Data/hora: 03/06/2024 05:07:00

Biblioteca(s): | Embrapa Instrumentação.
Data corrente: | 23/03/2022
Data da última atualização: | 24/01/2024
Autoria: | SQUINCA, P.; BILATTO, S.; BADINO, A. C.; FARINAS, C. S.
Afiliação: | CRISTIANE SANCHEZ FARINAS, CNPDIA.
Título: | Nanocellulose production in future biorefineries: An integrated approach using Tailor-Made enzymes.
Ano de publicação: | 2022
Fonte/Imprenta: | ACS Sustainable Chemistry & Engineering, v. 8, 2020.
Páginas: | 2277–2286
DOI: | https://dx.doi.org/10.1021/acssuschemeng.9b06790
Idioma: | Inglês
Conteúdo: | The development of process engineering approaches to integrate the production of biofuels and high value-added biobased products, such as enzymes and nanocellulose, is crucial to improve the financial performance and sustainability of lignocellulosic biomass biorefineries. Here, the feasibility of applying enzymes produced on-site to obtain nanocellulose was evaluated using eucalyptus cellulose pulp as a model feedstock. A systematic analysis of the structural properties of the nanomaterials obtained after hydrolysis using a cellulolytic enzymatic complex with high endoglucanase specific activity (17.09 IU/mgprotein), produced by Aspergillus niger, followed by sonication, revealed that longer ball milling pretreatment and reaction times favored extraction of the cellulose nanocrystals (CNCs). The highest yield (24.6%) of CNCs was achieved using 96 h of enzymatic hydrolysis of the ball-milled cellulose pulp, followed by sonication for 5 min. The CNCs presented approximate lengths of 294.0 nm and diameters of 24.0 nm, and the crystallinity index increased from 57.5% to 78.3%, compared to the cellulose pulp that was only ball milled. These findings demonstrated that nanocelluloses could be successfully extracted using on-site produced enzymes and that the sustainable integrated process reported here could contribute to the development of the nascent biobase economy.
Palavras-Chave: | Cellulose nanocrystals; Integrated biorefinery; On-site production; Solid-state fermentation.
Categoria do assunto: | --
Registro original: | Embrapa Instrumentação (CNPDIA)
Exemplares: | CNPDIA - (17949-1) - UPC - AP - DD - PROCI.22/17 -  - 2022/17 - DIS