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| Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
14/03/2011 |
Data da última atualização: |
14/03/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ALMEIDA, J. D. de; BARROS, L. M. G.; SANTOS, D. B. M.; COTTA, M. G.; BARBOSA, E. A.; CAÇÃO, S. B.; EIRA, M. T. S. da; ALVES, G. S. C.; VINECKY, F.; PEREIRA, L. F. P.; SILVA, F. R. da; ANDRADE, A. C.; MARRACCINI, P.; CARNEIRO, M. |
Afiliação: |
JULIANA DANTAS DE ALMEIDA, CENARGEN; LEILA MARIA GOMES BARROS, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; LUIZ FILIPE PROTASIO PEREIRA, SAPC; ALAN CARVALHO ANDRADE, CENARGEN; CIRAD UMR-DAP; MAURO CARNEIRO, CENARGEN. |
Título: |
Prospection of tissue specific promoters in coffee. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil. |
Idioma: |
Inglês |
Conteúdo: |
The majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented. MenosThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its resp... Mostrar Tudo |
Palavras-Chave: |
Coffee. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02510nam a2200277 a 4500 001 1880640 005 2011-03-14 008 2008 bl uuuu u00u1 u #d 100 1 $aALMEIDA, J. D. de 245 $aProspection of tissue specific promoters in coffee.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil.$c2008 520 $aThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented. 653 $aCoffee 700 1 $aBARROS, L. M. G. 700 1 $aSANTOS, D. B. M. 700 1 $aCOTTA, M. G. 700 1 $aBARBOSA, E. A. 700 1 $aCAÇÃO, S. B. 700 1 $aEIRA, M. T. S. da 700 1 $aALVES, G. S. C. 700 1 $aVINECKY, F. 700 1 $aPEREIRA, L. F. P. 700 1 $aSILVA, F. R. da 700 1 $aANDRADE, A. C. 700 1 $aMARRACCINI, P. 700 1 $aCARNEIRO, M.
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Embrapa Café (CNPCa) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Uva e Vinho. Para informações adicionais entre em contato com cnpuv.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
17/11/2010 |
Data da última atualização: |
11/06/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
RUFATO, A. de R.; TOMAZ, Z. F. P.; GONÇALVES, C. X.; SILVEIRA, S. S.; RUFATO, L.; GOMES, F. R. C. |
Afiliação: |
ANDREA DE ROSSI RUFATO, CNPUV; Z. F. P.TOMAZ, UFPEL; C. X. GONÇALVES, UFPEL; S. S. SILVEIRA, UFPEL; LEO RUFATO, UDESC; FERNANDO ROGERIO COSTA GOMES, CPACT. |
Título: |
The effect of intergrafting on fruit quality and productivity of peach. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Acta Horticulturae, The Hague, n. 872, p. 349-352, ago. 2010. |
Idioma: |
Inglês |
Notas: |
Trabalho apresentado no VIII International Symposium on Temperate Zone Fruits in the Tropics and Subtropics, Florianópolis, 2007. |
Conteúdo: |
Researchs concerning peach rootstocks in Brazil have still not given response to the numerable requests for reduction of plant vigor, allowing a more condense plantation. Intergraft is a practice that can be used to reduce exceeding vigor, when rootstocks are not available to accomplish this purpose. Intending to evaluate the outcome of intergraft on fruit quality and productivity of peach trees, an experiment was set in 2002 in a didactic orchard at ?Centro Agropecuário da Palma? of Universidade Federal de Pelotas? (Brazil). The cultivar ?Jubileu? was used intergrafted with 5, 10, 15 and 20 cm of the cultivar ?Granada? plus a control group (non-intergrafted trees). The rootstock ?Capdeboscq? was raised from seed germination. The intergraft and the scion cultivar were grafted onto the rootstock by budding technique in January and July 2001, respectively. The parameters evaluated at harvest time of the crop 2006 were: yield per hectare, average fruit weight, fruit firmness, total soluble solids, epidermis coloration and fruit grading. The average fruit weight and fruit grading were not meaningfully influenced by the different intergraft lengths. The yield per hectare, fruit firmness and total soluble solids were influenced by the different intergraft lengths. The 20-cm long intergraft showed the lowest yield per hectare. Trees with 20-cm long intergraft produced higher fruit firmness and total soluble solids. Both parameters could be represented by exponential functions: the fruit firmness had a minimum point at 7 cm and total soluble solids had a minimum point at 9.3 cm of intergraft length. The yield per hectare could be represented by a linear decreasing curve. The intergraft technique is positive for quality fruits for ?Jubileu? plants, without downside outcomes in the productivity and classification fruit. MenosResearchs concerning peach rootstocks in Brazil have still not given response to the numerable requests for reduction of plant vigor, allowing a more condense plantation. Intergraft is a practice that can be used to reduce exceeding vigor, when rootstocks are not available to accomplish this purpose. Intending to evaluate the outcome of intergraft on fruit quality and productivity of peach trees, an experiment was set in 2002 in a didactic orchard at ?Centro Agropecuário da Palma? of Universidade Federal de Pelotas? (Brazil). The cultivar ?Jubileu? was used intergrafted with 5, 10, 15 and 20 cm of the cultivar ?Granada? plus a control group (non-intergrafted trees). The rootstock ?Capdeboscq? was raised from seed germination. The intergraft and the scion cultivar were grafted onto the rootstock by budding technique in January and July 2001, respectively. The parameters evaluated at harvest time of the crop 2006 were: yield per hectare, average fruit weight, fruit firmness, total soluble solids, epidermis coloration and fruit grading. The average fruit weight and fruit grading were not meaningfully influenced by the different intergraft lengths. The yield per hectare, fruit firmness and total soluble solids were influenced by the different intergraft lengths. The 20-cm long intergraft showed the lowest yield per hectare. Trees with 20-cm long intergraft produced higher fruit firmness and total soluble solids. Both parameters could be represented by exponential functions: the ... Mostrar Tudo |
Palavras-Chave: |
Firmeza do fruto; Pelotas; Porta-enxerto; Rio Grande do Sul; Vigorisidade. |
Thesagro: |
Enxerto; Fruticultura; Pêssego. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02756naa a2200289 a 4500 001 1867116 005 2019-06-11 008 2010 bl uuuu u00u1 u #d 100 1 $aRUFATO, A. de R. 245 $aThe effect of intergrafting on fruit quality and productivity of peach.$h[electronic resource] 260 $c2010 500 $aTrabalho apresentado no VIII International Symposium on Temperate Zone Fruits in the Tropics and Subtropics, Florianópolis, 2007. 520 $aResearchs concerning peach rootstocks in Brazil have still not given response to the numerable requests for reduction of plant vigor, allowing a more condense plantation. Intergraft is a practice that can be used to reduce exceeding vigor, when rootstocks are not available to accomplish this purpose. Intending to evaluate the outcome of intergraft on fruit quality and productivity of peach trees, an experiment was set in 2002 in a didactic orchard at ?Centro Agropecuário da Palma? of Universidade Federal de Pelotas? (Brazil). The cultivar ?Jubileu? was used intergrafted with 5, 10, 15 and 20 cm of the cultivar ?Granada? plus a control group (non-intergrafted trees). The rootstock ?Capdeboscq? was raised from seed germination. The intergraft and the scion cultivar were grafted onto the rootstock by budding technique in January and July 2001, respectively. The parameters evaluated at harvest time of the crop 2006 were: yield per hectare, average fruit weight, fruit firmness, total soluble solids, epidermis coloration and fruit grading. The average fruit weight and fruit grading were not meaningfully influenced by the different intergraft lengths. The yield per hectare, fruit firmness and total soluble solids were influenced by the different intergraft lengths. The 20-cm long intergraft showed the lowest yield per hectare. Trees with 20-cm long intergraft produced higher fruit firmness and total soluble solids. Both parameters could be represented by exponential functions: the fruit firmness had a minimum point at 7 cm and total soluble solids had a minimum point at 9.3 cm of intergraft length. The yield per hectare could be represented by a linear decreasing curve. The intergraft technique is positive for quality fruits for ?Jubileu? plants, without downside outcomes in the productivity and classification fruit. 650 $aEnxerto 650 $aFruticultura 650 $aPêssego 653 $aFirmeza do fruto 653 $aPelotas 653 $aPorta-enxerto 653 $aRio Grande do Sul 653 $aVigorisidade 700 1 $aTOMAZ, Z. F. P. 700 1 $aGONÇALVES, C. X. 700 1 $aSILVEIRA, S. S. 700 1 $aRUFATO, L. 700 1 $aGOMES, F. R. C. 773 $tActa Horticulturae, The Hague$gn. 872, p. 349-352, ago. 2010.
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