Data/hora: 07/06/2024 14:03:58

Biblioteca(s): 	Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Suínos e Aves.	
Data corrente: 	24/08/2023	
Data da última atualização: 	04/09/2023	
Autoria: 	FONSECA, F. M. da; HAACH, V.; BELLAVER, F. V.; BOMBASSARO, G.; GAVA, D.; SILVA, L. P. da; BARON, L. F.; SIMONELLY, M.; CARVALHO, W. A.; SCHAEFER, R.; BASTOS, A. P. A.	
Afiliação: 	FRANCISCO NOE DA FONSECA, GEM; VANESSA HAACH, Universidade Federal do Rio Grande do Sul; FRANCIANA VOLPATO BELLAVER, Instituto Federal Catarinense; GABRIELLY BOMBASSARO, Instituto Federal Catarinense; DANIELLE GAVA, CNPSA; LUCIANO PAULINO DA SILVA, Cenargen; LANA FLAVIA BARON, Universidade Federal do Rio Grande do Sul; MAYARA SIMONELLY, Universidade de Brasília; WANESSA ARAUJO CARVALHO, CNPGL; REJANE SCHAEFER, CNPSA; ANA PAULA ALMEIDA BASTOS, CNPSA.	
Título: 	Immunological profile of mice immunized with a polyvalent virosome-based influenza vaccine.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Virology Journal, v. 20, 2023. Article number 187.	
DOI: 	https://doi.org/10.1186/s12985-023-02158-0	
Idioma: 	Inglês	
Notas: 	Na publicação: Francisco Noé Fonseca; Ana Paula Bastos.	
Conteúdo: 	Abstract
Background: Influenza A virus (IAV) causes respiratory disease in pigs and is a major concern for public health.
Vaccination of pigs is the most successful measure to mitigate the impact of the disease in the herds. Influenza-based
virosome is an effective immunomodulating carrier that replicates the natural antigen presentation pathway and has
tolerability profile due to their purity and biocompatibility.
Methods: This study aimed to develop a polyvalent virosome influenza vaccine containing the hemagglutinin and
neuraminidase proteins derived from the swine IAVs (swIAVs) H1N1, H1N2 and H3N2 subtypes, and to investigate
its effectiveness in mice as a potential vaccine for swine. Mice were immunized with two vaccine doses (1 and 15
days), intramuscularly and intranasally. At 21 days and eight months later after the second vaccine dose, mice were
euthanized. The humoral and cellular immune responses in mice vaccinated intranasally or intramuscularly with a
polyvalent influenza virosomal vaccine were investigated.
Results: Only intramuscular vaccination induced high hemagglutination inhibition (HI) titers. Seroconversion
and seroprotection (>4-fold rise in HI antibody titers, reaching a titer of ?1:40) were achieved in 80% of mice
(intramuscularly vaccinated group) at 21 days after booster immunization. Virus-neutralizing antibody titers against
IAV were detected at 8 months after vaccination, indicating long-lasting immunity. Overall, mice immunized with the
virosome displayed greater ability for B, effector-T and memory-T cells from the spleen to respond to H1N1, H1N2 and
H3N2 antigens.
Conclusions: All findings showed an efficient immune response against IAVs in mice vaccinated with a polyvalent
virosome-based influenza vaccine.	
Palavras-Chave: 	Nanovaccine; Nanovacina; Nanovacinação; Seroprotection; Soro imunológico; Soro proteção; Soroproteção; Vaccine.	
Thesagro: 	Influenza Aviaria; Vacina; Vacinação; Vírus.	
Thesaurus Nal: 	Influenza A virus; Vaccination.	
Categoria do assunto: 	--
X Pesquisa, Tecnologia e Engenharia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156329/1/Immunological-profile-of-mice-immunized-with-a-polyvalent-virosome.pdf	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CENARGEN - (39212-1) - UPC - AP - DD -  -  -  - DIS
CNPSA - (22585-1) - UPC - AP - DD -  -  -  - DIS
CNPGL - (26089-1) - UPC - AP - DD -  -  -  - DIS