Data/hora: 06/06/2024 14:54:00

Biblioteca(s): | Embrapa Soja.
Data corrente: | 15/09/2010
Data da última atualização: | 03/08/2017
Autoria: | BATISTA, J. S. DA S.; TORRES, A. R.; HUNGRIA, M.
Afiliação: | JESIANE STEFÂNIA DA SILVA BATISTA, UEL; ADALGISA RIBEIRO TORRES; MARIANGELA HUNGRIA DA CUNHA, CNPSO.
Título: | Towards a two-dimensional proteomic reference map of Bradyrhizobium japonicum CPAC 15: Spotlighting "hypothetical proteins".
Ano de publicação: | 2010
Fonte/Imprenta: | Proteomics, Weinheim, v. 10, n. 17, p. 3176-3189, Sept. 2010.
ISSN: | 1615-9861
DOI: | DOI 10.1002/pmic.201000092
Idioma: | Português
Conteúdo: | The economic and ecological importance of the symbiosis of soybean with Bradyrhizobium japonicum strains is significant in several countries, particularly Brazil; however, up to now, only one complete and a draft genome for this species are available. In this study, we have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) ? used in commercial inoculants for application to soybean crops in Brazil ? grown under in vitro conditions. CPAC 15 belongs to the same serogroup as strain USDA 123, and both are known as the soybean bradyrhizobial strains with highest competitive and saprophytic known so far. To increase the precision of the proteomic map, we compared whole-cell 2-D protein gel-electrophoresis profiles of CPAC 15 and of two related strains. One-hundred and seventy representative spots, selected from the three profiles, were analyzed by MS. In total, 148 spots were successfully identified as cytoplasmic and periplasmic proteins belonging to diverse metabolic pathways, several of them related to the saprophytic and competitive abilities of CPAC 15. We attributed probable functions to 26 hypothetical proteins, including those involved in polyhydroxybutyrate metabolism, ?-lactamase, stress responses and aromatic compound degradation, all with high probability of being related to the saprophytic ability of CPAC 15. In addition, by providing valuable information about expressed proteins in B. japonicum in vitro, our results emphasize the importance of accurate functional annotation of uncharacterized expressed proteins, improving considerably our understanding of the legume-rhizobia symbiosis.
Thesagro: | Bacteria; Bradyrhizobium Japonicum; Microbiologia; Soja.
Thesaurus Nal: | Microsymbionts; Soybeans.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (31200-1) - UPC - AP - PP - 11868 -  - 11868 - DIS