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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
21/03/2022 |
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Data da última atualização: |
22/03/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GALLENTI, R.; HUSSEIN, H. E.; ALZAN, H. F.; SUAREZ, C. E.; UETI, M.; ASURMENDI, S.; BENITEZ, D.; ARAUJO, F. R.; ROLLS, P.; SIBEKO-MATJILA, K.; SCHNITTGER, L.; FLORIN-CHRISTENSEN, M. |
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Afiliação: |
ROMINA GALLENTI, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; HALA E. HUSSEIN, Washington State University, USA.; HEBA F. ALZAN, Washington State University, USA.; CARLOS E. SUAREZ, Washington State University, USA.; MASSARO UETI, Washington State University, USA.; SEBASTIÁN ASURMENDI, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; DANIEL BENITEZ, Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina.; FLABIO RIBEIRO DE ARAUJO, CNPGC; PETER ROLLS, Department of Agriculture & Fisheries, Tick Fever Centre, Australia.; KGOMOTSO SIBEKO-MATJILA, University of Pretoria, South Africa.; LEONHARD SCHNITTGER, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina.; MÓNICA FLORIN-CHRISTENSEN, Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas (INTA-CONICET), Argentina. |
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Título: |
Unraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Pathogens, v. 11, n. 344, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Babesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host?pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen. |
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Palavras-Chave: |
Inter-strain polymorphism; Rhomboid serine proteases. |
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Thesagro: |
Babesia Bovis. |
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Thesaurus Nal: |
Babesiosis; Gene expression; Tick-borne diseases. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232800/1/2022-Babesia-ROM4.pdf
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Marc: |
LEADER 02337naa a2200325 a 4500
001 2141112
005 2022-03-22
008 2022 bl uuuu u00u1 u #d
100 1 $aGALLENTI, R.
245 $aUnraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies.$h[electronic resource]
260 $c2022
520 $aBabesia bovis, a tick-transmitted apicomplexan protozoon, infects cattle in tropical and subtropical regions around the world. In the apicomplexans Toxoplasma gondii and Plasmodium falciparum, rhomboid serine protease 4 (ROM4) fulfills an essential role in host cell invasion. We thus investigated B. bovis ROM4 coding genes; their genomic organization; their expression in in vitro cultured asexual (AS) and sexual stages (SS); and strain polymorphisms. B. bovis contains five rom4 paralogous genes in chromosome 2, which we have named rom4.1, 4.2, 4.3, 4.4 and 4.5. There are moderate degrees of sequence identity between them, except for rom4.3 and 4.4, which are almost identical. RT-qPCR analysis showed that rom4.1 and rom4.3/4.4, respectively, display 18-fold and 218-fold significantly higher (p < 0.01) levels of transcription in SS than in AS, suggesting a role in gametogenesis-related processes. In contrast, transcription of rom4.4 and 4.5 differed non-significantly between the stages. ROM4 polymorphisms among geographic isolates were essentially restricted to the number of tandem repeats of a 29-amino acid sequence in ROM4.5. This sequence repeat is highly conserved and predicted as antigenic. B. bovis ROMs likely participate in relevant host?pathogen interactions and are possibly useful targets for the development of new control strategies against this pathogen.
650 $aBabesiosis
650 $aGene expression
650 $aTick-borne diseases
650 $aBabesia Bovis
653 $aInter-strain polymorphism
653 $aRhomboid serine proteases
700 1 $aHUSSEIN, H. E.
700 1 $aALZAN, H. F.
700 1 $aSUAREZ, C. E.
700 1 $aUETI, M.
700 1 $aASURMENDI, S.
700 1 $aBENITEZ, D.
700 1 $aARAUJO, F. R.
700 1 $aROLLS, P.
700 1 $aSIBEKO-MATJILA, K.
700 1 $aSCHNITTGER, L.
700 1 $aFLORIN-CHRISTENSEN, M.
773 $tPathogens$gv. 11, n. 344, 2022.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 7 | |
| 1. |  | FRAISSE, C.; PERONDI, D.; KARREI, M.; FARIAS, J. R. B.; BENITEZ, D. Seguro paramétrico como nova ferramenta para gerenciar o risco climático na cultura da soja. In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. Regina Maria Villas Bôas de Campos Leite, Adeney de Freitas Bueno, editores técnicos. resumo 266. p. 291.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 3. | | FLORES, D. A.; MINICHIELLO, Y.; ARAUJO, F. R.; SHKAP, V.; BENITEZ, D.; ECHAIDE, I.; ROLLS, P.; MOSQUEDA, J.; PACHECO, G. M.; PETTERSON, M.; FLORIN-CHRISTENSEN, M.; SCHNITTGER, L. Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation Transboundary and Emerging Diseases, v. 60, p. 131-136, Nov. 2013. Supplement 2.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | GALLENTI, R.; HUSSEIN, H. E.; ALZAN, H. F.; SUAREZ, C. E.; UETI, M.; ASURMENDI, S.; BENITEZ, D.; ARAUJO, F. R.; ROLLS, P.; SIBEKO-MATJILA, K.; SCHNITTGER, L.; FLORIN-CHRISTENSEN, M. Unraveling the complexity of the Rhomboid Serine Protease 4 Family of Babesia bovis using bioinformatics and experimental studies. Pathogens, v. 11, n. 344, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | DELGADO, J. V.; RUMIANO, F.; VACA, J. L.; CARDOZO, A.; SERENO, J. R. B.; BENITEZ, D.; MARIANTE, A. S.; ANZOLA, H.; VELAZQUEZ, F.; CAMACHO, M. E.; SIERRA, A.; HERNANDEZ, J. S.; PEREZGROVAS, R.; MEDRANO, A.; ALUJA, A.; GOMEZ URVIOLA, N.; CARCAMO, F.; FERNANDEZ, G.; PARIACOTE, F.; TOLEDO, P. M. Balance de cinco anos en pro de la conservacion de razas Iberoamericanas: Red Cyted-XII-H. In: CONGRESO IBEROAMERICANO DE RAZAS CRIOLLAS, 6.; SIMPOSIO IBEROAMERICANO SOBRE CONSERVACION Y UTILIZACION DE RECURSOS ZOOGENETICOS, 4., 2003, Recife. Resumenes de Ponencias y Comunicaciones. Recife: FIRC / CYTED, 2003. nao paginado.| Biblioteca(s): Embrapa Pantanal. |
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| 6. | | DELGADO, J. V.; MARTINEZ, R.; REVIDATTI, M. A.; VACA, J. L.; STEMMER, A.; SERENO, J. R. B.; BENITEZ, D.; MARIANTE, A. S.; RIBEIRO, M. N.; RIBAMAR, J.; ANZOLA, H.; VELAZQUEZ, F.; ZAMBRANO, D.; CAMACHO, M. E.; SIERRA, A.; HERNANDEZ, J. S.; PEREZGROVAS, R.; MEDRANO, A.; ALUJA, A.; GOMEZ URVIOLA, N.; MATOS, C.; CARCAMO, F.; FERNÁNDEZ, G.; PIRIACOTE, F.; TOLEDO, P. M. Balance de siete años en pro de la conservación de razas iberoamericanas: red cyted-XII-H. Archivos de Zootecnia, v. 54, n. 206-207, p. 129-134, 2005.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - C |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | BERMEJO, J. V. D.; MARTÍNEZ, R.; REVIDATTI, M. A.; VACA, J. L.; STEMMER, A.; SERENO, J. R. B.; BENÍTEZ, D.; MARIANTE, A. S.; RIBEIRO, M. N.; RIBAMAR, J.; ANZOLA, H.; VELAZQUEZ, F.; ZAMBRANO, D.; CAMACHO, M. E.; SIERRA, A.; HERNANDEZ, J. S.; PEREZGROVAS, R.; MEDRANO, A.; ALUJA, A.; GÓMEZ URVIOLA, N.; MATOS, C.; CÁRCAMO, F.; FERNÁNDEZ, G.; PARIACOTE, F. Red CYTED-XII-H: haciendo iberoamérica desde la cooperación científica para la conservación y uso de los recursos zoogenéticos. In: SIMPÓSIO IBEROAMERICANO SOBRE CONSERVACIÓN Y UTILIZACIÓN DE RECURSOS ZOOGENÉTICOS, 6., 2005, Chiapas, México. Anais. Chiapas, México: CYTED: Universidad Autónoma de Chiapas, 2005. p.2-8.| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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