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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
16/08/2023 |
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Data da última atualização: |
09/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BATISTA, D. da C.; VIEIRA, W. A. dos S.; BARBOSA, M. A. G.; CÂMARA, M. P. S. |
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Afiliação: |
DIOGENES DA CRUZ BATISTA, CPATSA; WILLIE ANDERSON DOS SANTOS VIEIRA, UFRPE; MARIA ANGELICA GUIMARAES BARBOSA, CPATSA; MARCOS PAZ SARAIVA CÂMARA, UFRPE. |
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Título: |
First report of Colletotrichum siamense causing grape ripe rot in Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant Disease, v. 107, n. 9, p. 2881, May 2023. |
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DOI: |
https://doi.org/10.1094/PDIS-11-22-2589-PDN |
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Idioma: |
Inglês |
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Conteúdo: |
Vitis vinifera cv. BRS Vitória is a seedless black table grape cultivar with an extremely pleasant flavor and is adapted to cultivation in all Brazilian regions. Between November and December 2021, grape berries presenting typical ripe rot symptoms were found in three vineyards located in Petrolina, Pernambuco Brazil. The first symptoms are small and depressed lesions on ripe berries, presenting tiny black acervuli. As the disease progress, lesions enlarge and affect the whole fruit, and abundant orange masses of conidia can be observed. Finally, berries become completely mummified. Symptoms were observed in the three vineyards visited, and disease incidence was above 90%. Some producers are considering eradicating the plantations due to the losses caused by the disease. Control measures used so far are costly and ineffective. Fungal isolation was performed by transferring conidial masses from 10 diseased fruit to plates containing potato dextrose agar medium. Cultures were incubated at 25 °C under continuous light. Seven days after inoculation, three fungal isolates (LM1543?1545) were obtained and subcultivated in pure culture for species identification and pathogenicity test. Isolates presented white to grey cottony mycelia, and hyaline conidia with cylindrical with rounded ends, which resemble the genus Colletotrichum (Sutton 1980). Partial sequences of APN2-MAT/IGS, CAL, and GAPDH loci were amplified, sequenced, and deposited on GenBank (OP643865?OP643872). Isolates from V. vinifera were placed within the clade including the ex-type and representative isolates of C. siamense. The clade was strongly supported (99.8% bootstrap support) in the maximum likelihood multilocus tree of the three loci combined, which confidently assign the isolates to this species. Inoculation on grape bunches was performed to confirm pathogenicity. Grape bunches were surface sterilized for 30 s in 70% ethanol, 1 min in 1.5% NaOCl, rinsed two times with sterile distilled water, and air-dried. Fungal conidial suspensions (106 conidia mL-1) were sprayed to the point of run-off. The negative control was represented by grape bunches sprayed with sterile distilled water. Grape bunches were kept in a humid chamber for 48 h at 25 °C and a light period of 12 h. The experiment was conducted with four replicates (four inoculated bunches per isolate) and repeated once. Typical ripe rot symptoms were observed on grape berries 7 days after inoculation. No symptoms were observed on the negative control. The fungal isolates recovered from inoculated berries were morphologically identical to the C. siamense isolates originally recovered from symptomatic berries collected in the field, fulfilling Koch?s postulates. Colletotrichum siamense was reported in association with grape leaves in USA (Weir et al. 2012) and causing grape ripe rot in North America (Cosseboom & Hu 2022). Only C. fructicola, C. kahawae, C. karsti, C. limetticola, C. nymphaeae and C. viniferum were reported causing grape ripe rot in Brazil (Echeverrigaray et al. 2020). To our knowledge, this is the first report of C. siamense causing grape ripe rot in Brazil. This finding is important for disease management because C. siamense has a high phytopathogenic potential due to its wide distribution and host range. MenosVitis vinifera cv. BRS Vitória is a seedless black table grape cultivar with an extremely pleasant flavor and is adapted to cultivation in all Brazilian regions. Between November and December 2021, grape berries presenting typical ripe rot symptoms were found in three vineyards located in Petrolina, Pernambuco Brazil. The first symptoms are small and depressed lesions on ripe berries, presenting tiny black acervuli. As the disease progress, lesions enlarge and affect the whole fruit, and abundant orange masses of conidia can be observed. Finally, berries become completely mummified. Symptoms were observed in the three vineyards visited, and disease incidence was above 90%. Some producers are considering eradicating the plantations due to the losses caused by the disease. Control measures used so far are costly and ineffective. Fungal isolation was performed by transferring conidial masses from 10 diseased fruit to plates containing potato dextrose agar medium. Cultures were incubated at 25 °C under continuous light. Seven days after inoculation, three fungal isolates (LM1543?1545) were obtained and subcultivated in pure culture for species identification and pathogenicity test. Isolates presented white to grey cottony mycelia, and hyaline conidia with cylindrical with rounded ends, which resemble the genus Colletotrichum (Sutton 1980). Partial sequences of APN2-MAT/IGS, CAL, and GAPDH loci were amplified, sequenced, and deposited on GenBank (OP643865?OP643872). Isolates from... Mostrar Tudo |
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Palavras-Chave: |
BRS Vitória; Colletotrichum siamense; Podridão madura; Uva de mesa; Uva sem sementes. |
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Thesagro: |
Doença; Fungo; Uva; Vitis Vinifera. |
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Thesaurus Nal: |
Grapes; Seedless varieties. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 04159naa a2200301 a 4500
001 2155944
005 2024-01-09
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1094/PDIS-11-22-2589-PDN$2DOI
100 1 $aBATISTA, D. da C.
245 $aFirst report of Colletotrichum siamense causing grape ripe rot in Brazil.$h[electronic resource]
260 $c2023
520 $aVitis vinifera cv. BRS Vitória is a seedless black table grape cultivar with an extremely pleasant flavor and is adapted to cultivation in all Brazilian regions. Between November and December 2021, grape berries presenting typical ripe rot symptoms were found in three vineyards located in Petrolina, Pernambuco Brazil. The first symptoms are small and depressed lesions on ripe berries, presenting tiny black acervuli. As the disease progress, lesions enlarge and affect the whole fruit, and abundant orange masses of conidia can be observed. Finally, berries become completely mummified. Symptoms were observed in the three vineyards visited, and disease incidence was above 90%. Some producers are considering eradicating the plantations due to the losses caused by the disease. Control measures used so far are costly and ineffective. Fungal isolation was performed by transferring conidial masses from 10 diseased fruit to plates containing potato dextrose agar medium. Cultures were incubated at 25 °C under continuous light. Seven days after inoculation, three fungal isolates (LM1543?1545) were obtained and subcultivated in pure culture for species identification and pathogenicity test. Isolates presented white to grey cottony mycelia, and hyaline conidia with cylindrical with rounded ends, which resemble the genus Colletotrichum (Sutton 1980). Partial sequences of APN2-MAT/IGS, CAL, and GAPDH loci were amplified, sequenced, and deposited on GenBank (OP643865?OP643872). Isolates from V. vinifera were placed within the clade including the ex-type and representative isolates of C. siamense. The clade was strongly supported (99.8% bootstrap support) in the maximum likelihood multilocus tree of the three loci combined, which confidently assign the isolates to this species. Inoculation on grape bunches was performed to confirm pathogenicity. Grape bunches were surface sterilized for 30 s in 70% ethanol, 1 min in 1.5% NaOCl, rinsed two times with sterile distilled water, and air-dried. Fungal conidial suspensions (106 conidia mL-1) were sprayed to the point of run-off. The negative control was represented by grape bunches sprayed with sterile distilled water. Grape bunches were kept in a humid chamber for 48 h at 25 °C and a light period of 12 h. The experiment was conducted with four replicates (four inoculated bunches per isolate) and repeated once. Typical ripe rot symptoms were observed on grape berries 7 days after inoculation. No symptoms were observed on the negative control. The fungal isolates recovered from inoculated berries were morphologically identical to the C. siamense isolates originally recovered from symptomatic berries collected in the field, fulfilling Koch?s postulates. Colletotrichum siamense was reported in association with grape leaves in USA (Weir et al. 2012) and causing grape ripe rot in North America (Cosseboom & Hu 2022). Only C. fructicola, C. kahawae, C. karsti, C. limetticola, C. nymphaeae and C. viniferum were reported causing grape ripe rot in Brazil (Echeverrigaray et al. 2020). To our knowledge, this is the first report of C. siamense causing grape ripe rot in Brazil. This finding is important for disease management because C. siamense has a high phytopathogenic potential due to its wide distribution and host range.
650 $aGrapes
650 $aSeedless varieties
650 $aDoença
650 $aFungo
650 $aUva
650 $aVitis Vinifera
653 $aBRS Vitória
653 $aColletotrichum siamense
653 $aPodridão madura
653 $aUva de mesa
653 $aUva sem sementes
700 1 $aVIEIRA, W. A. dos S.
700 1 $aBARBOSA, M. A. G.
700 1 $aCÂMARA, M. P. S.
773 $tPlant Disease$gv. 107, n. 9, p. 2881, May 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 119 | |
| 5. |  | BATISTA, D. da C.; BARBOSA, M. A. G. Doenças da mangueira. In: SIMPÓSIO INTERNACIONAL DE VITIVINICULTURA, 1.; FEIRA NACIONAL DA AGRICULTURA IRRIGADA - FENAGRI, 2008, Petrolina. Minicursos. Petrolina: Prefeitura Municipal: ValeXport: Embrapa Semi-Árido, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Semiárido. |
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| 6. | | TERAO, D.; BATISTA, D. da C.; RIBEIRO, I. J. A. Doenças da mangueira. In: AMORIM, L.; REZENDE, J. A. M; BERGAMIM FILHO, A.; CAMARGO, L. E. A (Ed.). Manual de fitopatologia: doenças das plantas cultivadas. 5. ed. Piracicaba: Ceres, 2016. v.2 Cap. 54, p. 523-533.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Semiárido. |
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| 7. | | TERAO, D.; BATISTA, D. da C.; RIBEIRO, I. J. A. Doenças da mangueira. In: AMORIM, L.; REZENDE, J. A. M; BERGAMIM FILHO, A.; CAMARGO, L. E. A (Ed.). Manual de fitopatologia: doenças das plantas cultivadas. 5. ed. Piracicaba: Ceres, 2016. v.2 Cap. 54, p. 523-533.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | BATISTA, D. da C.; TERAO, D.; BARBOSA, M. A. G. Impacto da cobertura plástica na intensidade do oídio da videira. Tropical Plant Pathology, Brasília, DF, v. 33, p. S 180, ago. 2008. Suplemento. Ref. EPI-032. Edição dos Resumos do 41. Congresso Brasileiro de Fitopatologia, Belo Horizonte, ago. 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 19. | | BARBOSA, M. A. G.; BATISTA, D. da C.; TERAO, D.; ANJOS, F. G. J. dos. Avaliação de métodos de inoculação para Fusicoccum spp., Lasiodiplodia theobromae e Colletotrichum gloeosporioides em manga. Summa Phytopathologica, v. 35, fev. 2009. Suplemento, ref. 149. Edição dos Resumos do 32 Congresso Paulista de Fitopatologia; 4 Reunião Brasileira sobre Indução de Resistência em Plantas a Patógenos, São Pedro, fev. 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 20. | | ANGELOTTI, F.; GAVA, C. A. T.; BATISTA, D. da C.; SANTOS, T. T. C. Avaliação de sistemas de alerta para o míldio da videira no Vale do São Francisco. Tropical Plant Pathology, Brasília, DF, v. 34, p. S117, ago. 2009. Suplemento. Edição dos Resumos do 42 Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 119 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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