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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
12/08/2013 |
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Data da última atualização: |
13/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CHOZE, R.; ALCANTARA, G. B.; ALVES FILHO, E. de G.; SILVA, L. M. A. e; FARIA, J. C.; LIÃO, L. M. |
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Afiliação: |
RAFAEL CHOZE, UFG; GLAUCIA B. ALCANTARA, UFG; ELENILSON DE G. ALVES FILHO, UFG; LORENA MARA A. E SILVA, UFG; JOSIAS CORREA DE FARIA, CNPAF; LUCIANO M. LIÃO, UFG. |
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Título: |
Distinction between a transgenic and a conventional common bean genotype by 1H HR-MAS NMR. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Food Chemistry, v. 141, p. 2841-287, 2013. |
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ISSN: |
0308-8146 |
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DOI: |
http://dx.doi.org/10.1016/j.foodchem.2013.05.123 |
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Idioma: |
Inglês |
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Conteúdo: |
In this work, it is proposed a methodology which allows to differentiate a conventional and a specific transgenic common beans, grown in greenhouse or under field conditions, based on modifications in chemical composition using 1H HR-MAS NMR. It is demonstrated that the influence of typical variables from field planting conditions had no significant influence on the ability to set apart transgenic from conventional. This methodology was corroborated by multivariate data analysis of the 1H NMR and IR spectra. This study also points out the simplicity of using the HR-MAS NMR technique for food analyses. The measurement is highly simplified because it does not require any pretreatment of the sample apart from the addition of a small amount of D2O necessary to produce homogeneous dough and a field frequency lock. Moreover, due to the high concentration of the sample, measurement time in HR-MAS NMR is very short. |
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Palavras-Chave: |
HR-MAS NMR. |
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Thesagro: |
Biotecnologia; Engenharia genética; Feijão; Organismo transgênico; Phaseolus vulgaris. |
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Thesaurus NAL: |
Beans; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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024 7 $ahttp://dx.doi.org/10.1016/j.foodchem.2013.05.123$2DOI
100 1 $aCHOZE, R.
245 $aDistinction between a transgenic and a conventional common bean genotype by 1H HR-MAS NMR.$h[electronic resource]
260 $c2013
520 $aIn this work, it is proposed a methodology which allows to differentiate a conventional and a specific transgenic common beans, grown in greenhouse or under field conditions, based on modifications in chemical composition using 1H HR-MAS NMR. It is demonstrated that the influence of typical variables from field planting conditions had no significant influence on the ability to set apart transgenic from conventional. This methodology was corroborated by multivariate data analysis of the 1H NMR and IR spectra. This study also points out the simplicity of using the HR-MAS NMR technique for food analyses. The measurement is highly simplified because it does not require any pretreatment of the sample apart from the addition of a small amount of D2O necessary to produce homogeneous dough and a field frequency lock. Moreover, due to the high concentration of the sample, measurement time in HR-MAS NMR is very short.
650 $aBeans
650 $aTransgenic plants
650 $aBiotecnologia
650 $aEngenharia genética
650 $aFeijão
650 $aOrganismo transgênico
650 $aPhaseolus vulgaris
653 $aHR-MAS NMR
700 1 $aALCANTARA, G. B.
700 1 $aALVES FILHO, E. de G.
700 1 $aSILVA, L. M. A. e
700 1 $aFARIA, J. C.
700 1 $aLIÃO, L. M.
773 $tFood Chemistry$gv. 141, p. 2841-287, 2013.
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