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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
10/05/2011 |
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Data da última atualização: |
04/08/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
STEIBEL, J. P.; BATES, R. O.; ROSA, G. J. M.; TEMPELMAN, R. J.; RILINGTON, V. D.; RAGAVENDRAN, A.; RANEY, NANCY E.; RAMOS, A. M.; CARDOSO, F. F.; EDWARDS, D. B.; ERNST, C. W. |
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Afiliação: |
JUAN PEDRO STEIBEL, Department of Animal Science, Michigan State University; RONALD O. BATES, Department of Animal Science, Michigan State University; GUILHERME J. M. ROSA, Department of Animal Sciences, University of Wisconsin; ROBERT J. TEMPELMAN, Department of Animal Science, Michigan State University; VALENCIA D. RILINGTON, Department of Animal Science, Michigan State University; ASHOK RAGAVENDRAN, Department of Animal Science, Michigan State University; NANCY E. RANEY, Department of Animal Science, Michigan State University; ANTONIO MARCOS RAMOS, Department of Animal Science, Michigan State University; FERNANDO FLORES CARDOSO, CPPSUL; DAVID B. EDWARDS, Department of Animal Science, Michigan State University; ERNST, C. W., Department of Animal Science, Michigan State University. |
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Título: |
Genome-wide linkage analysis of global gene expression in loin muscle tissue identifies candidate genes in pigs. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
PLoS ONE, San Francisco, v. 6, n. 2, e16766, Feb. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Nearly 6,000 QTL have been reported for 588 different traits in pigs, more than in any other livestock species. However, this effort has translated into only a few confirmed causative variants. A powerful strategy for revealing candidate genes involves expression QTL (eQTL) mapping, where the mRNA abundance of a set of transcripts is used as the response variable for a QTL scan. Methodology/Principal Findings: We utilized a whole genome expression microarray and an F2 pig resource population to conduct a global eQTL analysis in loin muscle tissue, and compared results to previously inferred phenotypic QTL (pQTL) from the same experimental cross. We found 62 unique eQTL (FDR ,10%) and identified 3 gene networks enriched with genes subject to genetic control involved in lipid metabolism, DNA replication, and cell cycle regulation. We observed strong evidence of local regulation (40 out of 59 eQTL with known genomic position) and compared these eQTL to pQTL to help identify potential candidate genes. Among the interesting associations, we found aldo-keto reductase 7A2 (AKR7A2) and thioredoxin domain containing 12 (TXNDC12) eQTL that are part of a network associated with lipid metabolism and in turn overlap with pQTL regions for marbling, % intramuscular fat (% fat) and loin muscle area on Sus scrofa (SSC) chromosome 6. Additionally, we report 13 genomic regions with overlapping eQTL and pQTL involving 14 local eQTL. Conclusions/Significance: Results of this analysis provide novel candidate genes for important complex pig phenotypes. MenosBackground: Nearly 6,000 QTL have been reported for 588 different traits in pigs, more than in any other livestock species. However, this effort has translated into only a few confirmed causative variants. A powerful strategy for revealing candidate genes involves expression QTL (eQTL) mapping, where the mRNA abundance of a set of transcripts is used as the response variable for a QTL scan. Methodology/Principal Findings: We utilized a whole genome expression microarray and an F2 pig resource population to conduct a global eQTL analysis in loin muscle tissue, and compared results to previously inferred phenotypic QTL (pQTL) from the same experimental cross. We found 62 unique eQTL (FDR ,10%) and identified 3 gene networks enriched with genes subject to genetic control involved in lipid metabolism, DNA replication, and cell cycle regulation. We observed strong evidence of local regulation (40 out of 59 eQTL with known genomic position) and compared these eQTL to pQTL to help identify potential candidate genes. Among the interesting associations, we found aldo-keto reductase 7A2 (AKR7A2) and thioredoxin domain containing 12 (TXNDC12) eQTL that are part of a network associated with lipid metabolism and in turn overlap with pQTL regions for marbling, % intramuscular fat (% fat) and loin muscle area on Sus scrofa (SSC) chromosome 6. Additionally, we report 13 genomic regions with overlapping eQTL and pQTL involving 14 local eQTL. Conclusions/Significance: Results of this analysis... Mostrar Tudo |
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Thesagro: |
Genética; Suíno. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/33973/1/METAS-2011-FFC-Steibel-et-al-2011-PLoS-ONE-62-e16766.pdf
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Marc: |
LEADER 02341naa a2200265 a 4500
001 1888216
005 2011-08-04
008 2011 bl uuuu u00u1 u #d
100 1 $aSTEIBEL, J. P.
245 $aGenome-wide linkage analysis of global gene expression in loin muscle tissue identifies candidate genes in pigs.$h[electronic resource]
260 $c2011
520 $aBackground: Nearly 6,000 QTL have been reported for 588 different traits in pigs, more than in any other livestock species. However, this effort has translated into only a few confirmed causative variants. A powerful strategy for revealing candidate genes involves expression QTL (eQTL) mapping, where the mRNA abundance of a set of transcripts is used as the response variable for a QTL scan. Methodology/Principal Findings: We utilized a whole genome expression microarray and an F2 pig resource population to conduct a global eQTL analysis in loin muscle tissue, and compared results to previously inferred phenotypic QTL (pQTL) from the same experimental cross. We found 62 unique eQTL (FDR ,10%) and identified 3 gene networks enriched with genes subject to genetic control involved in lipid metabolism, DNA replication, and cell cycle regulation. We observed strong evidence of local regulation (40 out of 59 eQTL with known genomic position) and compared these eQTL to pQTL to help identify potential candidate genes. Among the interesting associations, we found aldo-keto reductase 7A2 (AKR7A2) and thioredoxin domain containing 12 (TXNDC12) eQTL that are part of a network associated with lipid metabolism and in turn overlap with pQTL regions for marbling, % intramuscular fat (% fat) and loin muscle area on Sus scrofa (SSC) chromosome 6. Additionally, we report 13 genomic regions with overlapping eQTL and pQTL involving 14 local eQTL. Conclusions/Significance: Results of this analysis provide novel candidate genes for important complex pig phenotypes.
650 $aGenética
650 $aSuíno
700 1 $aBATES, R. O.
700 1 $aROSA, G. J. M.
700 1 $aTEMPELMAN, R. J.
700 1 $aRILINGTON, V. D.
700 1 $aRAGAVENDRAN, A.
700 1 $aRANEY, NANCY E.
700 1 $aRAMOS, A. M.
700 1 $aCARDOSO, F. F.
700 1 $aEDWARDS, D. B.
700 1 $aERNST, C. W.
773 $tPLoS ONE, San Francisco$gv. 6, n. 2, e16766, Feb. 2011.
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Registro original: |
Embrapa Pecuária Sul (CPPSUL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
04/03/2010 |
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Data da última atualização: |
10/08/2017 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
COELHO, M. R. R.; CARNEIRO, N. P.; MARRIEL, I. E.; SELDIN, L. |
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Afiliação: |
MARCIA REED RODRIGUES COELHO, UFRJ; NEWTON PORTILHO CARNEIRO, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; LUCY SELDIN, UFRJ. |
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Título: |
Método molecular para estudos ecológicos de bactérias diazotróficas do gênero Paenibacillus em amostras ambientais. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Sete Lagoas: Embrapa Milho e Sorgo, 2009. |
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Páginas: |
26 p. |
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Série: |
(Embrapa Milho e Sorgo. Boletim de Pesquisa e Desenvolvimento, 10). |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi desenvolver e testar um protocolo de PCR específico para a amplificação de espécies de bactérias fixadoras de nitrogênio do gênero Paenibacillus, utilizando-se a estratégia de clonagem e sequenciamento do gene nifH. . |
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Thesagro: |
Fixação de Nitrogênio; Genética; Microbiologia do Solo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPMS-2010/22417/1/Bol-10.pdf
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Marc: |
LEADER 00945nam a2200205 a 4500
001 1659824
005 2017-08-10
008 2009 bl uuuu u0uu1 u #d
100 1 $aCOELHO, M. R. R.
245 $aMétodo molecular para estudos ecológicos de bactérias diazotróficas do gênero Paenibacillus em amostras ambientais.$h[electronic resource]
260 $aSete Lagoas: Embrapa Milho e Sorgo$c2009
300 $a26 p.
490 $a(Embrapa Milho e Sorgo. Boletim de Pesquisa e Desenvolvimento, 10).
520 $aO objetivo deste trabalho foi desenvolver e testar um protocolo de PCR específico para a amplificação de espécies de bactérias fixadoras de nitrogênio do gênero Paenibacillus, utilizando-se a estratégia de clonagem e sequenciamento do gene nifH. .
650 $aFixação de Nitrogênio
650 $aGenética
650 $aMicrobiologia do Solo
700 1 $aCARNEIRO, N. P.
700 1 $aMARRIEL, I. E.
700 1 $aSELDIN, L.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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