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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/06/2012 |
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Data da última atualização: |
24/07/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BELARMINO, L. C.; OLIVEIRA, A. R. da S.; BRASILEIRO-VIDAL, A. C.; BORTOLETI, K. C. de A.; BEZERRA-NETO, J. P.; ABDELNOOR, R. V.; BENKO-ISEPPON, A. M. |
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Afiliação: |
LUIS C. BELARMINO, UFPE; ANA R. DA S. OLIVEIRA, UFPE; ANA C. BRASILEIRO-VIDAL, UFPE; KYRIA C. DE A. BORTOLETI, UFPE / Univ. Federal do Vale do São Francisco; JOÃO PACÍFICO BEZERRA-NETO, UFPE; RICARDO VILELA ABDELNOOR, CNPSO; ANA M. BENKO-ISEPPON, UFPE. |
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Título: |
Mining plant genome browsers as a means for efficient connection of physical, genetic and cytogenetic mapping: an example using soybean. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 335-347, May 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Physical maps are important tools to uncover general chromosome structure as well as to compare different plant lineages and species, helping to elucidate genome structure, evolution and possibilities regarding synteny and colinearity. The increasing production of sequence data has opened an opportunity to link information from mapping studies to the underlying sequences. Genome browsers are invaluable platforms that provide access to these sequences, including tools for genome analysis, allowing the integration of multivariate information, and thus aiding to explain the emergence of complex genomes. The present work presents a tutorial regarding the use of genome browsers to develop targeted physical mapping, providing also a general overview and examples about the possibilities regarding the use of Fluorescent In Situ Hybridization (FISH) using bacterial artificial chromosomes (BAC), simple sequence repeats (SSR) and rDNA probes, highlighting the potential of such studies for map integration and comparative genetics. As a case study, the available genome of soybean was accessed to show how the physical and in silico distribution of such sequences may be compared at different levels. Such evaluations may also be complemented by the identification of sequences beyond the detection level of cytological methods, here using members of the aquaporin gene family as an example. The proposed approach highlights the complementation power of the combination of molecular cytogenetics and computational approaches for the anchoring of coding or repetitive sequences in plant genomes using available genome browsers, helping in the determination of sequence location, arrangement and number of repeats, and also filling gaps found in computational pseudochromosome assemblies. MenosPhysical maps are important tools to uncover general chromosome structure as well as to compare different plant lineages and species, helping to elucidate genome structure, evolution and possibilities regarding synteny and colinearity. The increasing production of sequence data has opened an opportunity to link information from mapping studies to the underlying sequences. Genome browsers are invaluable platforms that provide access to these sequences, including tools for genome analysis, allowing the integration of multivariate information, and thus aiding to explain the emergence of complex genomes. The present work presents a tutorial regarding the use of genome browsers to develop targeted physical mapping, providing also a general overview and examples about the possibilities regarding the use of Fluorescent In Situ Hybridization (FISH) using bacterial artificial chromosomes (BAC), simple sequence repeats (SSR) and rDNA probes, highlighting the potential of such studies for map integration and comparative genetics. As a case study, the available genome of soybean was accessed to show how the physical and in silico distribution of such sequences may be compared at different levels. Such evaluations may also be complemented by the identification of sequences beyond the detection level of cytological methods, here using members of the aquaporin gene family as an example. The proposed approach highlights the complementation power of the combination of molecular cytogenetics ... Mostrar Tudo |
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Palavras-Chave: |
Bioinformática; FISH, BAC, SSR. |
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Thesagro: |
Gene; Genoma; Soja. |
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Thesaurus Nal: |
Aquaporins; Bioinformatics; Genes; Genome; Soybeans. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/61121/1/gmb.mining.v35n1s.335-347.2012.pdf
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Marc: |
LEADER 02722naa a2200313 a 4500
001 1926648
005 2012-07-24
008 2012 bl uuuu u00u1 u #d
100 1 $aBELARMINO, L. C.
245 $aMining plant genome browsers as a means for efficient connection of physical, genetic and cytogenetic mapping$ban example using soybean.
260 $c2012
520 $aPhysical maps are important tools to uncover general chromosome structure as well as to compare different plant lineages and species, helping to elucidate genome structure, evolution and possibilities regarding synteny and colinearity. The increasing production of sequence data has opened an opportunity to link information from mapping studies to the underlying sequences. Genome browsers are invaluable platforms that provide access to these sequences, including tools for genome analysis, allowing the integration of multivariate information, and thus aiding to explain the emergence of complex genomes. The present work presents a tutorial regarding the use of genome browsers to develop targeted physical mapping, providing also a general overview and examples about the possibilities regarding the use of Fluorescent In Situ Hybridization (FISH) using bacterial artificial chromosomes (BAC), simple sequence repeats (SSR) and rDNA probes, highlighting the potential of such studies for map integration and comparative genetics. As a case study, the available genome of soybean was accessed to show how the physical and in silico distribution of such sequences may be compared at different levels. Such evaluations may also be complemented by the identification of sequences beyond the detection level of cytological methods, here using members of the aquaporin gene family as an example. The proposed approach highlights the complementation power of the combination of molecular cytogenetics and computational approaches for the anchoring of coding or repetitive sequences in plant genomes using available genome browsers, helping in the determination of sequence location, arrangement and number of repeats, and also filling gaps found in computational pseudochromosome assemblies.
650 $aAquaporins
650 $aBioinformatics
650 $aGenes
650 $aGenome
650 $aSoybeans
650 $aGene
650 $aGenoma
650 $aSoja
653 $aBioinformática
653 $aFISH, BAC, SSR
700 1 $aOLIVEIRA, A. R. da S.
700 1 $aBRASILEIRO-VIDAL, A. C.
700 1 $aBORTOLETI, K. C. de A.
700 1 $aBEZERRA-NETO, J. P.
700 1 $aABDELNOOR, R. V.
700 1 $aBENKO-ISEPPON, A. M.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 35, n. 1, suppl., p. 335-347, May 2012.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
02/12/2022 |
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Data da última atualização: |
09/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SENA, I. S.; FERREIRA, A. M.; MARINHO, V. H.; HOLANDA, F. H. E.; BORGES, S. F.; SOUZA, A. A. de; KOGA, R. de C. R.; LIMA, A. L.; FLORENTINO, A. C.; FERREIRA, I. M. |
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Afiliação: |
IRACIREMA S. SENA, UNIVERSIDADE FEDERAL DO AMAPÁ; ADRIANA M. FERREIRA, UNIVERSIDADE FEDERAL DO AMAPÁ; VICTOR H. MARINHO, UNIVERSIDADE FEDERAL DO AMAPÁ; FABRÍCIO H. E HOLANDA, UNIVERDADE FEDERAL DO AMAPÁ; SWANNY F. BORGES, UNIVERSIDADE FEDERAL DO AMAPÁ; AGERDANIO A. DE SOUZA, UNIVERSIDADE FEDERAL DO AMAPÁ; ROSEMARY DE CARVALHO R. KOGA, UNIVERSIDADE FEDERAL DO AMAPÁ; ADILSON LOPES LIMA, CPAF-AP; ALEXANDRO C. FLORENTINO, UNIVERSIDADE FEDERAL DO AMAPÁ; IRLON M. FERREIRA, UNIVERSIDADE FEDERAL DO AMAPÁ. |
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Título: |
Euterpe oleracea Mart (açaizeiro) from the Brazilian Amazon: a novel font of fungi for Lipase production. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Microorganisms, v. 10, p. 2394, 2022. |
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DOI: |
https://doi.org/10.3390/microorganisms10122394 |
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Idioma: |
Inglês |
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Conteúdo: |
Lipases (EC 3.1.1.3) are hydrolases that catalyze triglycerides hydrolysis in free fatty acids and glycerol. Among the microorganisms that produce lipolytic enzymes, the entophytic fungi stand out. We evaluated 32 fungi of different genera, Pestalotiopsis, Aspergillus, Trichoderma, Penicillium, Fusarium, Colletotrichum, Chaetomium, Mucor, Botryodiplodia, Xylaria, Curvularia, Neocosmospora and Verticillium, isolated from Euterpe oleracea Mart. (Açaizeiro) from the Brazilian Amazon for lipase activity. The presence of lipase was evidenced by the deposition of calcium crystals. The endophytic Pestalotiopsis sp. (31) and Aspergillus sp. (24) with Pz 0.237 (++++) and 0.5 (++++), respectively, were the ones that showed the highest lipolytic activity in a solid medium. Lipase activity was rated in liquid medium, in a different range of temperatures (?C), pH and time (days). The values obtained in the production of lipase by the endophytic fungi were 94% for Pestalotiopsis sp. (31) and 93.87% for Aspergillus sp. (24). Therefore, it is emphasized that the endophytic fungus isolated the E. oleracea palm may be a potential candidate to produce enzymes of global commercial interest. |
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Thesagro: |
Enzima; Vegetal Inferior. |
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Thesaurus NAL: |
Microorganisms. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1149132/1/CPAF-AP-2022-Euterpe-oleracea-Mart-Acaizeiro-from-the-Brazilian-Amazon.pdf
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Marc: |
LEADER 02020naa a2200277 a 4500
001 2149132
005 2022-12-09
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/microorganisms10122394$2DOI
100 1 $aSENA, I. S.
245 $aEuterpe oleracea Mart (açaizeiro) from the Brazilian Amazon$ba novel font of fungi for Lipase production.$h[electronic resource]
260 $c2022
520 $aLipases (EC 3.1.1.3) are hydrolases that catalyze triglycerides hydrolysis in free fatty acids and glycerol. Among the microorganisms that produce lipolytic enzymes, the entophytic fungi stand out. We evaluated 32 fungi of different genera, Pestalotiopsis, Aspergillus, Trichoderma, Penicillium, Fusarium, Colletotrichum, Chaetomium, Mucor, Botryodiplodia, Xylaria, Curvularia, Neocosmospora and Verticillium, isolated from Euterpe oleracea Mart. (Açaizeiro) from the Brazilian Amazon for lipase activity. The presence of lipase was evidenced by the deposition of calcium crystals. The endophytic Pestalotiopsis sp. (31) and Aspergillus sp. (24) with Pz 0.237 (++++) and 0.5 (++++), respectively, were the ones that showed the highest lipolytic activity in a solid medium. Lipase activity was rated in liquid medium, in a different range of temperatures (?C), pH and time (days). The values obtained in the production of lipase by the endophytic fungi were 94% for Pestalotiopsis sp. (31) and 93.87% for Aspergillus sp. (24). Therefore, it is emphasized that the endophytic fungus isolated the E. oleracea palm may be a potential candidate to produce enzymes of global commercial interest.
650 $aMicroorganisms
650 $aEnzima
650 $aVegetal Inferior
700 1 $aFERREIRA, A. M.
700 1 $aMARINHO, V. H.
700 1 $aHOLANDA, F. H. E.
700 1 $aBORGES, S. F.
700 1 $aSOUZA, A. A. de
700 1 $aKOGA, R. de C. R.
700 1 $aLIMA, A. L.
700 1 $aFLORENTINO, A. C.
700 1 $aFERREIRA, I. M.
773 $tMicroorganisms$gv. 10, p. 2394, 2022.
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Embrapa Amapá (CPAF-AP) |
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