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Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
14/03/2011 |
Data da última atualização: |
14/03/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ALMEIDA, J. D. de; BARROS, L. M. G.; SANTOS, D. B. M.; COTTA, M. G.; BARBOSA, E. A.; CAÇÃO, S. B.; EIRA, M. T. S. da; ALVES, G. S. C.; VINECKY, F.; PEREIRA, L. F. P.; SILVA, F. R. da; ANDRADE, A. C.; MARRACCINI, P.; CARNEIRO, M. |
Afiliação: |
JULIANA DANTAS DE ALMEIDA, CENARGEN; LEILA MARIA GOMES BARROS, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; LUIZ FILIPE PROTASIO PEREIRA, SAPC; ALAN CARVALHO ANDRADE, CENARGEN; CIRAD UMR-DAP; MAURO CARNEIRO, CENARGEN. |
Título: |
Prospection of tissue specific promoters in coffee. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil. |
Idioma: |
Inglês |
Conteúdo: |
The majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented. MenosThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its resp... Mostrar Tudo |
Palavras-Chave: |
Coffee. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02510nam a2200277 a 4500 001 1880640 005 2011-03-14 008 2008 bl uuuu u00u1 u #d 100 1 $aALMEIDA, J. D. de 245 $aProspection of tissue specific promoters in coffee.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil.$c2008 520 $aThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented. 653 $aCoffee 700 1 $aBARROS, L. M. G. 700 1 $aSANTOS, D. B. M. 700 1 $aCOTTA, M. G. 700 1 $aBARBOSA, E. A. 700 1 $aCAÇÃO, S. B. 700 1 $aEIRA, M. T. S. da 700 1 $aALVES, G. S. C. 700 1 $aVINECKY, F. 700 1 $aPEREIRA, L. F. P. 700 1 $aSILVA, F. R. da 700 1 $aANDRADE, A. C. 700 1 $aMARRACCINI, P. 700 1 $aCARNEIRO, M.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
24/04/2024 |
Data da última atualização: |
26/04/2024 |
Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
Autoria: |
OLIVEIRA, A. D. de; CARVALHO, A. M. de; SOUSA, T. R. de; SANTOS, M. V. A. dos; FONSECA, A. C. P. da; JESUS, D. R. de; RIBEIRO, F. P.; DELVICO, F. M. dos S.; MALAQUIAS, J. V.; VEIGA, A. D. |
Afiliação: |
ALEXSANDRA DUARTE DE OLIVEIRA, CPAC; ARMINDA MOREIRA DE CARVALHO, CPAC; THAIS RODRIGUES DE SOUSA; MARCOS VINICIUS ARAÚJO DOS SANTOS; ANA CAROLINE PEREIRA DA FONSECA; DOUGLAS RODRIGEUS DE JESUS; FABIANA PIONTEKOWSKI RIBEIRO; FRANCISCO MARCOS DOS SANTOS DELVICO, CPAC; JUACI VITORIA MALAQUIAS, CPAC; ADRIANO DELLY VEIGA, CPAC. |
Título: |
Monitoramento das variáveis ambientais em café cultivado com e sem braquiária no Planalto Central Brasileiro. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Planaltina, DF: Embrapa Cerrados, 2024. |
Páginas: |
10 p. |
Série: |
(Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 410). |
ISSN: |
2176-509X |
Idioma: |
Português |
Conteúdo: |
O objetivo do estudo foi quantificar os teores de N, nas formas nítrica (NO3-) e amoniacal (NH4+), monitorar a umidade do solo com base no espaço poroso preenchido por água (EPPA), temperatura do solo e fluxo acumulado de óxido nitroso (N2O) no solo em função do manejo na cultura do café (sequeiro e irrigado), com e sem a presença de braquiária. O estudo foi conduzido na Embrapa Cerrados, Planaltina, DF. |
Palavras-Chave: |
Amônio; Óxido nitroso. |
Thesagro: |
Café; Cultivo de Sequeiro; Irrigação; Nitrato; Nitrogênio. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/266080/1/BPD-410-CPAC-26042024.pdf
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Marc: |
LEADER 01422nam a2200337 a 4500 001 2163884 005 2024-04-26 008 2024 bl uuuu u0uu1 u #d 022 $a2176-509X 100 1 $aOLIVEIRA, A. D. de 245 $aMonitoramento das variáveis ambientais em café cultivado com e sem braquiária no Planalto Central Brasileiro.$h[electronic resource] 260 $aPlanaltina, DF: Embrapa Cerrados$c2024 300 $a10 p. 490 $a(Embrapa Cerrados. Boletim de Pesquisa e Desenvolvimento, 410). 520 $aO objetivo do estudo foi quantificar os teores de N, nas formas nítrica (NO3-) e amoniacal (NH4+), monitorar a umidade do solo com base no espaço poroso preenchido por água (EPPA), temperatura do solo e fluxo acumulado de óxido nitroso (N2O) no solo em função do manejo na cultura do café (sequeiro e irrigado), com e sem a presença de braquiária. O estudo foi conduzido na Embrapa Cerrados, Planaltina, DF. 650 $aCafé 650 $aCultivo de Sequeiro 650 $aIrrigação 650 $aNitrato 650 $aNitrogênio 653 $aAmônio 653 $aÓxido nitroso 700 1 $aCARVALHO, A. M. de 700 1 $aSOUSA, T. R. de 700 1 $aSANTOS, M. V. A. dos 700 1 $aFONSECA, A. C. P. da 700 1 $aJESUS, D. R. de 700 1 $aRIBEIRO, F. P. 700 1 $aDELVICO, F. M. dos S. 700 1 $aMALAQUIAS, J. V. 700 1 $aVEIGA, A. D.
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