Data/hora: 08/06/2024 02:14:31

Biblioteca(s): | Embrapa Milho e Sorgo.
Data corrente: | 28/12/2001
Data da última atualização: | 07/06/2018
Autoria: | LOGUERCIO, L. L.; SANTOS, C. G.; BARRETO, M. R.; GUIMARAES, C. T.; PAIVA, E.
Afiliação: | CLAUDIA TEIXEIRA GUIMARAES, CNPMS.
Título: | Association of PCR and feeding bioassays as a large-scale method to screen tropical Bacillus thuringiensis isolates for a cry constitution with higher insecticidal effect against Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae.
Ano de publicação: | 2001
Fonte/Imprenta: | Letters in Applied Microbiology, Oxford, v. 32, n. 5, p. 362-367, 2001.
Idioma: | Inglês
Conteúdo: | Aims: To verify whether the presence of any of the cry1C. 1D, 1E and 1F genes could be associated with high toxicity against fall armyworm. Methods and Results: A sample of 60 strains from a large collections of tropical Bacillus thuringiensis (B.t.) isolates was subjected to feeding bioassay and gene-specific PCR. Positive amplification of cry-specific fragments, so confirmed by sequencing, revealed that cry1C was ubiquitous and distributed among high and low mortality classes, cry1D was underrepresented and showed no clear association to high toxicity, and cry1F was not detected. The presence of cry1E significantly correlated to high levels of insecticidal activity, as estimated by linear regression analysis. Conclusions: The PCR amplification of cry1E-specific fragments alone appears to be sufficient to identify B.t. strains with high mortality levels against tropical armyworm. Significance and Impact of the Study: The approach presented is promising as a simple and efficient method for first-tier, marker-assisted screening of environment-specific B.t. germplasm effective in controlling a single target pest.
Palavras-Chave: | Armyworm; Lagarta do cartucho.
Thesagro: | Controle Biológico; Spodoptera Frugiperda.
Thesaurus Nal: | biological control.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (13469-1) - UPC - AP - DD -  -  -  - DIS