Data/hora: 08/06/2024 00:48:31

Biblioteca(s): | Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido.
Data corrente: | 20/09/2022
Data da última atualização: | 13/01/2023
Autoria: | FRAGOSO, R. da R.; ARRAES, F. B. M.; LOURENCO, I. T.; MIRANDA, V. J.; BASSO, M. F.; FERREIRA, A. V. J.; VIANA, A. A. B.; LINS, C. B. J.; LINS, P. C.; MOURA, S. M.; BATISTA, J. A. N.; SILVA, M. C. M. da; ENGLER, G.; MORGANTE, C. V.; LISEI-DE-SA, M. E.; VASQUES, R. M.; ALMEIDA-ENGLER, J. de; SA, M. F. G. de.
Afiliação: | RODRIGO DA ROCHA FRAGOSO, CNPAE; FABRICIO BARBOSA MONTEIRO ARRAES; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; VÍVIAN JESUS MIRANDA; MARCOS FERNANDO BASSO; ANDRÉ VINICIUS JÚLIO FERREIRA; ANTÔNIO AMÉRICO BARBOSA VIANA; CAMILA BARROZO JESUS LINS; PHILIPPE CASTRO LINS; STÉFANIE MENEZES MOURA; JOÃO AGUIAR NOGUEIRA BATISTA, Federal University of Minas Gerais; MARIA CRISTINA MATTAR DA SILVA, Cenargen; GILBERT ENGLER, Université Côte d'Azur, France; CAROLINA VIANNA MORGANTE, CPATSA; MARIA EUGÊNIA LISEI-DE-SA, Minas Gerais Agricultural Research Company; RAQUEL MEDEIROS VASQUES; JANICE DE ALMEIDA-ENGLER, Université Côte d'Azur, France; MARIA FATIMA GROSSI DE SA, Cenargen.
Título: | Functional characterization of the pUceS8.3 promoter and its potential use for ectopic gene overexpression.
Ano de publicação: | 2022
Fonte/Imprenta: | Planta, v. 256, n. 4, 2022.
DOI: | https://doi.org/10.1007/s00425-022-03980-6
Idioma: | Inglês
Notas: | Na publicação: Rodrigo Rocha Fragoso; Isabela Tristan Lourenço-Tessutti; Maria Cristina Mattar Silva; Maria Fatima Grossi-de-Sa.
Conteúdo: | Soybean (Glycine max) is one of the most important agricultural commodities worldwide and a major protein and oil source. Herein, we identified the soybean ubiquitin-conjugating (E2) enzyme gene (GmUBC4; Glyma.18G216000), which is significantly upregulated in response to Anticarsia gemmatalis attack and Meloidogyne incognita-induced galls during plant parasitism by plant nematode. The GmUBC4 promoter sequence and its different modules were functionally characterized in silico and in planta using transgenic Arabidopsis thaliana and G. max lines. Its full-length transcriptional regulatory region (promoter and 5´-UTR sequences, named pUceS8.3 promoter) was able to drive higher levels of uidA (?-glucuronidase) gene expression in different tissues of transgenic A. thaliana lines compared to its three shortened modules and the p35SdAMV promoter. Notably, higher ?-glucuronidase (GUS) enzymatic activity was shown in M. incognita-induced giant cells when the full pUceS8.3 promoter drove the expression of this reporter gene. Furthermore, nematode-specific dsRNA molecules were successfully overexpressed under the control of the pUceS8.3 promoter in transgenic soybean lines. The RNAi gene construct used here was designed to post-transcriptionally downregulate the previously characterized pre-mRNA splicing factor genes from Heterodera glycines and M. incognita. A total of six transgenic soybean lines containing RNAi gene construct were selected for molecular characterization after infection with M. incognita pre-parasitic second-stage (ppJ2) nematodes. A strong reduction in the egg number produced by M. incognita after parasitism was observed in those transgenic soybean lines, ranging from 71 to 92% compared to wild-type control plants. The present data demonstrated that pUceS8.3 is a gene promoter capable of effectively driving dsRNA overexpression in nematode-induced giant cells of transgenic soybean lines and can be successfully applied as an important biotechnological asset to generate transgenic crops with improved resistance to root-knot nematodes as well as other pests.
Palavras-Chave: | GmUBC4 promoter/gene; Ubiquitin-conjugating (E2) enzyme gene promoter.
Thesagro: | Anticarsia Gemmatalis; Glycine Max; Meloidogyne Incognita; Soja.
Thesaurus Nal: | RNA interference; Soybeans.

Categoria do assunto:

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G Melhoramento Genético

Registro original: | Embrapa Semiárido (CPATSA)

Exemplares:

CENARGEN - (38927-1) - UPC - AP - DD -  -  -  - DIS
CNPAE - (4112-1) - UPC - AP - DD -  -  -  - DIS
CPATSA - (60183-1) - UPC - AP - DD -  -  -  - DIS