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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
16/06/2017 |
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Data da última atualização: |
16/06/2017 |
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Autoria: |
BRAMLEY, T. A.; STIRLING, D.; SWANSTON, I. A.; MENZIES, G. S.; BAIRD, D. T. |
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Título: |
Specific binding sites for LH/chorionic gonadotrophin, low-density lipoprotein, prolactin and FSH in homogenates of human corpus luteum. I: Validation of methods. |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
Journal of Endocrinology, v. 113, n. 2, p. 305-315, May, 1987. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The specific binding of 125I-labelled human chorionic gonadotrophin (hCG), human low-density lipoprotein (hLDL), human FSH (hFSH) and human prolactin (hPRL) to homogenates of human corpus luteum tissue was measured. Specific binding of 125I-labelled hCG was dependent on the temperature and duration of incubation, was inhibited by divalent metal ions or chelating agents, and increased linearly with homogenate concentration. Recovery of bound hormone was more effective using Millipore filtration or polyethylene glycol precipitation compared with centrifugation alone. Binding of 125I-labelled hCG was inhibited specifically by low levels of hCG and human LH (hLH) but not by ovine LH or bovine LH. Incubation of human luteal tissue with ice-cold citrate buffer (pH 3) released more than 90% of specifically bound 125I-labelled hCG within 5 min. This treatment inactivated LH receptors, but did not affect the immunoactivity of hLH released, enabling the measurement of released hormone by radioimmunoassay. Scatchard plots of binding of 125I-labelled LDL to human corpus luteum demonstrated a single class of binding sites. Binding was saturable, increased linearly with increasing concentration of homogenate, and was displaceable by low concentrations of unlabelled LDL. Binding of 125I-labelled hPRL to human luteal homogenates was increased by Mg2+ and was specific for lactogenic hormones (human prolactin, human growth hormone and ovine prolactin). Binding of 125I-labelled hFSH was not dependent on divalent metal ion concentration (in marked contrast to hFSH binding to immature pig granulosa cell receptors) and was displaced by hFSH preparations but not by hPRL, ovine LH or hCG at 1 microgram/ml. These results establish optimal conditions and hormone specificities for the measurement of human luteal gonadotrophin and LDL receptors, and methods for the estimation of hLH/hCG endogenously bound to human corpus luteum tissue. MenosAbstract: The specific binding of 125I-labelled human chorionic gonadotrophin (hCG), human low-density lipoprotein (hLDL), human FSH (hFSH) and human prolactin (hPRL) to homogenates of human corpus luteum tissue was measured. Specific binding of 125I-labelled hCG was dependent on the temperature and duration of incubation, was inhibited by divalent metal ions or chelating agents, and increased linearly with homogenate concentration. Recovery of bound hormone was more effective using Millipore filtration or polyethylene glycol precipitation compared with centrifugation alone. Binding of 125I-labelled hCG was inhibited specifically by low levels of hCG and human LH (hLH) but not by ovine LH or bovine LH. Incubation of human luteal tissue with ice-cold citrate buffer (pH 3) released more than 90% of specifically bound 125I-labelled hCG within 5 min. This treatment inactivated LH receptors, but did not affect the immunoactivity of hLH released, enabling the measurement of released hormone by radioimmunoassay. Scatchard plots of binding of 125I-labelled LDL to human corpus luteum demonstrated a single class of binding sites. Binding was saturable, increased linearly with increasing concentration of homogenate, and was displaceable by low concentrations of unlabelled LDL. Binding of 125I-labelled hPRL to human luteal homogenates was increased by Mg2+ and was specific for lactogenic hormones (human prolactin, human growth hormone and ovine prolactin). Binding of 125I-labelled hFSH ... Mostrar Tudo |
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Palavras-Chave: |
Chorionic Gonadotropin; FSH; LDL; LH; Radioimmunoassay. |
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Thesaurus Nal: |
binding sites; Cattle; Corpus Luteum; Lipoproteins; Metabolism; Prolactin; Sheep; Swine. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02861naa a2200325 a 4500
001 2071025
005 2017-06-16
008 1987 bl uuuu u00u1 u #d
100 1 $aBRAMLEY, T. A.
245 $aSpecific binding sites for LH/chorionic gonadotrophin, low-density lipoprotein, prolactin and FSH in homogenates of human corpus luteum. I$bValidation of methods.
260 $c1987
520 $aAbstract: The specific binding of 125I-labelled human chorionic gonadotrophin (hCG), human low-density lipoprotein (hLDL), human FSH (hFSH) and human prolactin (hPRL) to homogenates of human corpus luteum tissue was measured. Specific binding of 125I-labelled hCG was dependent on the temperature and duration of incubation, was inhibited by divalent metal ions or chelating agents, and increased linearly with homogenate concentration. Recovery of bound hormone was more effective using Millipore filtration or polyethylene glycol precipitation compared with centrifugation alone. Binding of 125I-labelled hCG was inhibited specifically by low levels of hCG and human LH (hLH) but not by ovine LH or bovine LH. Incubation of human luteal tissue with ice-cold citrate buffer (pH 3) released more than 90% of specifically bound 125I-labelled hCG within 5 min. This treatment inactivated LH receptors, but did not affect the immunoactivity of hLH released, enabling the measurement of released hormone by radioimmunoassay. Scatchard plots of binding of 125I-labelled LDL to human corpus luteum demonstrated a single class of binding sites. Binding was saturable, increased linearly with increasing concentration of homogenate, and was displaceable by low concentrations of unlabelled LDL. Binding of 125I-labelled hPRL to human luteal homogenates was increased by Mg2+ and was specific for lactogenic hormones (human prolactin, human growth hormone and ovine prolactin). Binding of 125I-labelled hFSH was not dependent on divalent metal ion concentration (in marked contrast to hFSH binding to immature pig granulosa cell receptors) and was displaced by hFSH preparations but not by hPRL, ovine LH or hCG at 1 microgram/ml. These results establish optimal conditions and hormone specificities for the measurement of human luteal gonadotrophin and LDL receptors, and methods for the estimation of hLH/hCG endogenously bound to human corpus luteum tissue.
650 $abinding sites
650 $aCattle
650 $aCorpus Luteum
650 $aLipoproteins
650 $aMetabolism
650 $aProlactin
650 $aSheep
650 $aSwine
653 $aChorionic Gonadotropin
653 $aFSH
653 $aLDL
653 $aLH
653 $aRadioimmunoassay
700 1 $aSTIRLING, D.
700 1 $aSWANSTON, I. A.
700 1 $aMENZIES, G. S.
700 1 $aBAIRD, D. T.
773 $tJournal of Endocrinology$gv. 113, n. 2, p. 305-315, May, 1987.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
14/01/1998 |
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Data da última atualização: |
18/07/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VASCONCELLOS, C. A.; BARBOSA, J. V. A.; SANTOS, H. L. dos; FRANCA, G. E. de; BAHIA FILHO, A. F. de C. |
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Afiliação: |
EMBRAPA-CNPMS; EMBRAPA-CNPMS. |
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Título: |
Acumulação de massa seca e de nutrientes por dois híbridos de milho com e sem irrigação suplementar. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE MILHO E SORGO, 15., 1984, Maceió. Resumos. Brasília: EMBRAPA-DDT, 1984. p. 104. |
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Série: |
(EPEAL. Documentos, 2). |
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Idioma: |
Português |
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Palavras-Chave: |
Dry matter; Maize; Massa seca; Nutrient; Nutrientes. |
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Thesagro: |
Irrigação; Milho; Zea Mays. |
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Thesaurus NAL: |
corn; irrigation. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53908/1/Acumulacao-massa.pdf
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Marc: |
LEADER 00866nam a2200277 a 4500
001 1478561
005 2018-07-18
008 1984 bl uuuu u00u1 u #d
100 1 $aVASCONCELLOS, C. A.
245 $aAcumulação de massa seca e de nutrientes por dois híbridos de milho com e sem irrigação suplementar.$h[electronic resource]
260 $aIn: CONGRESSO NACIONAL DE MILHO E SORGO, 15., 1984, Maceió. Resumos. Brasília: EMBRAPA-DDT, 1984. p. 104.$c1984
490 $a(EPEAL. Documentos, 2).
650 $acorn
650 $airrigation
650 $aIrrigação
650 $aMilho
650 $aZea Mays
653 $aDry matter
653 $aMaize
653 $aMassa seca
653 $aNutrient
653 $aNutrientes
700 1 $aBARBOSA, J. V. A.
700 1 $aSANTOS, H. L. dos
700 1 $aFRANCA, G. E. de
700 1 $aBAHIA FILHO, A. F. de C.
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Embrapa Milho e Sorgo (CNPMS) |
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