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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Hortaliças. |
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Data corrente: |
05/08/2004 |
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Data da última atualização: |
05/08/2004 |
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Autoria: |
BRINGEL, J. M.; BEDENDO, I. P.; GUIMARÃES, P. M.; LOPES, C. A. |
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Afiliação: |
Embrapa Hortaliças, Brasília, DF. |
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Título: |
Genetic diversity of Ralstonia solanacearum biovar 2 isolates of potato and eggplant from Brazil by PCR. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Summa Phytopathologica, Piracicaba, v. 30, n. 3, p. 350-355, jul./set. 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
The bacterial wilt disease caused by Ralstonia solanacearum affects mainly solanaceous species such as potato, eggplant, peppers, tomato and Brazilian gilo (Solanum gilo). The molecular characterization of R. solanacearum biovar 2 as the main objective of this study. Fifty-one biovar 2 isolates were studied (9 eggplant isolates and 42 potato isolates), all of them collected in different regions of Brazil. According to molecular analysis the isolates were clustered in four different groups with distinct banding patterns with the primers BOX and ERIC, and five groups with the primers REP. The identification of potato isolates as the biovar 2-A, and the and eggplant isolates as biovar 2-T were based on biochemical tests using trehalose and was confirmed by molecular analyses. Interesting results, based on molecular data, were observed when the groups of isolates were associated with the geographical region from when they were collected. Group I clustered only the isolates collected in Paraná State. Group II clustered the isolates collected in the State of Bahia, Paraná and Brasília-DF. Group III clustered all isolates of eggplants and only one of potatoes, all of which were collected in Brasília-DF. Group IV, like group II, clustered isolates from different States (Paraná, Goiás, Rio Grande do Sul and Brasília-DF). These results suggest a relationship between the isolates clustered through molecular analysis in groups I and III and the geographical region where they were collected. In contrast, for both groups II and IV, isolates with similar genetic background were collected from different regions. MenosThe bacterial wilt disease caused by Ralstonia solanacearum affects mainly solanaceous species such as potato, eggplant, peppers, tomato and Brazilian gilo (Solanum gilo). The molecular characterization of R. solanacearum biovar 2 as the main objective of this study. Fifty-one biovar 2 isolates were studied (9 eggplant isolates and 42 potato isolates), all of them collected in different regions of Brazil. According to molecular analysis the isolates were clustered in four different groups with distinct banding patterns with the primers BOX and ERIC, and five groups with the primers REP. The identification of potato isolates as the biovar 2-A, and the and eggplant isolates as biovar 2-T were based on biochemical tests using trehalose and was confirmed by molecular analyses. Interesting results, based on molecular data, were observed when the groups of isolates were associated with the geographical region from when they were collected. Group I clustered only the isolates collected in Paraná State. Group II clustered the isolates collected in the State of Bahia, Paraná and Brasília-DF. Group III clustered all isolates of eggplants and only one of potatoes, all of which were collected in Brasília-DF. Group IV, like group II, clustered isolates from different States (Paraná, Goiás, Rio Grande do Sul and Brasília-DF). These results suggest a relationship between the isolates clustered through molecular analysis in groups I and III and the geographical region where they were collec... Mostrar Tudo |
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Palavras-Chave: |
Beringela; Biovar 2; Diversidade genética; Murcha; PCR; Solanáceas. |
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Thesagro: |
Agricultura; Batata; Berinjela; Doença de Planta; Doença Vascular; Murcha Bacteriana; Ralstonia Solanacearum; Solanum Melongena. |
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Categoria do assunto: |
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Marc: |
LEADER 02540naa a2200325 a 4500
001 1776510
005 2004-08-05
008 2004 bl uuuu u00u1 u #d
100 1 $aBRINGEL, J. M.
245 $aGenetic diversity of Ralstonia solanacearum biovar 2 isolates of potato and eggplant from Brazil by PCR.
260 $c2004
520 $aThe bacterial wilt disease caused by Ralstonia solanacearum affects mainly solanaceous species such as potato, eggplant, peppers, tomato and Brazilian gilo (Solanum gilo). The molecular characterization of R. solanacearum biovar 2 as the main objective of this study. Fifty-one biovar 2 isolates were studied (9 eggplant isolates and 42 potato isolates), all of them collected in different regions of Brazil. According to molecular analysis the isolates were clustered in four different groups with distinct banding patterns with the primers BOX and ERIC, and five groups with the primers REP. The identification of potato isolates as the biovar 2-A, and the and eggplant isolates as biovar 2-T were based on biochemical tests using trehalose and was confirmed by molecular analyses. Interesting results, based on molecular data, were observed when the groups of isolates were associated with the geographical region from when they were collected. Group I clustered only the isolates collected in Paraná State. Group II clustered the isolates collected in the State of Bahia, Paraná and Brasília-DF. Group III clustered all isolates of eggplants and only one of potatoes, all of which were collected in Brasília-DF. Group IV, like group II, clustered isolates from different States (Paraná, Goiás, Rio Grande do Sul and Brasília-DF). These results suggest a relationship between the isolates clustered through molecular analysis in groups I and III and the geographical region where they were collected. In contrast, for both groups II and IV, isolates with similar genetic background were collected from different regions.
650 $aAgricultura
650 $aBatata
650 $aBerinjela
650 $aDoença de Planta
650 $aDoença Vascular
650 $aMurcha Bacteriana
650 $aRalstonia Solanacearum
650 $aSolanum Melongena
653 $aBeringela
653 $aBiovar 2
653 $aDiversidade genética
653 $aMurcha
653 $aPCR
653 $aSolanáceas
700 1 $aBEDENDO, I. P.
700 1 $aGUIMARÃES, P. M.
700 1 $aLOPES, C. A.
773 $tSumma Phytopathologica, Piracicaba$gv. 30, n. 3, p. 350-355, jul./set. 2004.
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Embrapa Hortaliças (CNPH) |
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