Data/hora: 04/06/2024 03:09:35

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 08/01/1997
Data da última atualização: | 22/06/2023
Autoria: | BAR-SAGIE, D.; MAYEVSKY, A.; BARTOOV, B.
Título: | A fluorometric-reflectometric technique for simultaneous measurement of pH and motility in ram semen.
Ano de publicação: | 1981
Fonte/Imprenta: | Archivos of Andrology, v. 7, n. 1, p. 27-33, Aug. 1981.
DOI: | 10.3109/01485018109009372.
Idioma: | Inglês
Conteúdo: | Abstract: An optical technique for simultaneous assessment of pH and motility in ram semen was described. Motility was evaluated by the measurement of changes in intensity of reflected light from semen samples illuminated with 366 nm light. pH was measured by using a pH-sensitive fluorescent indicator (umbelliferone). From the changes in fluorescence intensity (450 nm) as a function of the pH of the semen, a calibration curve was constructed. The relationship between pH changes and motility in various metabolic conditions were described. Different kinetics for pH changes occurred when sperm motility was driven by both mitochondrial respiration and fructolysis or by either one of them. An analysis of the fluorescence signal from extracellular or cellular fractions of semen suggested that the pH measurement reflected extracellular pH.
Palavras-Chave: | Analysis; Bioquimica seminal; Hydrogen-Ion Concentration; Motilidade.
Thesagro: | Ovino; Reprodução; Sêmen.
Thesaurus Nal: | Males; Reproduction; Sheep; Sperm motility; Umbelliferones.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (9780-1) - ADD - AP - DD -  -  -  - DIS