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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
27/03/2009 |
Data da última atualização: |
30/06/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
NICOLINI, F.; BASTIANEL, M.; SCHONS, J.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; PEREIRA, J. A.; KUBO, K. S.; NOVELLI, V. M.; MACHADO, M. A.; FREITAS-ASTÚA, J. |
Afiliação: |
F. Nicolini, IAC; M. Bastianel, IAC; J. Schons, UPF; Elliot Watanabe Kitajima, ESALQ; Eliane Cristina Locali-Fabris, IAC; J. A. Pereira, IAC; Karine S. Kubo, IAC; V. M. Novelli, IAC; Marcos Antonio Machado, IAC; Juliana Freitas-Ástua, CNPMF. |
Título: |
Comparação entre os títulos de vírus transmitidos por ácaros Brevipalpus por RT-qPCR. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008. |
Idioma: |
Português |
Conteúdo: |
Comparison among the titers of Brevipalpus transmitted viruses by RT-qPCR. Os vírus transmitidos por Brevipalpus (VTBs) têm em comum, além do vetor, os sintomas nas plantas hospedeiras e a morfologia das partículas. Eles podem ser divididos em tipo nuclear (N) e citoplasmático (C) em função do local de acúmulo nas células. O objetivo do trabalho foi comparar os títulos de alguns VTBs in planta e no ácaro através de RT-qPCR a fim de obter subsídios para classificá-los quanto ao tipo de interação vírus-vetor. Para aquisição dos vírus, os ácaros permaneceram em folhas sintomáticas para Clerodendrum chlorotic spot virus (ClCSV), Coffee ringspot virus (CoRSV) e Citrus leprosis virus (CiLV-C), VTBs-N e VTB-C, respectivamente, por 3 dias. Para análise, três amostras de 10 ácaros, e uma de cada folha sintomática, foram coletadas para quantificação utilizando primers específicos para cada vírus. Os resultados indicaram um título consistentemente maior de CiLV-C in planta do que no vetor. Já para os VTBs-N, a diferença entre os títulos na planta e no vetor foi menos evidente. Esses resultados corroboram dados de microscopia eletrônica que indicam que os VTBs-N replicam no interior do vetor, enquanto os VTBs-C apenas circulam. |
Thesagro: |
Ácaro; Doença de Planta. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01994nam a2200241 a 4500 001 1655622 005 2023-06-30 008 2008 bl uuuu u00u1 u #d 100 1 $aNICOLINI, F. 245 $aComparação entre os títulos de vírus transmitidos por ácaros Brevipalpus por RT-qPCR.$h[electronic resource] 260 $aTropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.$c2008 520 $aComparison among the titers of Brevipalpus transmitted viruses by RT-qPCR. Os vírus transmitidos por Brevipalpus (VTBs) têm em comum, além do vetor, os sintomas nas plantas hospedeiras e a morfologia das partículas. Eles podem ser divididos em tipo nuclear (N) e citoplasmático (C) em função do local de acúmulo nas células. O objetivo do trabalho foi comparar os títulos de alguns VTBs in planta e no ácaro através de RT-qPCR a fim de obter subsídios para classificá-los quanto ao tipo de interação vírus-vetor. Para aquisição dos vírus, os ácaros permaneceram em folhas sintomáticas para Clerodendrum chlorotic spot virus (ClCSV), Coffee ringspot virus (CoRSV) e Citrus leprosis virus (CiLV-C), VTBs-N e VTB-C, respectivamente, por 3 dias. Para análise, três amostras de 10 ácaros, e uma de cada folha sintomática, foram coletadas para quantificação utilizando primers específicos para cada vírus. Os resultados indicaram um título consistentemente maior de CiLV-C in planta do que no vetor. Já para os VTBs-N, a diferença entre os títulos na planta e no vetor foi menos evidente. Esses resultados corroboram dados de microscopia eletrônica que indicam que os VTBs-N replicam no interior do vetor, enquanto os VTBs-C apenas circulam. 650 $aÁcaro 650 $aDoença de Planta 700 1 $aBASTIANEL, M. 700 1 $aSCHONS, J. 700 1 $aKITAJIMA, E. W. 700 1 $aLOCALI-FABRIS, E. C. 700 1 $aPEREIRA, J. A. 700 1 $aKUBO, K. S. 700 1 $aNOVELLI, V. M. 700 1 $aMACHADO, M. A. 700 1 $aFREITAS-ASTÚA, J.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
28/05/2013 |
Data da última atualização: |
02/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
DOMINGUES, L. F.; GIGLIOTI, R.; FEITOSA, K. A.; FANTATTO, R. R.; RABELO, M. D.; OLIVEIRA, M. C. de S.; OLIVEIRA, G. P. de; BECHARA, G. H.; CHAGAS, A. C. de S. |
Afiliação: |
LUCIANA FERREIRA DOMINGUES, Universidade Estadual Paulista; RODRIGO GIGLIOTI, Universidade Estadual Paulista; KARINA ALVES FEITOSA, Centro Universitário Central Paulista; RAFAELA REGINA FANTATTO, Centro Universitário Central Paulista; MARCIO DIAS RABELO, CPPSE; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; GILSON PEREIRA DE OLIVEIRA, CPPSE; GERVASIO HENRIQUE BECHARA, Universidade Estadual Paulista; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
In vitro activity of pineapple extracts (Ananas comosus, Bromeliaceae) on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Experimental Parasitology, v. 134, n .3, p. 400-404, july 2013. |
DOI: |
10.1016/j.exppara.2013.03.021 |
Idioma: |
Inglês |
Conteúdo: |
Measures to control the cattle tick, Rhipicephalus (Boophilus) microplus, based only on chemical products are becoming unsustainable, mainly because of the development of resistance. The objective of this study was to test the effect of the aqueous extract of pineapple skin (AEPS) and bromelain extracted from the stem (Sigma?Aldrich®, B4882) on engorged females and larvae of R. (B.) microplus in vitro. These substances were diluted in water and evaluated at eight concentrations. Engorged females were collected and distributed in groups of 10, with three repetitions for each treatment. After immersion in the solutions, the females were placed in an incubator for observation of survival, oviposition and larval hatching. The larval packet method was used, also with three repetitions with about 100 larvae each. The packets were incubated and the readings were performed after 24 h. The estimated reproduction and efficacy of the solutions were calculated. The LC50 and LC90 were estimated using the Probit procedure of the SAS program. The eight concentrations were compared within each treatment by the Tukey test. For the experiment with engorged females, the most effective concentrations were 125, 250 and 500 mg/mL: 33%, 48% and 59% for the AEPS and 27%, 51% and 55% for the bromelain. The LC50 and LC90 values were, respectively, 276 and 8691 mg/mL for AEPS and 373 and 5172 mg/mL for bromelain. None of the dilutions tested was effective against the larvae of R. (B.) microplus. This is the first report of the action of pineapple extracts or their constituents on cattle ticks. The results demonstrate that further studies regarding composition of tick cuticle, with evaluation of other solvents and formulations, should be conducted seeking to enhance the effect of pineapple extracts and compounds against this ectoparasite. MenosMeasures to control the cattle tick, Rhipicephalus (Boophilus) microplus, based only on chemical products are becoming unsustainable, mainly because of the development of resistance. The objective of this study was to test the effect of the aqueous extract of pineapple skin (AEPS) and bromelain extracted from the stem (Sigma?Aldrich®, B4882) on engorged females and larvae of R. (B.) microplus in vitro. These substances were diluted in water and evaluated at eight concentrations. Engorged females were collected and distributed in groups of 10, with three repetitions for each treatment. After immersion in the solutions, the females were placed in an incubator for observation of survival, oviposition and larval hatching. The larval packet method was used, also with three repetitions with about 100 larvae each. The packets were incubated and the readings were performed after 24 h. The estimated reproduction and efficacy of the solutions were calculated. The LC50 and LC90 were estimated using the Probit procedure of the SAS program. The eight concentrations were compared within each treatment by the Tukey test. For the experiment with engorged females, the most effective concentrations were 125, 250 and 500 mg/mL: 33%, 48% and 59% for the AEPS and 27%, 51% and 55% for the bromelain. The LC50 and LC90 values were, respectively, 276 and 8691 mg/mL for AEPS and 373 and 5172 mg/mL for bromelain. None of the dilutions tested was effective against the larvae of R. (B.) microplus. This ... Mostrar Tudo |
Palavras-Chave: |
Botanical acaricides; Cattle tick; Industrial residue; Phytoterapy. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02725naa a2200277 a 4500 001 1959005 005 2023-05-02 008 2013 bl uuuu u00u1 u #d 024 7 $a10.1016/j.exppara.2013.03.021$2DOI 100 1 $aDOMINGUES, L. F. 245 $aIn vitro activity of pineapple extracts (Ananas comosus, Bromeliaceae) on Rhipicephalus (Boophilus) microplus (Acari$bIxodidae).$h[electronic resource] 260 $c2013 520 $aMeasures to control the cattle tick, Rhipicephalus (Boophilus) microplus, based only on chemical products are becoming unsustainable, mainly because of the development of resistance. The objective of this study was to test the effect of the aqueous extract of pineapple skin (AEPS) and bromelain extracted from the stem (Sigma?Aldrich®, B4882) on engorged females and larvae of R. (B.) microplus in vitro. These substances were diluted in water and evaluated at eight concentrations. Engorged females were collected and distributed in groups of 10, with three repetitions for each treatment. After immersion in the solutions, the females were placed in an incubator for observation of survival, oviposition and larval hatching. The larval packet method was used, also with three repetitions with about 100 larvae each. The packets were incubated and the readings were performed after 24 h. The estimated reproduction and efficacy of the solutions were calculated. The LC50 and LC90 were estimated using the Probit procedure of the SAS program. The eight concentrations were compared within each treatment by the Tukey test. For the experiment with engorged females, the most effective concentrations were 125, 250 and 500 mg/mL: 33%, 48% and 59% for the AEPS and 27%, 51% and 55% for the bromelain. The LC50 and LC90 values were, respectively, 276 and 8691 mg/mL for AEPS and 373 and 5172 mg/mL for bromelain. None of the dilutions tested was effective against the larvae of R. (B.) microplus. This is the first report of the action of pineapple extracts or their constituents on cattle ticks. The results demonstrate that further studies regarding composition of tick cuticle, with evaluation of other solvents and formulations, should be conducted seeking to enhance the effect of pineapple extracts and compounds against this ectoparasite. 653 $aBotanical acaricides 653 $aCattle tick 653 $aIndustrial residue 653 $aPhytoterapy 700 1 $aGIGLIOTI, R. 700 1 $aFEITOSA, K. A. 700 1 $aFANTATTO, R. R. 700 1 $aRABELO, M. D. 700 1 $aOLIVEIRA, M. C. de S. 700 1 $aOLIVEIRA, G. P. de 700 1 $aBECHARA, G. H. 700 1 $aCHAGAS, A. C. de S. 773 $tExperimental Parasitology$gv. 134, n .3, p. 400-404, july 2013.
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