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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
27/06/2017 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, J. M. F; RWAHNIH, M. A.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M. |
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Afiliação: |
João Marcos Fagundes Silva, University of Bras?lia, Bras?lia, DF, Brazil; Maher Al Rwahnih, Department of Plant Pathology, University of California, Davis, CA, USA; Rosana Blawid, University of Bras? ? lia, Bras? ? lia, DF, Brazil; Tatsuya Nagata, University of Bras?lia, Bras?lia, DF, Brazil; THOR VINICIUS MARTINS FAJARDO, CNPUV. |
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Título: |
Discovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Virus Genes, v.53, n. 4, p. 667-671 [online], 2017. |
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DOI: |
10.1007/s11262-017-1470-y |
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Idioma: |
Inglês |
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Conteúdo: |
In this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remark-able identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3 0 untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus . Keywords High-throughput sequencing Virus discovery Luteoviridae Grapevine enamovirus-1 GEV-1 |
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Palavras-Chave: |
GEV-1; Grapevine enamovirus-1; High-throughput sequencing. |
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Thesaurus NAL: |
Luteoviridae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161127/1/GEV1-Virus-Genes-Silva-et-al-published-June-03-2017.pdf
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Marc: |
LEADER 01940naa a2200229 a 4500
001 2071596
005 2019-05-06
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1007/s11262-017-1470-y$2DOI
100 1 $aSILVA, J. M. F
245 $aDiscovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1.$h[electronic resource]
260 $c2017
520 $aIn this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remark-able identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3 0 untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus . Keywords High-throughput sequencing Virus discovery Luteoviridae Grapevine enamovirus-1 GEV-1
650 $aLuteoviridae
653 $aGEV-1
653 $aGrapevine enamovirus-1
653 $aHigh-throughput sequencing
700 1 $aRWAHNIH, M. A.
700 1 $aBLAWID, R.
700 1 $aNAGATA, T.
700 1 $aFAJARDO, T. V. M.
773 $tVirus Genes$gv.53, n. 4, p. 667-671 [online], 2017.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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