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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
16/06/2017 |
Data da última atualização: |
16/06/2017 |
Autoria: |
TSONIS, C. G.; McNEILLY, A. S.; BAIRD, D. T. |
Título: |
Measurement of exogenous and endogenous inhibin in sheep serum using a new and extremely sensitive bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro. |
Ano de publicação: |
1986 |
Fonte/Imprenta: |
Journal of Endocrinology, v. 110, n. 2, p. 341-352, Aug. 1986. |
DOI: |
10.1677/joe.0.1100341 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: An extremely sensitive and reliable bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro was developed and used to measure exogenous and endogenous inhibin activity in the ewe. The sheep inhibin bioassay is 30- to 40-fold more sensitive than conventional rat inhibin bioassays. The minimum sensitivity of each bioassay in the measurement of inhibin activity in 1 ml of sheep serum is 220 mu. and 4080 mu. in the sheep and rat bioassays respectively. This sensitive inhibin bioassay has permitted, for the first time, the measurement of endogenous inhibin in the peripheral and ovarian vein blood of the sheep, as well as exogenously administered inhibin. The half-life of exogenously administered ovine inhibin (in follicular fluid) in the sheep was calculated as two components (18-24 and 50-60 min) from the inhibin profiles of six ewes. Inhibin contained in the ovine follicular fluid, given as a bolus i.v. injection, increased to maximum levels after 5 min and then remained increased for 10-32 min depending upon the dose administered, before exponentially decaying. The time for inhibin to exert its effect ranged from 3 to 6 h after injection and appeared to be dose-related. The bolus injection of inhibin, apart from causing suppression of FSH, evoked a large rebound increase of FSH up to 400% of preinjection levels. The development of the sheep bioassay will allow the measurement of biologically active inhibin in the peripheral circulation and ovarian vein blood of sheep with the possibility of extending this to man. MenosAbstract: An extremely sensitive and reliable bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro was developed and used to measure exogenous and endogenous inhibin activity in the ewe. The sheep inhibin bioassay is 30- to 40-fold more sensitive than conventional rat inhibin bioassays. The minimum sensitivity of each bioassay in the measurement of inhibin activity in 1 ml of sheep serum is 220 mu. and 4080 mu. in the sheep and rat bioassays respectively. This sensitive inhibin bioassay has permitted, for the first time, the measurement of endogenous inhibin in the peripheral and ovarian vein blood of the sheep, as well as exogenously administered inhibin. The half-life of exogenously administered ovine inhibin (in follicular fluid) in the sheep was calculated as two components (18-24 and 50-60 min) from the inhibin profiles of six ewes. Inhibin contained in the ovine follicular fluid, given as a bolus i.v. injection, increased to maximum levels after 5 min and then remained increased for 10-32 min depending upon the dose administered, before exponentially decaying. The time for inhibin to exert its effect ranged from 3 to 6 h after injection and appeared to be dose-related. The bolus injection of inhibin, apart from causing suppression of FSH, evoked a large rebound increase of FSH up to 400% of preinjection levels. The development of the sheep bioassay will allow the measurement of biologically active inhibin in the peripheral circulation and ... Mostrar Tudo |
Palavras-Chave: |
Biological Assay; Blood supply; Drug effects; FSH; Half-Life; Inhibins; Ovaries. |
Thesaurus Nal: |
Pharmacology; pituitary gland; Rats; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02435naa a2200289 a 4500 001 2070989 005 2017-06-16 008 1986 bl uuuu u00u1 u #d 024 7 $a10.1677/joe.0.1100341$2DOI 100 1 $aTSONIS, C. G. 245 $aMeasurement of exogenous and endogenous inhibin in sheep serum using a new and extremely sensitive bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro. 260 $c1986 520 $aAbstract: An extremely sensitive and reliable bioassay for inhibin based on inhibition of ovine pituitary FSH secretion in vitro was developed and used to measure exogenous and endogenous inhibin activity in the ewe. The sheep inhibin bioassay is 30- to 40-fold more sensitive than conventional rat inhibin bioassays. The minimum sensitivity of each bioassay in the measurement of inhibin activity in 1 ml of sheep serum is 220 mu. and 4080 mu. in the sheep and rat bioassays respectively. This sensitive inhibin bioassay has permitted, for the first time, the measurement of endogenous inhibin in the peripheral and ovarian vein blood of the sheep, as well as exogenously administered inhibin. The half-life of exogenously administered ovine inhibin (in follicular fluid) in the sheep was calculated as two components (18-24 and 50-60 min) from the inhibin profiles of six ewes. Inhibin contained in the ovine follicular fluid, given as a bolus i.v. injection, increased to maximum levels after 5 min and then remained increased for 10-32 min depending upon the dose administered, before exponentially decaying. The time for inhibin to exert its effect ranged from 3 to 6 h after injection and appeared to be dose-related. The bolus injection of inhibin, apart from causing suppression of FSH, evoked a large rebound increase of FSH up to 400% of preinjection levels. The development of the sheep bioassay will allow the measurement of biologically active inhibin in the peripheral circulation and ovarian vein blood of sheep with the possibility of extending this to man. 650 $aPharmacology 650 $apituitary gland 650 $aRats 650 $aSheep 653 $aBiological Assay 653 $aBlood supply 653 $aDrug effects 653 $aFSH 653 $aHalf-Life 653 $aInhibins 653 $aOvaries 700 1 $aMcNEILLY, A. S. 700 1 $aBAIRD, D. T. 773 $tJournal of Endocrinology$gv. 110, n. 2, p. 341-352, Aug. 1986.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
07/01/1999 |
Data da última atualização: |
16/07/2018 |
Autoria: |
MATTOS, J. C. A. de. |
Afiliação: |
JOÃO CARLOS AGUIAR DE MATTOS. |
Título: |
Estudo comparativo entre silagem de milho e a planta seca e desintegrada (Hastes, Folhas e Espigas) na recria e engorda em confinamento de bovinos de corte. |
Ano de publicação: |
1972 |
Fonte/Imprenta: |
Piracicaba: ESALQ, 1972. |
Páginas: |
48 p. |
Idioma: |
Português |
Notas: |
Tese Mestrado. |
Conteúdo: |
Com a finalidade de se comparar o pe de milho na forma de silagem com este seco e triturado alimentou-se durante 148 dias 32 novilhos, 1/2 nelore x guzera divididos em 4 lotes. Conclui-se apos o periodo experimental que havia equivalencia entre os dois alimentos e que seus resultados eram igualmente melhorados com a adicao do referido suplemento proteico. |
Palavras-Chave: |
Alimentacao animal; Animal alimentation; Planta desintegrada; Planta seca; Silagem de milho. |
Thesagro: |
Confinamento; Engorda; Gado de Corte; Milho; Recria; Silagem; Valor Nutritivo. |
Thesaurus NAL: |
beef cattle; feedlots. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01203nam a2200301 a 4500 001 1057135 005 2018-07-16 008 1972 bl uuuu m 00u1 u #d 100 1 $aMATTOS, J. C. A. de 245 $aEstudo comparativo entre silagem de milho e a planta seca e desintegrada (Hastes, Folhas e Espigas) na recria e engorda em confinamento de bovinos de corte. 260 $aPiracicaba: ESALQ$c1972 300 $a48 p. 500 $aTese Mestrado. 520 $aCom a finalidade de se comparar o pe de milho na forma de silagem com este seco e triturado alimentou-se durante 148 dias 32 novilhos, 1/2 nelore x guzera divididos em 4 lotes. Conclui-se apos o periodo experimental que havia equivalencia entre os dois alimentos e que seus resultados eram igualmente melhorados com a adicao do referido suplemento proteico. 650 $abeef cattle 650 $afeedlots 650 $aConfinamento 650 $aEngorda 650 $aGado de Corte 650 $aMilho 650 $aRecria 650 $aSilagem 650 $aValor Nutritivo 653 $aAlimentacao animal 653 $aAnimal alimentation 653 $aPlanta desintegrada 653 $aPlanta seca 653 $aSilagem de milho
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