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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
30/03/2009 |
Data da última atualização: |
30/06/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
KITAJIMA, E. W.; CALEGARIO, R. F.; NOVELLI, V. M.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; FRANCISCHINI, F.; FREITAS-ASTUA, J. |
Afiliação: |
Elliot Watanabe Kitajima, ESALQ; R. F. Calegario, ESALQ; Valdenice Moreira Novelli, APTA; Eliane Cristina Locali-Fabris, APTA; Marinês Bastianel, APTA; F. Francischini, Alellyx Genomics; Juliana Freitas-Ástua, CNPMF. |
Título: |
In situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. |
Descrição Física: |
1 CD ROM |
Idioma: |
Inglês |
Conteúdo: |
Among the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurred between membranes of adjacent cells near the midgut and prosomal gland. Anti-NC antibodies specifically labeled these particles both in the plant and in the mite thus confirming their viral nature. Viroplasmas, present in infected plant cells, are also immunolabeled by anti-NC antibody, but they were not found in the mite tissues. A possible mechanism to explain the viral presence between cells, similar to diapedesis of the leucocytes in blood vessels, is being suggested. These cytological evidences in the mite reinforce the concept that the CiLV-C/Brevipalpus relation is of circulative rather than replicative type. This information is relevant for epidemiology and control strategies. MenosAmong the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurr... Mostrar Tudo |
Thesagro: |
Ácaro; Vírus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03084nam a2200217 a 4500 001 1655634 005 2023-06-30 008 2008 bl uuuu u00u1 u #d 100 1 $aKITAJIMA, E. W. 245 $aIn situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type.$h[electronic resource] 260 $aIn: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.]$c2008 300 $c1 CD ROM 520 $aAmong the Brevipalpus (Acari: Tenuipalpidae)-transmitted plant viruses (BTV), citrus leprosis, cytoplasmic type (CiLV-C) is, by far, the most important. It causes localized lesions on the leaves, stems, and fruits and untreated plants may die within few years. Progresses have been made on the nature of CiLV-C. Infection by the virus induces a characteristic eletron dense and vacuolated inclusion (viroplasm) in the cytoplasm and the occurence of short, bacilliform virions of CiLV-C within endoplasmic reticulum. Its genome was completely sequenced being a bipartite (6 and 9 kb) positive sense ssRNA with a poly-A tail, different from other known viruses and a new genus Cilevirus was proposed for CiLV-C. Primers were designed for specific detection of CiLV-C and some of the viral proteins were expressed in vitro, including nucleocapsid (NC) protein and a specific antibody is available. The precise relationship between CiLV-C and its vector B. phoenicis has remained unclear. Recent works permitted to detect CiLV-C in the mite by RT - PCR and RT -qPCR assays suggest that the virus does not replicate in the mite. Transmission assays revealing that larvae and nymphs are also able to transmit CiLV-C are considered additional evidences for the absence of replication of the virus in the mite. Transmission electron microscopy of sections of viruliferous B. phoenicis allowed the visualization of virus-like particles as seen in the plant cells, in the mite bodies. they consistently occurred between membranes of adjacent cells near the midgut and prosomal gland. Anti-NC antibodies specifically labeled these particles both in the plant and in the mite thus confirming their viral nature. Viroplasmas, present in infected plant cells, are also immunolabeled by anti-NC antibody, but they were not found in the mite tissues. A possible mechanism to explain the viral presence between cells, similar to diapedesis of the leucocytes in blood vessels, is being suggested. These cytological evidences in the mite reinforce the concept that the CiLV-C/Brevipalpus relation is of circulative rather than replicative type. This information is relevant for epidemiology and control strategies. 650 $aÁcaro 650 $aVírus 700 1 $aCALEGARIO, R. F. 700 1 $aNOVELLI, V. M. 700 1 $aLOCALI-FABRIS, E. C. 700 1 $aBASTIANEL, M. 700 1 $aFRANCISCHINI, F. 700 1 $aFREITAS-ASTUA, J.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
06/12/2016 |
Data da última atualização: |
06/12/2016 |
Autoria: |
SOUSA, I. K. F.; SOUSA, R. S.; AZEVEDO, A. C. P.; CORRÊA, I. F. C. B.; OLIVEIRA, J. M. de; MATOS, S. P.; MORI, C. S.; ORTOLANI, E. L. |
Afiliação: |
ISADORA K. F. SOUSA, UFOPA; REJANE S. SOUSA, FMVZ/USP; ADNA C. P. AZEVEDO, Faculdades Integradas do Tapajós; ILVA F. C. B. CORRÊA, Faculdades Integradas do Tapajós; JAIRO MOURA DE OLIVEIRA, Faculdades Integradas do Tapajós; SIDCLEY P. MATOS, Jardim Zoológico, Faculdades Integradas do Tapajós; CLARA S. MORI, FMVZ/USP; ENRICO L. ORTOLANI, FMVZ/USP. |
Título: |
Variáveis hematológicas e bioquímicas de peixe-boi da Amazônia (Trichechus inungui) jovens. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 39, n. 9, p. 869-873, set. 2016. |
Idioma: |
Português |
Conteúdo: |
Peixes-boi da Amazônia (Trichechus inungui) jovens são frequentemente encontrados e resgatados de rios, no entanto para avaliar e monitorar o estado de saúde desses animais é necessário conhecer os valores de referência das variáveis hematológicas e bioquímicas nessa faixa etária. O trabalho objetivou determinar os valores hematológicos e bioquímicos de peixes-boi da amazônia jovens e saudáveis, mantidos em cativeiros. Foram coletadas 20 amostras sanguíneas de peixes-boi para a realização do hemograma e bioquímica. Os valores médios e desvio padrão das variáveis hematológicas foram: volume globular, 37±4%; eritrócitos, 4,4±0,35x106/μL; hemoglo-bina, 12,5±1,6g/dL; volume corpuscular médio (VCM), 85,33±4,28fL; hemoglobina corpuscular media (HCM), 28,29±1,39pg; e concentração de hemoglobina corpuscular media (CHCM), 33,13±0,20%, leucócitos totais, 9.080±1.868x103/μL; segmentados, 5.078±1.044,24x103/ μL; linfócitos 3.556±1,063x103/μL; eosinófilos, 288± 215,51x103/μL; e monócitos, 165± 114,11x103/μL. A média e o intervalo (mínimo e máximo) das variáveis bioquímicas foram: creatinina quinase (CK), 112,53 (50,10- 295,80) U/L; aspartato aminotransferase (AST), 6,02 (2,80-10,20) U/L; gama glutamiltransferase (GGT), 25,24 (10,80-45,30) U/L; fosfatase alcalina (FA), 98,73 (14,66-198,30) U/L; proteína total, 6,12 (5,38-7,10) g/dL; albumina, 4,23 (3,50-5,00) g/dL; colesterol, 310,13 (144-518) mg/dL; triglicerídeos, 127,98 (57,50-213,50) mg/dL; glicose, 43,63 (24,50-73,80) mg/dL; ureia, 30,21 (14,30-54,25); creatinina, 1,34 (0,93-1,76) mg/dL; cálcio, 10,08 (9,25-11,10) mg/dL; e fósforo inorgânico; 6,50 (4,10-8,80) mg/dL. No presente trabalho foi possível determinar valores hematológicos e bioquímicos de peixes-boi da Amazônia (Trichechus inungui) jovens, mantidos em cativeiro, os quais podem ser utilizados como referência para animais em cativeiro e de vida livre, mantidos em condições ambientais, e de manejo, semelhantes. MenosPeixes-boi da Amazônia (Trichechus inungui) jovens são frequentemente encontrados e resgatados de rios, no entanto para avaliar e monitorar o estado de saúde desses animais é necessário conhecer os valores de referência das variáveis hematológicas e bioquímicas nessa faixa etária. O trabalho objetivou determinar os valores hematológicos e bioquímicos de peixes-boi da amazônia jovens e saudáveis, mantidos em cativeiros. Foram coletadas 20 amostras sanguíneas de peixes-boi para a realização do hemograma e bioquímica. Os valores médios e desvio padrão das variáveis hematológicas foram: volume globular, 37±4%; eritrócitos, 4,4±0,35x106/μL; hemoglo-bina, 12,5±1,6g/dL; volume corpuscular médio (VCM), 85,33±4,28fL; hemoglobina corpuscular media (HCM), 28,29±1,39pg; e concentração de hemoglobina corpuscular media (CHCM), 33,13±0,20%, leucócitos totais, 9.080±1.868x103/μL; segmentados, 5.078±1.044,24x103/ μL; linfócitos 3.556±1,063x103/μL; eosinófilos, 288± 215,51x103/μL; e monócitos, 165± 114,11x103/μL. A média e o intervalo (mínimo e máximo) das variáveis bioquímicas foram: creatinina quinase (CK), 112,53 (50,10- 295,80) U/L; aspartato aminotransferase (AST), 6,02 (2,80-10,20) U/L; gama glutamiltransferase (GGT), 25,24 (10,80-45,30) U/L; fosfatase alcalina (FA), 98,73 (14,66-198,30) U/L; proteína total, 6,12 (5,38-7,10) g/dL; albumina, 4,23 (3,50-5,00) g/dL; colesterol, 310,13 (144-518) mg/dL; triglicerídeos, 127,98 (57,50-213,50) mg/dL; glicose, 43,63... Mostrar Tudo |
Palavras-Chave: |
Aspartato aminotransferase; Peixe-boi da Amazônia; Trichechus inungui; Wine blood cells. |
Thesagro: |
Bioquímica; Creatinina; Globulo branco; Globulo vermelho; Hematologia. |
Thesaurus NAL: |
Aspartate transaminase; Biochemistry; Creatinine; Erythrocytes; Hematology. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/151332/1/Variaveis-hematologicas.pdf
|
Marc: |
LEADER 03107naa a2200373 a 4500 001 2058064 005 2016-12-06 008 2016 bl uuuu u00u1 u #d 100 1 $aSOUSA, I. K. F. 245 $aVariáveis hematológicas e bioquímicas de peixe-boi da Amazônia (Trichechus inungui) jovens. 260 $c2016 520 $aPeixes-boi da Amazônia (Trichechus inungui) jovens são frequentemente encontrados e resgatados de rios, no entanto para avaliar e monitorar o estado de saúde desses animais é necessário conhecer os valores de referência das variáveis hematológicas e bioquímicas nessa faixa etária. O trabalho objetivou determinar os valores hematológicos e bioquímicos de peixes-boi da amazônia jovens e saudáveis, mantidos em cativeiros. Foram coletadas 20 amostras sanguíneas de peixes-boi para a realização do hemograma e bioquímica. Os valores médios e desvio padrão das variáveis hematológicas foram: volume globular, 37±4%; eritrócitos, 4,4±0,35x106/μL; hemoglo-bina, 12,5±1,6g/dL; volume corpuscular médio (VCM), 85,33±4,28fL; hemoglobina corpuscular media (HCM), 28,29±1,39pg; e concentração de hemoglobina corpuscular media (CHCM), 33,13±0,20%, leucócitos totais, 9.080±1.868x103/μL; segmentados, 5.078±1.044,24x103/ μL; linfócitos 3.556±1,063x103/μL; eosinófilos, 288± 215,51x103/μL; e monócitos, 165± 114,11x103/μL. A média e o intervalo (mínimo e máximo) das variáveis bioquímicas foram: creatinina quinase (CK), 112,53 (50,10- 295,80) U/L; aspartato aminotransferase (AST), 6,02 (2,80-10,20) U/L; gama glutamiltransferase (GGT), 25,24 (10,80-45,30) U/L; fosfatase alcalina (FA), 98,73 (14,66-198,30) U/L; proteína total, 6,12 (5,38-7,10) g/dL; albumina, 4,23 (3,50-5,00) g/dL; colesterol, 310,13 (144-518) mg/dL; triglicerídeos, 127,98 (57,50-213,50) mg/dL; glicose, 43,63 (24,50-73,80) mg/dL; ureia, 30,21 (14,30-54,25); creatinina, 1,34 (0,93-1,76) mg/dL; cálcio, 10,08 (9,25-11,10) mg/dL; e fósforo inorgânico; 6,50 (4,10-8,80) mg/dL. No presente trabalho foi possível determinar valores hematológicos e bioquímicos de peixes-boi da Amazônia (Trichechus inungui) jovens, mantidos em cativeiro, os quais podem ser utilizados como referência para animais em cativeiro e de vida livre, mantidos em condições ambientais, e de manejo, semelhantes. 650 $aAspartate transaminase 650 $aBiochemistry 650 $aCreatinine 650 $aErythrocytes 650 $aHematology 650 $aBioquímica 650 $aCreatinina 650 $aGlobulo branco 650 $aGlobulo vermelho 650 $aHematologia 653 $aAspartato aminotransferase 653 $aPeixe-boi da Amazônia 653 $aTrichechus inungui 653 $aWine blood cells 700 1 $aSOUSA, R. S. 700 1 $aAZEVEDO, A. C. P. 700 1 $aCORRÊA, I. F. C. B. 700 1 $aOLIVEIRA, J. M. de 700 1 $aMATOS, S. P. 700 1 $aMORI, C. S. 700 1 $aORTOLANI, E. L. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 39, n. 9, p. 869-873, set. 2016.
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