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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
14/02/2011 |
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Data da última atualização: |
19/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. |
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Afiliação: |
MARCOS ROBERTO CHIARATTI, USP/PIRASSUNGA; CHRISTINA RAMIRES FERREIRA, USP/PIRASSUNGA; FELIPE PERECIN, USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; JULIANO RODRIGUES SANGALLI, USP/PIRASSUNUGA; LÍGIA GARCIA MESQUITA, USP/PIRASSUNGA; JÚLIO CÉSAR DE CARVALHO BALIEIRO, USP/PIRASSUNUNGA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL/SAINT-HYACINTHE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL; FLÁVIO VIEIRA MEIRELLES, USP/PIRASSUNUNGA. |
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Título: |
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011. |
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DOI: |
https://doi.org/10.1016/j.rbmo.2010.10.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. MenosOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to ... Mostrar Tudo |
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Palavras-Chave: |
Embryo; Ethidium bromide; Oocyte; Ooplasm; Transfer. |
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Thesaurus Nal: |
mitochondrial DNA. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/876985/1/OoplastMediatedDevelopmental.pdf
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Marc: |
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024 7 $ahttps://doi.org/10.1016/j.rbmo.2010.10.011$2DOI
100 1 $aCHIARATTI, M. R.
245 $aOoplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.$h[electronic resource]
260 $c2011
520 $aOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes.
650 $amitochondrial DNA
653 $aEmbryo
653 $aEthidium bromide
653 $aOocyte
653 $aOoplasm
653 $aTransfer
700 1 $aFERREIRA, C. R.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aSANGALLI, J. R.
700 1 $aMESQUITA, L. G.
700 1 $aBALIEIRO, J. C. de C.
700 1 $aSMITH, L. C.
700 1 $aGARCIA, J. M.
700 1 $aMEIRELLES, F. V.
773 $tReproductive BioMedicine Online$gv. 22, n. 22, p. 172-183, 2011.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 22 | |
| 3. |  | CARVALHO, M. E.; ELER, J. P.; AFAZ, A. L. M.; FERRAZ, J. B. S.; REGITANO, L. C. de A.; MEIRELLES, F. V.; BALIEIRO, J. C. de C. A. Avaliação de diferentes combinações genotípicas para polimorfismo no gene u-calpaína e calpastatina associados com a maciez da carne em bovinos da raça Nelore. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE CARNES, 4., 2007, Campinas, SP. Mercado do Século XXI: qualidade, segurança alimentar, certificação e rastreabilidade - Anais... Campinas: ITAL: CTC, 2007. p. 253-255. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 4. | | CARVALHO, M. E.; ELER, J. P.; BALIEIRO, J. C. de C.; REGITANO, L. C. de A.; MEIRELLES, F. V.; FERRAZ, J. B. S. Associação de polimorfismos no gene da u-calpaína com a maciez da carne em animais da raça Nelore. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 44., Jaboticabal, SP. Anais... Jaboticabal: SBZ: UNESP, 2007. 1 CD-ROM| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | VIZONA, R. G.; PEREZ, B. da C.; PEIXOTO, M. G. C. D.; VIANA, J. H. M.; VENTURA, R. V.; VERCESI FILHO, A. E.; BALIEIRO, J. C. de C. Genetic analysis of in-vitro embryo production traits in Dairy Gir cattle. Theriogenology, v. 148, p. 149-161, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | PEREZ, B. DA C.; PEIXOTO, M. G. C. D.; BRUNELI, F. A. T.; RAMOS, P. V. B.; BALIEIRO, J. C. DE C. Genetic analysis for in vitro embryo production traits in guzerá breed female donors. In: CONGRESO DE LA ASOCIACIÓN LATINO AMERICANA, 24.; CONGRESO DE LA SOCIED CHILENA DE PRODUCIÓN ANIMAL, 40., 2015, Puerto Varas. Anales... Puerto Varas: ALPA, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | VIGNATO, B. S.; COUTINHO, L. L.; CESAR, A. S. M.; POLETI, M. D.; REGITANO, L. C. de A.; BALIEIRO, J. C. de C. Gene co-expression network analysis associated with carcass traits in Nellore steers. In: ANNUAL CONFERENCE OF AUSTRALIAN MARINE SCIENCES ASSOCIATION, 57., 2017, Darwin, Australia. Proceedings... Darwin, Australia: AMSA, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | WENCESLAU, A. A.; LOPES, P. S.; TEODORO, R. L.; BALIEIRO, J. C. de C.; CARNEIRO, P. L. S.; FERREIRA, W. J. Influencia de meio sobre caracteristicas morfologicas em vacas da raca gir leiteiro. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 34., 1997, Juiz de Fora. Anais... Juiz de Fora: SBZ, 1997. p.10-12. v.3| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | RIPAMONTE, P.; MESQUITA, L. G.; CORTEZZI, S. S.; BALIEIRO, J. C. de C.; MERIGHE, G. K. F.; WATANABE, Y. F.; CAETANO, A. R.; MEIRELLES, F. V. Differential gene expression and developmental competence in in vitro produced bovine embryos. Zygote, v. 20, p. 281-290, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | VIGNATO, B. S.; COUTINHO, L. L.; CESAR, A. S. M.; POLETI, M. D.; REGITANO, L. C. de A.; BALIEIRO, J. C. de C. Comparative muscle transcriptome associated with carcass traits of Nellore cattle. BMC Genomics, v. 18, n. 506, p. 1-13, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 13. | | MORAES, M. J.; BARRETO, A. DO N.; AZEVEDO, G. N.; RAMOS, G. G.; PINHO, L. F.; GRUDZINSKI, C. E.; BALIEIRO, J. C. DE C.; GARCIA, A. R. Monitoramento eletrônico do tempo de deslocamento de machos Nelore (bos indicus) em dois sistemas de produção a pasto. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 32., 2023, Natal. Anais... Natal: UFERSA, 2023. p. 232.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | VIGNATO, B. S.; REGITANO, L. C. de A.; COUTINHO, L. L.; CESAR, A. S. M.; POLETI, M. D.; BALIEIRO, J. C. de C. NEDD4: a putative candidate gene for ribeye area in Nellore steers. In: WORKSHOP ON OMICS STRATEGIES APPLIED TO LIVESTOCK SCIENCE, 1., 2017, Piracicaba, SP. Proceedings... São Carlos, SP: Embrapa Pecuária Sudeste, 2017. p. 15. (Embrapa Pecuária Sudeste. Documentos, 125) Editores: Luiz Lehmann Coutinho, ESALQ/USP; Luciana Correia de Almeida Regitano, Embrapa Pecuária Sudeste; Gerson Barreto Mourão, ESALQ/USP; Aline Silva Mello Cesar, ESALQ/USP; Bárbara Silva Vignato, FZEA/USP; Mirele Daiana Poleti,...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 16. | | MORAES, M. J.; PINHO, L. F.; PEZZOPANE, J. R. M.; PEDROSO, A. de F.; BERNARDI, A. C. de C.; OLIVEIRA, P. P. A.; BALIEIRO, J. C. DE C.; GARCIA, A. R. Caracterização de índices de conforto térmico em sistemas de integração lavoura-pecuária-floresta e em pastagens a pleno sol. In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 32., 2023, Natal. Anais... Natal: UFERSA, 2023. p. 233.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 17. | | BOTTA, D.; ARRUDA, R. P. de; WATANABE, Y. F.; BALIEIRO, J. C. de C.; ROMANELLO, N.; BARRETO, A. do N.; PANTOJA, M. H. de A.; GIRO, A.; CARVALHO, C. P. T. de; OLIVEIRA, A. de S.; GARCIA, A. R. Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. Andrologia, v. 51, n. 6, e13266, jul. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | MORAES, M. J.; CASTILHO, E. F. DE; BALIEIRO, J. C. DE C.; BERNARDI, A. C. de C.; BARRETO, A. DO N.; PINHO, L. F.; RAMOS, G. G.; AZEVEDO, G. N.; ZANETTI, L. K.; GARCIA, A. R. Differences in the behavioral parameters of young Zebu and composite bulls kept on Non-Forested or in Integrated Crop–Livestock–Forestry Systems. Animals, v. 14, n. 6, 2024. 19 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 19. | | FERRINHO, A. M.; NASSU, R. T.; ALDAI, N.; UTEMBERG, B. L.; MENDONÇA, F. B.; FURLAN, M. L. N.; BALDI, F.; MUELLER, L. F.; BALIEIRO, J. C. de C.; PEREIRA, A. S. C. Effect of dietary whole cottonseed vitamin e and days of finishing on sensory traits of meat from nellore bulls In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY, 61, 2015, Clermont-Ferrand, France. Proceedings... Clermont-Ferrand, France: INRA, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 22 | |
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