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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
11/12/1995 |
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Data da última atualização: |
24/07/2012 |
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Autoria: |
BALDANI, J. I. |
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Título: |
Curing clover rhizobia of plasmids and defining the ecological and physiological functions of the plasmids. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Texas: Texas A&M University, dez. 1990. 155 p. Tese de Doutorado. |
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Páginas: |
155 p. |
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Série: |
Tese de doutorado |
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Idioma: |
Inglês |
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Notas: |
Tese de Doutorado. Orientada por: Richard W. Weaver. |
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Conteúdo: |
This study was undertaken to define ecological and physiological functions of plasmids in three strains of Rhizobium leguminosarum bv. trifolii. Strains were characterized with respect to the number and size ofresident plasmids. Plasmid profiles indicated the presence of three, four and five plasmids in strains W14-2, W11-9 and W8-7, respectively. Only one plasmid per strain (pRtrW14-2d, pRtrW11-9a and pRtrW8-7c) hybridized with a probe pCHk12 carrying nif genes and therefore was termed psym. The other plasmids were classified as cryptic because of theirunknown functions. Curing of plasmids was accomplished by insertion ofTn5-SacB genes into plasmids and incubation of transconjugants either at 28oC or higher temperature (39oC to 44oC) on 5% sucrose plates.Manycured derivatives were selected simply by incubating transconjugants at 28oC and curing was independent of the size of the plasmid. Other plasmids were cured only with high temperatures. In two cases, pRtrW11-9c and pRtrW11-9c and pRtrW8-7d, plasmids were not eliminated from the cell. Evaluation of the phenotypic expression of cured derivatives in sterile soil subjected to low moisture (-1.5 MPa/28oC) or high temperature (38 to 42oC/-0.03 MPa) indicated that loss of plasmids affected survival of the bacteria. Low soil moisture reduced survival of some derivatives and was observed to be strain dependent. Elimination of plasmids pRtrW14-2a and pRtrw8-7bc increased survival of derivatives underlow soil moisture. High temperature was more detrimental than... MenosThis study was undertaken to define ecological and physiological functions of plasmids in three strains of Rhizobium leguminosarum bv. trifolii. Strains were characterized with respect to the number and size ofresident plasmids. Plasmid profiles indicated the presence of three, four and five plasmids in strains W14-2, W11-9 and W8-7, respectively. Only one plasmid per strain (pRtrW14-2d, pRtrW11-9a and pRtrW8-7c) hybridized with a probe pCHk12 carrying nif genes and therefore was termed psym. The other plasmids were classified as cryptic because of theirunknown functions. Curing of plasmids was accomplished by insertion ofTn5-SacB genes into plasmids and incubation of transconjugants either at 28oC or higher temperature (39oC to 44oC) on 5% sucrose plates.Manycured derivatives were selected simply by incubating transconjugants at 28oC and curing was independent of the size of the plasmid. Other plasmids were cured only with high temperatures. In two cases, pRtrW11-9c and pRtrW11-9c and pRtrW8-7d, plasmids were not eliminated from the cell. Evaluation of the phenotypic expression of cured derivatives in sterile soil subjected to low moisture (-1.5 MPa/28oC) or high temperature (38 to 42oC/-0.03 MPa) indicated that loss of plasmids affected survival of the bacteria. Low soil moisture reduced survival of some derivatives and was observed to be strain dependent. Elimination of plasmids pRtrW14-2a and pRtrw8-7bc increased survival of derivatives underlow soil moisture. High tempe... Mostrar Tudo |
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Palavras-Chave: |
Genetic vector; Rizobio. |
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Thesagro: |
Ecologia; Genética; Plasmídeo; Rhizobium; Vetor. |
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Thesaurus Nal: |
ecology; plasmids. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02272nam a2200253 a 4500
001 1622134
005 2012-07-24
008 1990 bl uuuu m 00u1 u #d
100 1 $aBALDANI, J. I.
245 $aCuring clover rhizobia of plasmids and defining the ecological and physiological functions of the plasmids.
260 $aTexas: Texas A&M University, dez. 1990. 155 p. Tese de Doutorado.$c1990
300 $a155 p.
490 $aTese de doutorado
500 $aTese de Doutorado. Orientada por: Richard W. Weaver.
520 $aThis study was undertaken to define ecological and physiological functions of plasmids in three strains of Rhizobium leguminosarum bv. trifolii. Strains were characterized with respect to the number and size ofresident plasmids. Plasmid profiles indicated the presence of three, four and five plasmids in strains W14-2, W11-9 and W8-7, respectively. Only one plasmid per strain (pRtrW14-2d, pRtrW11-9a and pRtrW8-7c) hybridized with a probe pCHk12 carrying nif genes and therefore was termed psym. The other plasmids were classified as cryptic because of theirunknown functions. Curing of plasmids was accomplished by insertion ofTn5-SacB genes into plasmids and incubation of transconjugants either at 28oC or higher temperature (39oC to 44oC) on 5% sucrose plates.Manycured derivatives were selected simply by incubating transconjugants at 28oC and curing was independent of the size of the plasmid. Other plasmids were cured only with high temperatures. In two cases, pRtrW11-9c and pRtrW11-9c and pRtrW8-7d, plasmids were not eliminated from the cell. Evaluation of the phenotypic expression of cured derivatives in sterile soil subjected to low moisture (-1.5 MPa/28oC) or high temperature (38 to 42oC/-0.03 MPa) indicated that loss of plasmids affected survival of the bacteria. Low soil moisture reduced survival of some derivatives and was observed to be strain dependent. Elimination of plasmids pRtrW14-2a and pRtrw8-7bc increased survival of derivatives underlow soil moisture. High temperature was more detrimental than...
650 $aecology
650 $aplasmids
650 $aEcologia
650 $aGenética
650 $aPlasmídeo
650 $aRhizobium
650 $aVetor
653 $aGenetic vector
653 $aRizobio
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 546 | |
| 9. |  | MARTINS, A.; KIMURA, O.; BALDANI, J. I. Efeito de rizobacterias do grupo Pseudomonas fluorescentes na simbiose de estirpes de Rhizobium com plantas de feijao. In: CONGRESSO LATINO AMERICANO DE CIENCIA DO SOLO, 13.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 1.; SIMPOSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 4., 1996, Aguas de Lindoia-SP. Resumos... Aguas de Lindoia, SP: USP / SLCS / SBCS, 1996. Comissao 3. Trabalho n.98. 1 CD-ROM.| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | VARGAS, L. K.; BALDANI, J. I.; HEMERLY, A. S. Association of rice cultivars with azospirillum brasilense and Burkholderia brasilensis: studies on the expression of ethylene receptors. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SlV.24| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 546 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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