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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
21/10/2022 |
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Data da última atualização: |
30/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, T. G.; ROSMAN, A. C.; GRAVITOL, C.; NOGUEIRA, E. de M.; BALDANI, J. I.; HEMERLY, A. S. |
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Afiliação: |
THAIS LOUISE G. CARVALHO, UFRJ; ALINE CARDOZO ROSMAN, UFRJ; CLICIA GRATIVOL, UENF; EDUARDO DE M. NOGUEIRA, UFRJ; JOSE IVO BALDANI, CNPAB; ADRIANA S. HEMERLY, UFRJ. |
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Título: |
genotypes with contrasting biological nitrogen fixation efficiencies differentially modulate nitrogen metabolism, auxin signaling, and microorganism perception pathways. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Plants, v. 11, n. 15, 1971, 2022. |
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ISSN: |
2223-7747 |
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Idioma: |
Inglês |
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Conteúdo: |
Sugarcane is an economically important crop that is used for the production of fuel ethanol. Diazotrophic bacteria have been isolated from sugarcane tissues, without causing visible plant anatomical changes or disease symptoms. These bacteria can be beneficial to the plant by promoting root growth and an increase in plant yield. Different rates of Biological Nitrogen Fixation (BNF) were observed in different genotypes. The aim of this work was to conduct a comprehensive molecular and physiological analysis of two model genotypes for contrasting BNF efficiency in order to unravel plant genes that are differentially regulated during a natural association with diazotrophic bacteria. A next-generation sequencing of RNA samples from the genotypes SP70-1143 (high-BNF) and Chunee (low-BNF) was performed. A differential transcriptome analysis showed that several pathways were differentially regulated among the two BNF-contrasting genotypes, including nitrogen metabolism, hormone regulation and bacteria recognition. Physiological analyses, such as nitrogenase and GS activity quantification, bacterial colonization, auxin response and root architecture evaluation, supported the transcriptome expression analyses. The differences observed between the genotypes may explain, at least in part, the differences in BNF contributions. Some of the identified genes might be involved in key regulatory processes for a beneficial association and could be further used as tools for obtaining more efficient BNF genotypes. MenosSugarcane is an economically important crop that is used for the production of fuel ethanol. Diazotrophic bacteria have been isolated from sugarcane tissues, without causing visible plant anatomical changes or disease symptoms. These bacteria can be beneficial to the plant by promoting root growth and an increase in plant yield. Different rates of Biological Nitrogen Fixation (BNF) were observed in different genotypes. The aim of this work was to conduct a comprehensive molecular and physiological analysis of two model genotypes for contrasting BNF efficiency in order to unravel plant genes that are differentially regulated during a natural association with diazotrophic bacteria. A next-generation sequencing of RNA samples from the genotypes SP70-1143 (high-BNF) and Chunee (low-BNF) was performed. A differential transcriptome analysis showed that several pathways were differentially regulated among the two BNF-contrasting genotypes, including nitrogen metabolism, hormone regulation and bacteria recognition. Physiological analyses, such as nitrogenase and GS activity quantification, bacterial colonization, auxin response and root architecture evaluation, supported the transcriptome expression analyses. The differences observed between the genotypes may explain, at least in part, the differences in BNF contributions. Some of the identified genes might be involved in key regulatory processes for a beneficial association and could be further used as tools for obtaining more effi... Mostrar Tudo |
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Palavras-Chave: |
Differential RNA seq; Endophytic diazotrophic bacteria; Nitrogen fixing bacteria. |
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Thesaurus Nal: |
Nitrogen metabolism; Transcriptome. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1147564/1/Sugarcane-Genotypes-with-Contrasting-Biological.pdf
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Marc: |
LEADER 02345naa a2200253 a 4500
001 2147564
005 2022-11-30
008 2022 bl uuuu u00u1 u #d
022 $a2223-7747
100 1 $aCARVALHO, T. G.
245 $agenotypes with contrasting biological nitrogen fixation efficiencies differentially modulate nitrogen metabolism, auxin signaling, and microorganism perception pathways.$h[electronic resource]
260 $c2022
520 $aSugarcane is an economically important crop that is used for the production of fuel ethanol. Diazotrophic bacteria have been isolated from sugarcane tissues, without causing visible plant anatomical changes or disease symptoms. These bacteria can be beneficial to the plant by promoting root growth and an increase in plant yield. Different rates of Biological Nitrogen Fixation (BNF) were observed in different genotypes. The aim of this work was to conduct a comprehensive molecular and physiological analysis of two model genotypes for contrasting BNF efficiency in order to unravel plant genes that are differentially regulated during a natural association with diazotrophic bacteria. A next-generation sequencing of RNA samples from the genotypes SP70-1143 (high-BNF) and Chunee (low-BNF) was performed. A differential transcriptome analysis showed that several pathways were differentially regulated among the two BNF-contrasting genotypes, including nitrogen metabolism, hormone regulation and bacteria recognition. Physiological analyses, such as nitrogenase and GS activity quantification, bacterial colonization, auxin response and root architecture evaluation, supported the transcriptome expression analyses. The differences observed between the genotypes may explain, at least in part, the differences in BNF contributions. Some of the identified genes might be involved in key regulatory processes for a beneficial association and could be further used as tools for obtaining more efficient BNF genotypes.
650 $aNitrogen metabolism
650 $aTranscriptome
653 $aDifferential RNA seq
653 $aEndophytic diazotrophic bacteria
653 $aNitrogen fixing bacteria
700 1 $aROSMAN, A. C.
700 1 $aGRAVITOL, C.
700 1 $aNOGUEIRA, E. de M.
700 1 $aBALDANI, J. I.
700 1 $aHEMERLY, A. S.
773 $tPlants$gv. 11, n. 15, 1971, 2022.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 545 | |
| 9. |  | MARTINS, A.; KIMURA, O.; BALDANI, J. I. Efeito de rizobacterias do grupo Pseudomonas fluorescentes na simbiose de estirpes de Rhizobium com plantas de feijao. In: CONGRESSO LATINO AMERICANO DE CIENCIA DO SOLO, 13.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 1.; SIMPOSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 4., 1996, Aguas de Lindoia-SP. Resumos... Aguas de Lindoia, SP: USP / SLCS / SBCS, 1996. Comissao 3. Trabalho n.98. 1 CD-ROM.| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | VARGAS, L. K.; BALDANI, J. I.; HEMERLY, A. S. Association of rice cultivars with azospirillum brasilense and Burkholderia brasilensis: studies on the expression of ethylene receptors. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SlV.24| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 20. | | FERREIRA, J. S.; BALDANI, J. I.; BALDANI, V. L. D. Contribuição da inoculação e reinoculação de Herbaspirillum seropedicae (estirpe BR 11417) na produção de duas cultivares de arroz irrigado. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24.; SIMPÓSIO BRASILEIRO DE MICROBACTÉRIAS, 12.; SIMPÓSIO DE COLEÇÕES DE CULTURA, 2.; ENCONTRO DE ENSINO EM MICROBIOLOGIA, 4., 2007, Brasília, DF. Anais. {São Paulo}: SBM, 2007. 1 p. 1 CD-ROM. Parceria: UFRRJ| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 545 | |
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