Data/hora: 19/06/2024 02:12:42

Biblioteca(s): 	Embrapa Meio Norte / UEP-Parnaíba.	
Data corrente: 	30/06/1997	
Data da última atualização: 	16/10/2008	
Autoria: 	MOREIRA, R. A.; BARROS, A. C. H.; STEWART, J. C.; PUSZTAI, A.	
Título: 	Isolation and characterization of a lectin from the seeds of Dioclea grandiflora (Mart.).	
Ano de publicação: 	1983	
Fonte/Imprenta: 	Planta, v. 158, p. 63-69, 1983.	
Idioma: 	Inglês	
Conteúdo: 	By a combination of solubility fractionation, afinity and molecular-sieve chromatography, a lectin preparation containing several closely related lectin components of different isoelectric point was isolated from the seeds of Dioclea grandiflora Mart. The lectins showed a carbohydrate specificty for D-mannose (D-glucose)-binding and  had a requirement for the presence of Ca2+ and MN2+. The results of preliminary characterization studies showed that the D. grandiflora lectins had similar properties to those of concanavalin A, the lectin from the seeds of Canavalia ensiformis, a plant also belonging to the tribe Diocleae. Thus the D. grandiflora lectins contained no covalently bound carbohydrate and had an amino-acid composition characterized by a low content of methionine and the virtual absence of cysteine. Above pH 4.8 they had molecular weight of about 100.000, while below pH 3.1 they were dissociated to half-molecules. Between these two pH values there was a fast association-dissociation equilibrium for the two species. In dissociating solvents, three subunits were obtained of the approximate size of 25-26,000, 13-14,000 and 8-9,000. The lectins from C. grandiflora similar to concanavalin A were more distantly related to the lectins obtained from the members of the tribe Vicieae although these were also specific for D-mannose (D-glucose)-binding.	
Palavras-Chave: 	Carboidrato específico; Lectin; Molecular size; Peso molecular.	
Thesagro: 	Lectina.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP)	
Exemplares: 	CPAMN-UEPP - (7856-1) - ADD - SP - -- - SP 090/1997 -  - 1997.00090 - DIS