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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
13/02/2009 |
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Data da última atualização: |
14/11/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, D. C. G.; MORTEL, M. van de; LEMOS, N. G.; STOLF, R.; ALMEIDA, A. M. R.; NEPOMUCENO, A. L.; YAMANAKA, N.; BAUM, T. J.; WHITHAM, S. A.; ABDELNOOR, R. |
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Afiliação: |
Danielle C.G. Silva, Danielle C. G. Silva, CNPSo / FFALM / UNESP, Jaboticabal; Martjin van de Mortel, Iowa State University ISU, Department of Plant Pathology; Noelle G. Lemos, CNPSo; Renata Stolf, CNPSo / FFALM; Alvaro M. R. Almeida, CNPSo; Alexandre L. |
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Título: |
Characterization of EST libraries from soybean plants involved in resistant and susceptible interactions with the asian soybean rust pathogen. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. [S.l.], 2008, p. 220, P405. |
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Idioma: |
Inglês |
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Conteúdo: |
Asian rust, caused by Phakopsora pachyrhizi, is one of the most important soybean diseases. Despite of its importance, little is known about the molecular mechanism of interaction between the soybean plant and this pathogen. The aim of this study was to analyze four suppression subtractive hybridization (SSH) cDNA libraries of soybean under rust infection, in order to identify transcripts involved in soybean rust resistance. Leaf samples of the resistant soybean line PI230970 and the susceptible cultivar Embrapa 48 were collected at 24 and 192 hours after inoculation. Using SSH we generated four libraries: P24 and P192 (from PI230970), E24 and E192 (from Embrapa 48). A total of 4,704 clones were obtained from these four libraries, consisting of 1,456 clones from P24 library, 960 clones from P192 library, 2,000 clones from E24 library, and 672 clones from E192 library. The libraries were sequenced and compared to GenBank non-redundant (nr/nt) database using the tBLASTX algorithm. More than 90% of the sequences had good sequence quality (Phred>20) and 1,281 (27.2%) clones were unique sequences, resulting in an average redundancy of 71.4%. The automatic functional categorization by Gene Ontology over the non-redundant libraries revealed the biological processes response to other organisms, glycolysis, defense response, and systemic acquired resistance as the most representative. These results will contribute to the comprehension of the resistance mechanisms of soybean plants to this pathogen, and can assist the development of more efficient methods to control this disease. MenosAsian rust, caused by Phakopsora pachyrhizi, is one of the most important soybean diseases. Despite of its importance, little is known about the molecular mechanism of interaction between the soybean plant and this pathogen. The aim of this study was to analyze four suppression subtractive hybridization (SSH) cDNA libraries of soybean under rust infection, in order to identify transcripts involved in soybean rust resistance. Leaf samples of the resistant soybean line PI230970 and the susceptible cultivar Embrapa 48 were collected at 24 and 192 hours after inoculation. Using SSH we generated four libraries: P24 and P192 (from PI230970), E24 and E192 (from Embrapa 48). A total of 4,704 clones were obtained from these four libraries, consisting of 1,456 clones from P24 library, 960 clones from P192 library, 2,000 clones from E24 library, and 672 clones from E192 library. The libraries were sequenced and compared to GenBank non-redundant (nr/nt) database using the tBLASTX algorithm. More than 90% of the sequences had good sequence quality (Phred>20) and 1,281 (27.2%) clones were unique sequences, resulting in an average redundancy of 71.4%. The automatic functional categorization by Gene Ontology over the non-redundant libraries revealed the biological processes response to other organisms, glycolysis, defense response, and systemic acquired resistance as the most representative. These results will contribute to the comprehension of the resistance mechanisms of soybean plants to... Mostrar Tudo |
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Palavras-Chave: |
Characterization of EST libraries from soybean plants involved in resistant and susceptible interactions with the asian. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/46470/1/28856.pdf
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Marc: |
LEADER 02516nam a2200229 a 4500
001 1471145
005 2011-11-14
008 2008 bl uuuu u00u1 u #d
100 1 $aSILVA, D. C. G.
245 $aCharacterization of EST libraries from soybean plants involved in resistant and susceptible interactions with the asian soybean rust pathogen.
260 $aIn: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. [S.l.], 2008, p. 220, P405.$c2008
520 $aAsian rust, caused by Phakopsora pachyrhizi, is one of the most important soybean diseases. Despite of its importance, little is known about the molecular mechanism of interaction between the soybean plant and this pathogen. The aim of this study was to analyze four suppression subtractive hybridization (SSH) cDNA libraries of soybean under rust infection, in order to identify transcripts involved in soybean rust resistance. Leaf samples of the resistant soybean line PI230970 and the susceptible cultivar Embrapa 48 were collected at 24 and 192 hours after inoculation. Using SSH we generated four libraries: P24 and P192 (from PI230970), E24 and E192 (from Embrapa 48). A total of 4,704 clones were obtained from these four libraries, consisting of 1,456 clones from P24 library, 960 clones from P192 library, 2,000 clones from E24 library, and 672 clones from E192 library. The libraries were sequenced and compared to GenBank non-redundant (nr/nt) database using the tBLASTX algorithm. More than 90% of the sequences had good sequence quality (Phred>20) and 1,281 (27.2%) clones were unique sequences, resulting in an average redundancy of 71.4%. The automatic functional categorization by Gene Ontology over the non-redundant libraries revealed the biological processes response to other organisms, glycolysis, defense response, and systemic acquired resistance as the most representative. These results will contribute to the comprehension of the resistance mechanisms of soybean plants to this pathogen, and can assist the development of more efficient methods to control this disease.
653 $aCharacterization of EST libraries from soybean plants involved in resistant and susceptible interactions with the asian
700 1 $aMORTEL, M. van de
700 1 $aLEMOS, N. G.
700 1 $aSTOLF, R.
700 1 $aALMEIDA, A. M. R.
700 1 $aNEPOMUCENO, A. L.
700 1 $aYAMANAKA, N.
700 1 $aBAUM, T. J.
700 1 $aWHITHAM, S. A.
700 1 $aABDELNOOR, R.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 3 | |
| 2. |  | DUENHAS, L. H.; OLIVEIRA, M. V. M. de; LOPES, M. D. C.; GOMES, E. P.; DALRI, A. B. Avaliação nutricional de macronutrientes em pomar fertirrigado de laranja Valência utilizando o DRIS-CITROS. In: CONGRESSO BRASILEIRO DE FERTIRRIGFAÇÃO, 1.; MOSTRA DE EQUIPAMENTOS E PRODUTOS UTILIZADOS NA IRRIGAÇÃO E FERTIRRIGAÇÃO, 1., 2003, João Pessoa. Anais... João Pessoa : UFPB; Embrapa Semi-Árido; Areia: CCA, 2003. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 3. | | DUENHAS, L. H.; VILLAS BÔAS, R. L.; SOUZA, C. M. P.; OLIVEIRA, M. V. A. M.; DALRI, A. B. Produção, qualidade dos frutos e estado nutricional da laranja Valência sob fertirrigação e adubação convencional. Engenharia Agrícola, Jaboticabal, v. 25, n. 1, p. 154-160, jan./abr. 2005.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 3 | |
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