Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
08/01/1997 |
Data da última atualização: |
22/06/2023 |
Autoria: |
BAR-SAGIE, D.; MAYEVSKY, A.; BARTOOV, B. |
Título: |
A fluorometric-reflectometric technique for simultaneous measurement of pH and motility in ram semen. |
Ano de publicação: |
1981 |
Fonte/Imprenta: |
Archivos of Andrology, v. 7, n. 1, p. 27-33, Aug. 1981. |
DOI: |
10.3109/01485018109009372. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: An optical technique for simultaneous assessment of pH and motility in ram semen was described. Motility was evaluated by the measurement of changes in intensity of reflected light from semen samples illuminated with 366 nm light. pH was measured by using a pH-sensitive fluorescent indicator (umbelliferone). From the changes in fluorescence intensity (450 nm) as a function of the pH of the semen, a calibration curve was constructed. The relationship between pH changes and motility in various metabolic conditions were described. Different kinetics for pH changes occurred when sperm motility was driven by both mitochondrial respiration and fructolysis or by either one of them. An analysis of the fluorescence signal from extracellular or cellular fractions of semen suggested that the pH measurement reflected extracellular pH. |
Palavras-Chave: |
Analysis; Bioquimica seminal; Hydrogen-Ion Concentration; Motilidade. |
Thesagro: |
Ovino; Reprodução; Sêmen. |
Thesaurus Nal: |
Males; Reproduction; Sheep; Sperm motility; Umbelliferones. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 01691naa a2200301 a 4500 001 1523640 005 2023-06-22 008 1981 bl uuuu u00u1 u #d 024 7 $a10.3109/01485018109009372.$2DOI 100 1 $aBAR-SAGIE, D. 245 $aA fluorometric-reflectometric technique for simultaneous measurement of pH and motility in ram semen.$h[electronic resource] 260 $c1981 520 $aAbstract: An optical technique for simultaneous assessment of pH and motility in ram semen was described. Motility was evaluated by the measurement of changes in intensity of reflected light from semen samples illuminated with 366 nm light. pH was measured by using a pH-sensitive fluorescent indicator (umbelliferone). From the changes in fluorescence intensity (450 nm) as a function of the pH of the semen, a calibration curve was constructed. The relationship between pH changes and motility in various metabolic conditions were described. Different kinetics for pH changes occurred when sperm motility was driven by both mitochondrial respiration and fructolysis or by either one of them. An analysis of the fluorescence signal from extracellular or cellular fractions of semen suggested that the pH measurement reflected extracellular pH. 650 $aMales 650 $aReproduction 650 $aSheep 650 $aSperm motility 650 $aUmbelliferones 650 $aOvino 650 $aReprodução 650 $aSêmen 653 $aAnalysis 653 $aBioquimica seminal 653 $aHydrogen-Ion Concentration 653 $aMotilidade 700 1 $aMAYEVSKY, A. 700 1 $aBARTOOV, B. 773 $tArchivos of Andrology$gv. 7, n. 1, p. 27-33, Aug. 1981.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |