Data/hora: 01/06/2024 14:10:46

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 23/03/2009
Data da última atualização: | 05/02/2024
Autoria: | SANTOS, O. C. S.; BARROS, E. M.; BRITO, M. A. V. P.; BASTOS, M. C. F.; SANTOS, K. R. N.; GIAMBIAGI-DE-MARVAL, M.
Afiliação: | Olinda Cabral da Silva Santos, UFRJ; Elaine Menezes Barros, UFRJ; Maria Aparecida Vasconcelos Paiva Brito, Embrapa Gado de Leite; Maria do Carmo de Freire Bastos, UFRJ; Kátia Regina Netto dos Santos, UFRJ; Marcia Giambiagi-deMarval, UFRJ.
Título: | Identification of coagulase-negative staphylococci from bovine mastitis using RFLP-PCR of the groEL gene.
Ano de publicação: | 2008
Fonte/Imprenta: | Veterinary Microbiology, n. 130, n. 1/2, p. 134-140, 2008.
DOI: | https://doi.org/10.1016/j.vetmic.2007.12.009
Idioma: | Inglês
Conteúdo: | Coagulase-negative staphylococci (CNS) have become the predominant pathogens causing bovine mastitis in many countries. CNS infections are associated with damage to milk secretory tissue of the mammary gland by increased connective tissue stroma, moderate increases of somatic cells count in milk and significant production decreases. These consequences impose serious economic losses for the farmers and the dairy industry. Routine veterinary laboratories do not usually identify CNS at the species level. Thereby, the aims of this study were to identify the most common staphylococcal pathogens involved in bovine mastitis using PCR-restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and to compare our results with the identification carried out by the conventional method. A total of 54 isolates of Staphylococcus, involved in bovine mastitis, were analyzed by this method. The size and number of the fragments obtained by either AluI or HindIII/PvuII digestions made possible to form clear patterns differentiating, among the isolates, 11 of the most common species of animal staphylococcal pathogens. Most of the isolates clustered together with the reference strain of Staphylococcus chromogenes (28) and the type strain of Staphylococcus epidermidis (8). Besides, some isolates clustered together with the type strain of Staphylococcus aureus (5). All patterns were confirmed by the conventional biochemical method, showing concordant results. Thus, the PCR-RFLP of the groEL gene constitutes a reliable and reproducible molecular method for identification of CNS species responsible for bovine mastitis.
Palavras-Chave: | Coagulase-negative staphylococci; groEL gene.
Thesaurus Nal: | bovine mastitis; Staphylococcus.
Categoria do assunto: | --
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (16328-1) - UPC - AP - DD -  -  -  - DIS