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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/09/2021 |
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Data da última atualização: |
28/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS, E. T. de A.; MENESES, C. H. S. G.; VIDAL, M. S.; BALDANI, J. I. |
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Afiliação: |
ELIZABETH TEIXEIRA DE ALMEIDA RAMOS, UFRRJ; CARLOS HENRIQUE SALVINO GADELHA MENESES, UEPB; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Annals of Applied Biology, Annals of Applied Biology, v. 180, p. 163-175. 2022. |
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DOI: |
DOI: 10.1111/aab.12721 |
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Idioma: |
Inglês |
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Conteúdo: |
Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides. MenosGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, th... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial peptide; Bactericidal effect. |
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Thesaurus Nal: |
Biological control; Gluconacetobacter diazotrophicus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02455naa a2200217 a 4500
001 2134566
005 2023-08-28
008 2022 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1111/aab.12721$2DOI
100 1 $aRAMOS, E. T. de A.
245 $aCharacterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans.$h[electronic resource]
260 $c2022
520 $aGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides.
650 $aBiological control
650 $aGluconacetobacter diazotrophicus
653 $aAntimicrobial peptide
653 $aBactericidal effect
700 1 $aMENESES, C. H. S. G.
700 1 $aVIDAL, M. S.
700 1 $aBALDANI, J. I.
773 $tAnnals of Applied Biology, Annals of Applied Biology$gv. 180, p. 163-175. 2022.
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Embrapa Agrobiologia (CNPAB) |
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| 106. |  | FERREIRA, J. S.; VIDEIRA, S. S.; BALDANI, J. I.; BALDANI, V. L. D. Contribuição das bactérias dizatróficas Azospirillum Herpaspirillum na fertilização nitrogenada de plantas. In: REUNION LATINOAMERICANA DE RHIZOBIOLOGIA, 24.; CONFERNCIA IBEROAMERICANA DE NTERACCIONES BENEFICIOSAS MICROORGANISMO -PLANTA-AMBIENTE, 1., del 5 a 8 de mayo de 2009, La Habana, Cuba: Memórias... Havana: [S.n.], 2009. 34| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 110. | | BALDANI, J. I.; BALDANI, V. L. D.; SELDIN, L.; DOBEREINER, J. Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. International Journal of Systematic Bacteriology, Washington, v. 36, p. 86-93, 1986.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 113. | | PERIN, L.; ALVES, G. C.; SIMÕES ARAÚJO, J. L.; BALDANI, J. I.; REIS, V. M. Caracterização de bactérias diazotróficas do gênero Burkholderia em associação com cana-de-açúcar. Seropédica: Embrapa Agrobiologia, 2006. 4 p. Trabalho apresentado na XXVII Reunião Brasileira de Fertilidade do Solo e Nutrição de Plantas, XI Reunião Brasileira sobre Micorrizas, IX Simpósio Brasileiro de Microbiologia do Solo; VI Reunião Brasileira de Biologia do Solo, Bonito/MS,...| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 546 | |
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