Data/hora: 03/06/2024 02:24:54

Biblioteca(s): | Embrapa Amazônia Oriental.
Data corrente: | 02/01/2017
Data da última atualização: | 20/05/2022
Autoria: | MOREIRA, C. M.; ANDRADE, H. B. de; BERTOLUCCI, S. K. V.; LAMEIRA, O. A.; MOHAMMED, A.; PINTO, J. E. B. P.
Afiliação: | Carolina Mariane MOREIRA, UFLA; Helena Botelho de ANDRADE, UFLA; Suzan Kelly Vilela BERTOLUCCI, UFLA; OSMAR ALVES LAMEIRA, CPATU; Aliyu MOHAMMED, Abant ?zzet Baysal University; José Eduardo Brasil Pereira PINTO, UFLA.
Título: | Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species.
Ano de publicação: | 2016
Fonte/Imprenta: | Turkish Journal of Biology, v. 40, n. 6, p. 1227-1234, 2016.
DOI: | 10.3906/biy-1601-56
Idioma: | Inglês
Conteúdo: | The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6- trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%.
Palavras-Chave: | Regeneração in vitro.
Thesagro: | Curaua; Regeneração.
Thesaurus Nal: | thidiazuron.
Categoria do assunto: | G Melhoramento Genético
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/152619/1/Carolina-Moreira-Plantlet-regeneration-curaua-2016.pdf
Registro original: | Embrapa Amazônia Oriental (CPATU)
Exemplares: | CPATU - (53120-1) - UPC - AP - DD -  -  -  - DIS