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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
05/08/2011 |
Data da última atualização: |
05/08/2011 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ANDREOTTI, R.; PÉREZ DE LEÓN, A. A.; DOWD, S. E.; GUERRERO, F. D.; BENDELE, K. G.; SCOLES, G. A. |
Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; Adalberto A Pérez de León, 2USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Rd., Kerrville, TX, 78028, USA; Scot E Dowd, 3Research and Testing Laboratory, Pathogenius, and Spirostat Technologies, 4321 Marsha Sharp Fwy., Lubbock, TX, 79407, USA; Felix D Guerrero, 2USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Rd., Kerrville, TX, 78028, USA.; Kylie G Bendele, 2USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Rd., Kerrville, TX, 78028, USA.; Glen A Scoles, 4USDA-ARS Animal Disease Research Unit, Washington State University, 3003 ADBF, Pullman, WA, 99164, USA. |
Título: |
Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
BMC Microbiology, v.11, p. 1-11, 2011. |
Idioma: |
Inglês |
Conteúdo: |
Ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The cattle tick, Rhipicephalus (Boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. Tick microbiomes remain largely unexplored. The objective of this study was to explore the R. microplus microbiome by applying the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to characterize its bacterial diversity. Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas. Raw data from bTEFAP were screened and trimmed based upon quality scores and binned into individual sample collections. Bacteria identified to the species level include Staphylococcus aureus, Staphylococcus chromogenes, Streptococcus dysgalactiae, Staphylococcus sciuri, Serratia marcescens, Corynebacterium glutamicum, and Finegoldia magna. One hundred twenty-one bacterial genera were detected in all the life stages and tissues sampled. The total number of genera identified by tick sample comprised: 53 in adult males, 61 in adult females, 11 in gut tissue, 7 in ovarian tissue, and 54 in the eggs. Notable genera detected in the cattle tick include Wolbachia, Coxiella, and Borrelia. The molecular approach applied in this study allowed us to assess the relative abundance of the microbiota associated with R. microplus. This report represents the first survey of the bacteriome in the cattle tick using non-culture based molecular approaches. Comparisons of our results with previous bacterial surveys provide an indication of geographic variation in the assemblages of bacteria associated with R. microplus. Additional reports on the identification of new bacterial species maintained in nature by R. microplus that may be pathogenic to its vertebrate hosts are expected as our understanding of its microbiota expands. Increased awareness of the role R. microplus can play in the transmission of pathogenic bacteria will enhance our ability to mitigate its economic impact on animal agriculture globally. This recognition should be included as part of analyses to assess the risk for re-invasion of areas like the United States of America where R. microplus was eradicated. MenosTicks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The cattle tick, Rhipicephalus (Boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. Tick microbiomes remain largely unexplored. The objective of this study was to explore the R. microplus microbiome by applying the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to characterize its bacterial diversity. Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas. Raw data from bTEFAP were screened and trimmed based upon quality scores and binned into individual sample collections. Bacteria identified to the species level include Staphylococcus aureus, Staphylococcus chromogenes, Streptococcus dysgalactiae, Staphylococcus sciuri, Serratia marcescens, Corynebacterium glutamicum, and Finegoldia magna. One hundred twenty-one bacterial genera were detected in all the life stages and tissues sampled. The total number of genera identified by tick sample comprised: 53 in adult males, 61 in adult females, 11 in gut tissue, 7 in ovarian tissue, and 54 in the eggs. Notable genera detected in the cattle tick include Wolbachia, Coxiella, and Borrelia. The molecular approach applied in this study allowed us to assess the relative abundance of the microbiota ... Mostrar Tudo |
Thesagro: |
Boophilus Microplus; Carrapato; Sanidade Animal. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/39345/1/bmc-microbiology.pdf
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Marc: |
LEADER 03046naa a2200217 a 4500 001 1897418 005 2011-08-05 008 2011 bl uuuu u00u1 u #d 100 1 $aANDREOTTI, R. 245 $aAssessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing.$h[electronic resource] 260 $c2011 520 $aTicks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. The cattle tick, Rhipicephalus (Boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. Tick microbiomes remain largely unexplored. The objective of this study was to explore the R. microplus microbiome by applying the bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) technique to characterize its bacterial diversity. Pyrosequencing was performed on adult males and females, eggs, and gut and ovary tissues from adult females derived from samples of R. microplus collected during outbreaks in southern Texas. Raw data from bTEFAP were screened and trimmed based upon quality scores and binned into individual sample collections. Bacteria identified to the species level include Staphylococcus aureus, Staphylococcus chromogenes, Streptococcus dysgalactiae, Staphylococcus sciuri, Serratia marcescens, Corynebacterium glutamicum, and Finegoldia magna. One hundred twenty-one bacterial genera were detected in all the life stages and tissues sampled. The total number of genera identified by tick sample comprised: 53 in adult males, 61 in adult females, 11 in gut tissue, 7 in ovarian tissue, and 54 in the eggs. Notable genera detected in the cattle tick include Wolbachia, Coxiella, and Borrelia. The molecular approach applied in this study allowed us to assess the relative abundance of the microbiota associated with R. microplus. This report represents the first survey of the bacteriome in the cattle tick using non-culture based molecular approaches. Comparisons of our results with previous bacterial surveys provide an indication of geographic variation in the assemblages of bacteria associated with R. microplus. Additional reports on the identification of new bacterial species maintained in nature by R. microplus that may be pathogenic to its vertebrate hosts are expected as our understanding of its microbiota expands. Increased awareness of the role R. microplus can play in the transmission of pathogenic bacteria will enhance our ability to mitigate its economic impact on animal agriculture globally. This recognition should be included as part of analyses to assess the risk for re-invasion of areas like the United States of America where R. microplus was eradicated. 650 $aBoophilus Microplus 650 $aCarrapato 650 $aSanidade Animal 700 1 $aPÉREZ DE LEÓN, A. A. 700 1 $aDOWD, S. E. 700 1 $aGUERRERO, F. D. 700 1 $aBENDELE, K. G. 700 1 $aSCOLES, G. A. 773 $tBMC Microbiology$gv.11, p. 1-11, 2011.
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Embrapa Gado de Corte (CNPGC) |
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Biblioteca(s): |
Embrapa Acre. |
Data corrente: |
06/09/2017 |
Data da última atualização: |
16/11/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
OLIVEIRA, J. C. de; RUFINO, P. B.; AZÊVEDO, H. S. da S.; SILVA, L. M. da; ASSIS, G. M. L. de; CAMPOS, T. de. |
Afiliação: |
Jônatas Chagas de Oliveira, Universidade Federal do Acre (Ufac); Polinar Bandeira Rufino, Fameta; Hellen Sandra Freires da Silva Azêvedo, Universidade Federal do Acre (Ufac); LUCIELIO MANOEL DA SILVA, CPAF-Acre; GISELLE MARIANO LESSA DE ASSIS, CPAF-Acre; TATIANA DE CAMPOS, CPAF-Acre. |
Título: |
Diversidade genética de acessos do banco de germoplasma de amendoim forrageiro. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: CONGRESSO REGIONAL DE PESQUISA DO ESTADO DO ACRE, 2.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFAC, 25., 2016, Rio Branco/Cruzeiro do Sul. Inovação: sustentabilidade e desenvolvimento regional: anais. Rio Branco, AC: Edufac, 2016. |
Páginas: |
p. 103. |
ISBN: |
978-85-8236-050-7 |
Idioma: |
Português |
Conteúdo: |
O amendoim forrageiro (Arachis pintoi) é uma leguminosa pertencente à família Fabaceae e tem sido utilizado em consorciação de pastagens, agregando as mesmas alto valor nutritivo e produção de forragem, além da fixação biológica de nitrogênio, reduzindo os custos do produtor. O objetivo do estudo foi analisar a diversidade genética de genótipos de amendoim forrageiro por meio de marcadores microssatélites (SSR). |
Palavras-Chave: |
Acre; Amazonia Occidental; Amazônia Ocidental; Amendoim forrageiro; Cacauhetes forrajeros; Embrapa Acre; Fitomejoramiento; Forage peanuts; Leguminosas forrajeras; Marcador microssatélite; Repeticiones de microsatélite; Rio Branco (AC); Variación genética; Western Amazon. |
Thesagro: |
Banco de Germoplasma; Leguminosa forrageira; Marcador genético; Melhoramento genético vegetal; Variação genética. |
Thesaurus NAL: |
Arachis pintoi; Forage legumes; Genetic variation; Microsatellite repeats; Plant breeding. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163547/1/26369.pdf
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Marc: |
LEADER 02047nam a2200481 a 4500 001 2075144 005 2023-11-16 008 2016 bl uuuu u00u1 u #d 020 $a978-85-8236-050-7 100 1 $aOLIVEIRA, J. C. de 245 $aDiversidade genética de acessos do banco de germoplasma de amendoim forrageiro.$h[electronic resource] 260 $aIn: CONGRESSO REGIONAL DE PESQUISA DO ESTADO DO ACRE, 2.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFAC, 25., 2016, Rio Branco/Cruzeiro do Sul. Inovação: sustentabilidade e desenvolvimento regional: anais. Rio Branco, AC: Edufac$c2016 300 $ap. 103. 520 $aO amendoim forrageiro (Arachis pintoi) é uma leguminosa pertencente à família Fabaceae e tem sido utilizado em consorciação de pastagens, agregando as mesmas alto valor nutritivo e produção de forragem, além da fixação biológica de nitrogênio, reduzindo os custos do produtor. O objetivo do estudo foi analisar a diversidade genética de genótipos de amendoim forrageiro por meio de marcadores microssatélites (SSR). 650 $aArachis pintoi 650 $aForage legumes 650 $aGenetic variation 650 $aMicrosatellite repeats 650 $aPlant breeding 650 $aBanco de Germoplasma 650 $aLeguminosa forrageira 650 $aMarcador genético 650 $aMelhoramento genético vegetal 650 $aVariação genética 653 $aAcre 653 $aAmazonia Occidental 653 $aAmazônia Ocidental 653 $aAmendoim forrageiro 653 $aCacauhetes forrajeros 653 $aEmbrapa Acre 653 $aFitomejoramiento 653 $aForage peanuts 653 $aLeguminosas forrajeras 653 $aMarcador microssatélite 653 $aRepeticiones de microsatélite 653 $aRio Branco (AC) 653 $aVariación genética 653 $aWestern Amazon 700 1 $aRUFINO, P. B. 700 1 $aAZÊVEDO, H. S. da S. 700 1 $aSILVA, L. M. da 700 1 $aASSIS, G. M. L. de 700 1 $aCAMPOS, T. de
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