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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
25/04/2007 |
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Data da última atualização: |
03/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BATISTA, J. S. S.; HUNGRIA, M.; BARCELLOS, F. G.; FERREIRA, M. C.; MENDES, I. C. |
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Afiliação: |
JESIANE STEFÂNIA SILVA BATISTA, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO; FERNANDO GOMES BARCELLOS; MAGDA CRISTIANI FERREIRA, UEL; IÊDA CARVALHO MENDES. |
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Título: |
Variability in Bradyrhizobium japonicum and B. elkanii seven years after introduction of both the exotic microsymbiont and the soybean host in a cerrados soil. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Microbial Ecology, New York, v. 53, n. 2, p. 270-284, Feb. 2007. |
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ISSN: |
0095-3628 |
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DOI: |
10.1007/s00248-006-9149-2 |
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Idioma: |
Inglês |
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Conteúdo: |
The plasticity of rhizobial genomes is far greater than previously thought, with complex genomic recombination events that may be accelerated by the often stressful environmental conditions of the tropics. This study aimed at evaluating changes in soybean rhizobia due to adaptation to inhospitable environmental conditions (high temperatures, drought, and acid soils) in the Brazilian Cerrados. Both the host plant and combinations of four strains of soybean Bradyrhizobium were introduced in an uncropped soil devoid of rhizobia capable of nodulating soybean. After the third year, seeds were not reinoculated. Two hundred and sixty-three isolates were obtained from nodules of field-grown soybean after the seventh year, and their morphological, physiological, serological, and symbiotic properties determined, followed by genetic analysis of conserved and symbiotic genes. B. japonicum strain CPAC 15 (same serogroup as USDA 123) was characterized as having high saprophytic capacity and competitiveness and by the seventh year represented up to 70% of the cultivable population, in contrast to the poor survival and competitiveness of B. japonicum strain CPAC 7 (same serogroup as CB 1809). In general, adapted strains had increased mucoidy, and up to 43% of the isolates showed no serological reaction. High variability, presumably resulting from the adaptation to the harsh environmental conditions, was verified in rep-PCR (polymerase chain reaction) profiles, being lower in strain CPAC 15, intermediate in B. elkanii, and higher in CPAC 7. Restriction fragment length polymorphism (RFLP)-PCR types of the 16S rDNA corresponded to the following: one type for B. elkanii species, two for B. japonicum, associated to CPAC 15 and CPAC 7, and unknown combinations of profiles. However, when nodC sequences and RFLP-PCR of the nifH region data were considered, only two clusters were observed having full congruence with B. japonicum and B. elkanii species. Combining the results, variability was such that even within a genetically more stable group (such as that of CPAC 15), only 6.4% of the isolates showed high similarity to the inoculant strain, whereas none was similar to CPAC 7. The genetic variability in our study seems to result from a variety and combination of events including strain dispersion, genomic recombination, and horizontal gene transfer. Furthermore, the genetic variability appears to be mainly associated with adaptation, saprophytic capacity, and competitiveness, and not with symbiotic effectiveness, as the similarity of symbiotic genes was higher than that of conserved regions of the DNA. MenosThe plasticity of rhizobial genomes is far greater than previously thought, with complex genomic recombination events that may be accelerated by the often stressful environmental conditions of the tropics. This study aimed at evaluating changes in soybean rhizobia due to adaptation to inhospitable environmental conditions (high temperatures, drought, and acid soils) in the Brazilian Cerrados. Both the host plant and combinations of four strains of soybean Bradyrhizobium were introduced in an uncropped soil devoid of rhizobia capable of nodulating soybean. After the third year, seeds were not reinoculated. Two hundred and sixty-three isolates were obtained from nodules of field-grown soybean after the seventh year, and their morphological, physiological, serological, and symbiotic properties determined, followed by genetic analysis of conserved and symbiotic genes. B. japonicum strain CPAC 15 (same serogroup as USDA 123) was characterized as having high saprophytic capacity and competitiveness and by the seventh year represented up to 70% of the cultivable population, in contrast to the poor survival and competitiveness of B. japonicum strain CPAC 7 (same serogroup as CB 1809). In general, adapted strains had increased mucoidy, and up to 43% of the isolates showed no serological reaction. High variability, presumably resulting from the adaptation to the harsh environmental conditions, was verified in rep-PCR (polymerase chain reaction) profiles, being lower in strain CPAC 15,... Mostrar Tudo |
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Thesagro: |
Rhizobium; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03319naa a2200217 a 4500
001 1469973
005 2017-08-03
008 2007 bl uuuu u00u1 u #d
022 $a0095-3628
024 7 $a10.1007/s00248-006-9149-2$2DOI
100 1 $aBATISTA, J. S. S.
245 $aVariability in Bradyrhizobium japonicum and B. elkanii seven years after introduction of both the exotic microsymbiont and the soybean host in a cerrados soil.
260 $c2007
520 $aThe plasticity of rhizobial genomes is far greater than previously thought, with complex genomic recombination events that may be accelerated by the often stressful environmental conditions of the tropics. This study aimed at evaluating changes in soybean rhizobia due to adaptation to inhospitable environmental conditions (high temperatures, drought, and acid soils) in the Brazilian Cerrados. Both the host plant and combinations of four strains of soybean Bradyrhizobium were introduced in an uncropped soil devoid of rhizobia capable of nodulating soybean. After the third year, seeds were not reinoculated. Two hundred and sixty-three isolates were obtained from nodules of field-grown soybean after the seventh year, and their morphological, physiological, serological, and symbiotic properties determined, followed by genetic analysis of conserved and symbiotic genes. B. japonicum strain CPAC 15 (same serogroup as USDA 123) was characterized as having high saprophytic capacity and competitiveness and by the seventh year represented up to 70% of the cultivable population, in contrast to the poor survival and competitiveness of B. japonicum strain CPAC 7 (same serogroup as CB 1809). In general, adapted strains had increased mucoidy, and up to 43% of the isolates showed no serological reaction. High variability, presumably resulting from the adaptation to the harsh environmental conditions, was verified in rep-PCR (polymerase chain reaction) profiles, being lower in strain CPAC 15, intermediate in B. elkanii, and higher in CPAC 7. Restriction fragment length polymorphism (RFLP)-PCR types of the 16S rDNA corresponded to the following: one type for B. elkanii species, two for B. japonicum, associated to CPAC 15 and CPAC 7, and unknown combinations of profiles. However, when nodC sequences and RFLP-PCR of the nifH region data were considered, only two clusters were observed having full congruence with B. japonicum and B. elkanii species. Combining the results, variability was such that even within a genetically more stable group (such as that of CPAC 15), only 6.4% of the isolates showed high similarity to the inoculant strain, whereas none was similar to CPAC 7. The genetic variability in our study seems to result from a variety and combination of events including strain dispersion, genomic recombination, and horizontal gene transfer. Furthermore, the genetic variability appears to be mainly associated with adaptation, saprophytic capacity, and competitiveness, and not with symbiotic effectiveness, as the similarity of symbiotic genes was higher than that of conserved regions of the DNA.
650 $aRhizobium
650 $aSoja
700 1 $aHUNGRIA, M.
700 1 $aBARCELLOS, F. G.
700 1 $aFERREIRA, M. C.
700 1 $aMENDES, I. C.
773 $tMicrobial Ecology, New York$gv. 53, n. 2, p. 270-284, Feb. 2007.
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Embrapa Soja (CNPSO) |
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| Registros recuperados : 68 | |
| 21. |  | BATISTA, J. S. da S.; BARCELLOS, F. G.; MENNA, P.; BALLATI, P. A.; HUNGRIA, M. Confirmação da transferência horizontal de genes simbióticos entre gêneros distintos de rizóbios pela caracterização da estirpe de Sinorhizobium fredii CPAC 402, isolada de nódulo de soja nos cerrados. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira : livro de resumos... Gramado: UFRGS: SBCS, 2007. 1 CD-ROM. Pdf. 7324-1775.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 22. | | BARCELLOS, F. G.; MENNA, P.; BATISTA, J. S. da S.; HUNGRIA, M. Transferência horizontal de genes entre diferentes espécies de rizóbios nos solos dos cerrados. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Soja. |
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| 24. | | BATISTA, J. S. S.; MENNA, P.; BARCELLOS, F. G.; MENDES, I. C.; HUNGRIA, M. Variabilidade de estirpes de Bradyrhizobium japonicum e B. elkanii após sete anos de introdução em um solo dos cerrados. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Soja. |
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| 25. | | BARCELLOS, F. G.; ARAÚJO, T. G. F. de; RODRIGUES, E. P.; HUNGRIA, M. Taxonomic identification and molecular phylogeny of rhizobia isolates that nodulate bean from Paraná soils. In: BRAZILIAN CONGRESS OF GENETICS, 66.; SIMPÓSIO DE CITOGENÉTICA E GENÉTICA DE PEIXES, 19.; REUNIÃO DE GENÉTICA DE MICRORGANISMOS, 32.; CONGRESSO LATINO-AMERICANO DE GENÉTICA PARA CONSERVAÇÃO, 2., 2021, online. Abstracts... Ribeirão Preto: SBG, 2021. e-book. p. 365.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
|  |
| 26. | | BATISTA, J. S. S.; HUNGRIA, M.; BARCELLOS, F. G.; FERREIRA, M. C.; MENDES, I. C. Variability in Bradyrhizobium japonicum and B. elkanii seven years after introduction of both the exotic microsymbiont and the soybean host in a cerrados soil. Microbial Ecology, New York, v. 53, n. 2, p. 270-284, Feb. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 28. | | BORTOLAN, S.; BARCELLOS, F. G.; MARCELINO-GUIMARÃES, F. C.; HUNGRIA, M. Expressão dos genes nodC, nodW e nopP em Bradyrhizobium japonicum estirpe CPAC 15 avaliada por RT-qPCR. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 11, p. 1491-1498, nov. 2009 Título em inglês: Expression of nodC, nodW and nopP genes in Bradyrhizobiumjaponicum CPAC 15 strain evaluated by RT-qPCR.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 30. | | CARVALHO, F. M.; SOUZA, R. C.; BARCELLOS, F. G.; HUNGRIA, M.; VASCONCELOS, A. T. R. Genomic and evolutionary comparisons of diazotrophic and pathogenic bacteria of the order Rhizobiales. BMC Microbiology, London, v. 10, n. 37, p. 1-15, Feb. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 32. | | OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M. The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81. Functional & Integrative Genomics, Heidelberg, v. 10, n. 3, p. 425-431, Aug. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 33. | | MENNA, P.; HUNGRIA, M.; BARCELLOS, F. G.; BANGEL, E. V.; HESS, P. N.; MARTÍNEZ-ROMERO, E. Molecular phylogeny based on the 16S rRNA gene of elite rhizobial strains used in Brazilian commercial inoculants. Systematic and Applied Microbiology, Stuttgart, v.29, n. 4, p. 315-332, Jun. 2006.| Biblioteca(s): Embrapa Soja. |
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| 35. | | MENNA, P.; BARCELLOS, F. G.; BATISTA, J. S. da S.; BANGEL, E.; CAMPO, R. J.; HUNGRIA, M. Análise filogenética de rizóbios, utilizados em inoculantes comerciais brasileiros, com base no sequenciamento do gene ribossomal 16S. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2005, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2005. p.128-133. (Embrapa Soja. Documentos, 268).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 36. | | SOUZA, R. A. de; HUNGRIA, M.; FRANCHINI, J. C.; CHUEIRE. L. M. de O.; BARCELLOS, F. G.; CAMPO, R. J. Avaliação qualitativa e quantitativa da microbiota do solo e da fixação biológica do nitrogênio pela soja. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 1, p. 71-82, jan. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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| 37. | | VESPERO, E. C.; BARCELLOS, F. G.; PENHA, R. E. S.; PERUGINI, M. R. E.; CUNHA, M. H.; SARIDAKIS, H. O. Comparação de métodos moleculares utilizados no estudo epidemiológico de isolados clínicos de Klebsiella pneumoniae produtora de esbl. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24., 2007, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Microbiologia, 2007. 1 CD-ROM. Nome correto do quinto autor HUNGRIA, M.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 38. | | HUNGRIA, M.; MENDES, I. C.; BATISTA, J. S.; BARCELLOS, F. G.; CHUEIRE, L. M. O.; CAMPO, R. J. CPAC 15 ou CPAC 7? eis a questão! In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA (RELARE), 13., 2006, Londrina. Anais... Londrina: Embrapa Soja, 2007. p. 24. (Embrapa Soja. Documentos, 290). Organizado por Rubens José Campo, Mariângela Hungria.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 39. | | HUNGRIA, M.; SOUZA, R. A.; CAMPO, R. J.; FRANCHINI, J. C.; CHUEIRE. L. M. O.; BARCELLOS, F. G. Validação dos parâmetros do protocolo da RELARE recomendados para a avaliação da eficiência agronômica com a cultura da soja. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA (RELARE), 13., 2006, Londrina. Anais... Londrina: Embrapa Soja, 2007. p. 20. (Embrapa Soja. Documentos, 290). Organizado por Rubens José Campo, Mariângela Hungria.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 40. | | BARCELLOS, F. G.; DELAMUTA, J. R. M.; HUNGRIA, M.; MENNA, P.; BATISTA, J. S. da S.; RIBEIRO, R. A. Taxonomia bacteriana - aspectos atuais e perspectivas. In: YAMADA-OGATTA, S. F.; NAKAZATO, G.; FURNALETTO, M. C.; NOGUEIRA, M. A. (Org.). Tópicos especiais em microbiologia. Londrina: UEL, 2015. p. 7-28.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 68 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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