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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
27/12/2016 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, G. A. da; BERNARDI, T. L.; SCHAKER, P. D. C.; AGUSTINI, B. C.; MELLO, L. M. R. de; VALENTE, P. |
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Afiliação: |
GILDO ALMEIDA DA SILVA, CNPUV; Taís Letícia Bernardi, ²Instituto Federal de Educação Ciência e Tecnologia do Rio Grande do Sul, Sertão, Rio Grande do Sul, Brasil; Patrícia Dayane Carvalho Schaker, mbrapa Uva e Vinho - Laboratório de Microbiologia, Bento Gonçalves, Brazil; BRUNA CARLA AGUSTINI, CNPUV; LOIVA MARIA RIBEIRO DE MELLO, CNPUV; Patrícia Valente, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil. |
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Título: |
Impact of fungicide residues on polymerase chain reaction and on yeast metabolism. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Brazilian Archives of Biology and Technology, v. 59, e16160241, Jan./Dec. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
The indiscriminate use of pesticides on grape crops is harmful for consumers´ healthin “in natura” consumption and in the ingestion of wine and grape juice. During winemaking, a rapid and efficient fermentation stage is critical to avoid proliferation of contaminating microorganisms and to guarantee the product´s quality. Polymerase chain reaction (PCR) has the advantage of detecting these contaminants in the early stages of fermentation. However,this enzymatic reaction may also be susceptible to specific problems, reducing its efficiency. Agricultural practices, such as fungicide treatments, may be a source of PCR inhibiting factors and may also interfere in the normal course of fermentation.The action of the pesticides captan and folpet on PCR and on yeast metabolism was evaluated, once these phthalimide compounds are widely employed in Brazilian vineyards. DNA amplification was only observed at 75 and 37.5 µg/mL of captan concentrations, whereas with folpet, amplification was observed only in the two lowest concentrations tested (42.2 and 21.1µg/mL).Besides the strong inhibition on Taq polymerase activity, phthalimides also inhibited yeast metabolism at all concentrations analyzed.Grape must containing captan and folpet residues could not be transformed into wine due to stuck fermentation caused by the inhibition of yeast metabolism. Noncompliance with the waiting period for phthalimide fungicides may result in financial liabilities to the viticulture sector.The use of yeasts with high fungicide sensitivity should be selected for must fermentation as a strategy for sustainable wine production and to assure that products comply with health and food safety standards. Key words: fungicide;PCR inhibitors; stuck fermentation;Taq polymerase inhibitors; yeast growth MenosThe indiscriminate use of pesticides on grape crops is harmful for consumers´ healthin “in natura” consumption and in the ingestion of wine and grape juice. During winemaking, a rapid and efficient fermentation stage is critical to avoid proliferation of contaminating microorganisms and to guarantee the product´s quality. Polymerase chain reaction (PCR) has the advantage of detecting these contaminants in the early stages of fermentation. However,this enzymatic reaction may also be susceptible to specific problems, reducing its efficiency. Agricultural practices, such as fungicide treatments, may be a source of PCR inhibiting factors and may also interfere in the normal course of fermentation.The action of the pesticides captan and folpet on PCR and on yeast metabolism was evaluated, once these phthalimide compounds are widely employed in Brazilian vineyards. DNA amplification was only observed at 75 and 37.5 µg/mL of captan concentrations, whereas with folpet, amplification was observed only in the two lowest concentrations tested (42.2 and 21.1µg/mL).Besides the strong inhibition on Taq polymerase activity, phthalimides also inhibited yeast metabolism at all concentrations analyzed.Grape must containing captan and folpet residues could not be transformed into wine due to stuck fermentation caused by the inhibition of yeast metabolism. Noncompliance with the waiting period for phthalimide fungicides may result in financial liabilities to the viticulture sector.The use of ye... Mostrar Tudo |
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Palavras-Chave: |
Fungicide; PCR inhibitors; Stuck fermentation; Taq polymerase inhibitors. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/152340/1/Gildo-1678-4324-babt-1678-4324-2016160241.pdf
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Marc: |
LEADER 02505naa a2200229 a 4500
001 2059337
005 2019-05-06
008 2016 bl uuuu u00u1 u #d
100 1 $aSILVA, G. A. da
245 $aImpact of fungicide residues on polymerase chain reaction and on yeast metabolism.$h[electronic resource]
260 $c2016
520 $aThe indiscriminate use of pesticides on grape crops is harmful for consumers´ healthin “in natura” consumption and in the ingestion of wine and grape juice. During winemaking, a rapid and efficient fermentation stage is critical to avoid proliferation of contaminating microorganisms and to guarantee the product´s quality. Polymerase chain reaction (PCR) has the advantage of detecting these contaminants in the early stages of fermentation. However,this enzymatic reaction may also be susceptible to specific problems, reducing its efficiency. Agricultural practices, such as fungicide treatments, may be a source of PCR inhibiting factors and may also interfere in the normal course of fermentation.The action of the pesticides captan and folpet on PCR and on yeast metabolism was evaluated, once these phthalimide compounds are widely employed in Brazilian vineyards. DNA amplification was only observed at 75 and 37.5 µg/mL of captan concentrations, whereas with folpet, amplification was observed only in the two lowest concentrations tested (42.2 and 21.1µg/mL).Besides the strong inhibition on Taq polymerase activity, phthalimides also inhibited yeast metabolism at all concentrations analyzed.Grape must containing captan and folpet residues could not be transformed into wine due to stuck fermentation caused by the inhibition of yeast metabolism. Noncompliance with the waiting period for phthalimide fungicides may result in financial liabilities to the viticulture sector.The use of yeasts with high fungicide sensitivity should be selected for must fermentation as a strategy for sustainable wine production and to assure that products comply with health and food safety standards. Key words: fungicide;PCR inhibitors; stuck fermentation;Taq polymerase inhibitors; yeast growth
653 $aFungicide
653 $aPCR inhibitors
653 $aStuck fermentation
653 $aTaq polymerase inhibitors
700 1 $aBERNARDI, T. L.
700 1 $aSCHAKER, P. D. C.
700 1 $aAGUSTINI, B. C.
700 1 $aMELLO, L. M. R. de
700 1 $aVALENTE, P.
773 $tBrazilian Archives of Biology and Technology$gv. 59, e16160241, Jan./Dec. 2016.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 22 | |
| 4. |  | BERNARDI, T. L.; SCHAKER, P. D. C.; TODESCHINI, A. P.; VALENTE, P.; SILVA, G. A. da. Ação do ácido gálico e da catequina na inibição da Reação em Cadeira da Polimerase. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 8.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 4., 2010, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2010. p. 23. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 9. | | BERNARDI, T. L.; SCHAKER, P. D. C.; TODESCHINI, A. P.; VALENTE, P.; SILVA, G. A. da. Uso da enzima Tth DNA polimerase para amplificação direta de DNA de leveduras contido em vinho. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 8.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 4., 2010, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2010. p. p. 38. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 12. | | SILVA, G. A. da; BERNARDI, T. L.; SCHAKER, P. D. C.; MENEGOTTO, M.; VALENTE, P. Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification. Brazilian Archives of Biology and Technology, Curitiba, v. 55, n. 2, p. 319-327, mar./abr. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 13. | | NALIN, R.; AUGUSTINI, B. C.; VALENTE, P.; BERNARDI, T. L.; SILVA, G. A. da. Primer universal para leveduras não detecta a presença de Bacillus megaterium. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 9.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 5., 2011, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2011. p. 19. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 17. | | BERNARDI, T. L.; SCHAKER, P. D. C.; MENEGOTTO, M.; VALENTE, P.; SILVA, G. A. da. Inibição da reação em cadeia da polimerase por taninos do vinho. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 7.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 3., 2009, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2009. p. 53.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 19. | | SILVA, G. A. da; MENEGOTTO, M.; SCHAKER, P.; BERNARDI, T. L.; MELLO, L. M. R. de; PEREIRA, G. E. São Francisco Valley Region and the autochthonous yeasts of the Brazilian Northeast. In: WORLD CONGRESS OF VINE AND WINE, 34.; GENERAL ASSEMBLY OF OIV, 9., 2011, Porto. The Construction of wine: conspiracy of knowledge and art: proceedings. Lisboa: Um Porto para o Mundo, 2011. Não paginado. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 20. | | SILVA, G. A. da; MENEGOTTO, M.; SCHAKER, P.; BERNARDI, T. L.; MELLO, L. M. R. de; PEREIRA, G. E. São Francisco Valley Region and the autochthonous yeasts of the Brazilian Northeast. In: WORLD CONGRESS OF VINE AND WINE, 34.; GENERAL ASSEMBLY OF OIV, 9., 2011, Porto. The Construction of wine: conspiracy of knowledge and art: proceedings. Lisboa: Um Porto para o Mundo, 2011. Não paginado. 1 CD-ROM. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 22 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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