattle 650 $aBabesia Bovis 653 $aResistance 700 1 $aOLIVEIRA, H. N. 700 1 $aIBELLI, A. M. G. 700 1 $aBILHASSI, T. B. 700 1 $aNEO, T. A. 700 1 $aSANTANA, C. H. 700 1 $aRABELO, M. D. 700 1 $aMACHADO, R. Z. 700 1 $aCHAGAS, A. C. de S. 700 1 $aOLIVEIRA, M. C. de S. 773 $tTicks and Tick-borne Diseases$gv. 8, n. 3, p. 335-340, 2017.
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Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
28/10/2008 |
Data da última atualização: |
02/10/2012 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
LEITE, M. S. P.; IEDE, E. T.; PENTEADO, S. do R. C.; ZALESKY, S. R. M.; CAMARGO, J. M. M.; RIBEIRO, R. D. |
Afiliação: |
Maria Silvia Pereira Leite, TURFAL; Edson Tadeu Iede, Embrapa Florestas; Susete do Rocio Chiarello Penteado, Embrapa Florestas; Scheila Ribeiro Messa Zaleski, UFPR; Joelma Melissa Malherbe Camargo, UFPR; Rodrigo Daniel Ribeiro, UFPR. |
Título: |
Persistência de Beauveria bassiana (Bals) Vuill, em plantas de erva-mate para o controle de Hedypathes betulinus (Klug, 1825) (Coleoptera: cerambycidae). |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008. Uberlândia, 2008. Ciência, Tecnologia e Inovação. Sociedade Brasileira de Entomologia: Uberlândia, 2008. |
Idioma: |
Português |
Notas: |
Pôster. |
Conteúdo: |
A broca-da-erva-mate, Hedypathes betulinus, é a principal prga da cultura de erva-mate. O fungo Beauveria bassiana apresenta um grande potencial de supressão populacional da praga. O objetivo deste trabalho foi avaliar a persistência deste isolado na cultura da erva-mate, visando o controle deste cerambicídeo. |
Palavras-Chave: |
Broca-da-erva-mate; Erva-mate; Fungo entomotogênico. |
Thesagro: |
Beauveria Bassiana; Controle Biológico; Hedypathes Betulinus; Ilex Paraguariensis; Praga. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/67244/1/SP5404.pdf
|
Marc: |
LEADER 01312nam a2200277 a 4500 001 1314941 005 2012-10-02 008 2008 bl uuuu u00u1 u #d 100 1 $aLEITE, M. S. P. 245 $aPersistência de Beauveria bassiana (Bals) Vuill, em plantas de erva-mate para o controle de Hedypathes betulinus (Klug, 1825) (Coleoptera$bcerambycidae).$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008. Uberlândia, 2008. Ciência, Tecnologia e Inovação. Sociedade Brasileira de Entomologia: Uberlândia$c2008 500 $aPôster. 520 $aA broca-da-erva-mate, Hedypathes betulinus, é a principal prga da cultura de erva-mate. O fungo Beauveria bassiana apresenta um grande potencial de supressão populacional da praga. O objetivo deste trabalho foi avaliar a persistência deste isolado na cultura da erva-mate, visando o controle deste cerambicídeo. 650 $aBeauveria Bassiana 650 $aControle Biológico 650 $aHedypathes Betulinus 650 $aIlex Paraguariensis 650 $aPraga 653 $aBroca-da-erva-mate 653 $aErva-mate 653 $aFungo entomotogênico 700 1 $aIEDE, E. T. 700 1 $aPENTEADO, S. do R. C. 700 1 $aZALESKY, S. R. M. 700 1 $aCAMARGO, J. M. M. 700 1 $aRIBEIRO, R. D.
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