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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
08/08/2011 |
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Data da última atualização: |
08/08/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAMOS, C. A. N.; ARAUJO, F. R.; SOUZA, I. I. F.; BACANELLI, G.; LUIZ, H. L.; RUSSI, L. S.; OLIVEIRA, R. H. M. de; SOARES, C. O.; ROSINHA, G. M. S.; ALVES, L. C. |
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Afiliação: |
Carlos Alberto do Nascimento Ramos, Bolsista; FLABIO RIBEIRO ARAUJO, CNPGC; Ingrid I.F. Souza, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco; G. Bacanelli, BOLSISTA; Hera L. Luiz, Bolsista; Lívia S. Russi, Bolsista; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC; Leucio C. Alves, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco. |
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Título: |
Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Veterinary Parasitology, v.176, p.79-83, Feb. 2011. Issue 1. |
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Idioma: |
Inglês |
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Conteúdo: |
This paper reports a quantitative real-time polymerase chain reaction (q-PCR) based on the msa2c gene and standardized with Platinum SYBR Green/ROX for the detection of Babesia bovis in cattle. The msa2c q-PCR amplified a DNA fragment with average dissociation temperature of 77.41 ◦C (±0.25 ◦C). No amplification was detected when DNA from B. bigemina, A. marginale or Bos taurus was used as the template. The detection limit of the msa2c q-PCR was 1000 copies per ml of blood sample, with a linear correlation between the number of msa2c copies and threshold cycle. The comparison between msa2c q-PCR and conventional PCR for cytochrome b revealed 88.8% agreement, with a Kappa index of 0.75. In the comparison between msa2c q-PCR and an enzyme-linked immunosorbent assay (ELISA) with semi-purified B. bovis antigen, agreement was 96.3% and the Kappa index was 0.91. The agreement between three tests was 85.8%. The msa2c q-PCR detected a higher number of positive cattle than conventional PCR in an enzootically stable area, but did not differ significantly from ELISA. No significant differences were detected between the three diagnostic tests with cattle from an enzootically unstable area. All animals raised on a tick-free facility were negative for B. bovis in the three tests. These results suggest that msa2c q-PCR is a useful test for the detection of B. bovis infection. |
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Thesagro: |
Babesia Bovis; Babesiose; Sanidade Animal. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02160naa a2200265 a 4500
001 1897432
005 2011-08-08
008 2011 bl uuuu u00u1 u #d
100 1 $aRAMOS, C. A. N.
245 $aReal-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.$h[electronic resource]
260 $c2011
520 $aThis paper reports a quantitative real-time polymerase chain reaction (q-PCR) based on the msa2c gene and standardized with Platinum SYBR Green/ROX for the detection of Babesia bovis in cattle. The msa2c q-PCR amplified a DNA fragment with average dissociation temperature of 77.41 ◦C (±0.25 ◦C). No amplification was detected when DNA from B. bigemina, A. marginale or Bos taurus was used as the template. The detection limit of the msa2c q-PCR was 1000 copies per ml of blood sample, with a linear correlation between the number of msa2c copies and threshold cycle. The comparison between msa2c q-PCR and conventional PCR for cytochrome b revealed 88.8% agreement, with a Kappa index of 0.75. In the comparison between msa2c q-PCR and an enzyme-linked immunosorbent assay (ELISA) with semi-purified B. bovis antigen, agreement was 96.3% and the Kappa index was 0.91. The agreement between three tests was 85.8%. The msa2c q-PCR detected a higher number of positive cattle than conventional PCR in an enzootically stable area, but did not differ significantly from ELISA. No significant differences were detected between the three diagnostic tests with cattle from an enzootically unstable area. All animals raised on a tick-free facility were negative for B. bovis in the three tests. These results suggest that msa2c q-PCR is a useful test for the detection of B. bovis infection.
650 $aBabesia Bovis
650 $aBabesiose
650 $aSanidade Animal
700 1 $aARAUJO, F. R.
700 1 $aSOUZA, I. I. F.
700 1 $aBACANELLI, G.
700 1 $aLUIZ, H. L.
700 1 $aRUSSI, L. S.
700 1 $aOLIVEIRA, R. H. M. de
700 1 $aSOARES, C. O.
700 1 $aROSINHA, G. M. S.
700 1 $aALVES, L. C.
773 $tVeterinary Parasitology$gv.176, p.79-83, Feb. 2011. Issue 1.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/09/2014 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
CRUZ, J. L.; SOUZA, L. F. da S.; QUEIROZ, D. C.; PELACANI, C. R. |
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Afiliação: |
JAILSON LOPES CRUZ, CNPMF; LUIZ FRANCISCO DA SILVA SOUZA, ESALQ; DYANE COELHO QUEIROZ, UFRB; CLAUDINÉIA REGINA PELACANI, UEFS. |
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Título: |
Phosphate application influences the initial growth and accumulation of phosphorus and nitrogen in Umbu (Spondias tuberosa Arruda Camara). |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
International Journal of Agronomy and Agricultural Research, v. 5, n. 1, p. 101-109, 2014. |
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Idioma: |
Inglês |
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Notas: |
Open Access. |
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Conteúdo: |
The objective of the current work is to evaluate the influence of phosphorus (P) on the accumulation of dry matter as well as the nitrogen (N) and P uptake by the umbu. The experiment was conducted under greenhouse conditions and seeds were placed in a 3-liter pot containing Yellow Latosol soil. The experimental design was entirely randomized, employing different P levels (0; 8; 16; 32 and 64 mg kg-1 of soil). The experiment was concluded 108 days post planting, and the characteristics of growth as well as the N and P uptake were evaluated. The P was found to be significant as it promoted an increase in stem diameter, which could improve grafting and establishment of the plants in the field. The least value of Total Dry Mass (TDM) was obtained when the plants were cultivated in the absence of P. Up to the dose of 32 mg of P the increase in the accumulation of Dry Mass by Absorbing Root (DMAR) was slow and gradual; however, between the doses of 32 and 64 mg of P, a substantial accumulation of dry mass in this plant region was observed. The deficiency of P significantly reduced the uptake of P and N, although it did not change the N: P ratio. Phosphorus fertilization allowed greater growth of the xylopodium, local where water and nutrients storage ocurrs; therefore, P fertilization may contribute to increased drought tolerance of this specie. The concept of P-fertilization is highly significant for the growth of the umbu. |
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Thesagro: |
Umbu. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02064naa a2200181 a 4500
001 1994250
005 2023-05-25
008 2014 bl uuuu u00u1 u #d
100 1 $aCRUZ, J. L.
245 $aPhosphate application influences the initial growth and accumulation of phosphorus and nitrogen in Umbu (Spondias tuberosa Arruda Camara).$h[electronic resource]
260 $c2014
500 $aOpen Access.
520 $aThe objective of the current work is to evaluate the influence of phosphorus (P) on the accumulation of dry matter as well as the nitrogen (N) and P uptake by the umbu. The experiment was conducted under greenhouse conditions and seeds were placed in a 3-liter pot containing Yellow Latosol soil. The experimental design was entirely randomized, employing different P levels (0; 8; 16; 32 and 64 mg kg-1 of soil). The experiment was concluded 108 days post planting, and the characteristics of growth as well as the N and P uptake were evaluated. The P was found to be significant as it promoted an increase in stem diameter, which could improve grafting and establishment of the plants in the field. The least value of Total Dry Mass (TDM) was obtained when the plants were cultivated in the absence of P. Up to the dose of 32 mg of P the increase in the accumulation of Dry Mass by Absorbing Root (DMAR) was slow and gradual; however, between the doses of 32 and 64 mg of P, a substantial accumulation of dry mass in this plant region was observed. The deficiency of P significantly reduced the uptake of P and N, although it did not change the N: P ratio. Phosphorus fertilization allowed greater growth of the xylopodium, local where water and nutrients storage ocurrs; therefore, P fertilization may contribute to increased drought tolerance of this specie. The concept of P-fertilization is highly significant for the growth of the umbu.
650 $aUmbu
700 1 $aSOUZA, L. F. da S.
700 1 $aQUEIROZ, D. C.
700 1 $aPELACANI, C. R.
773 $tInternational Journal of Agronomy and Agricultural Research$gv. 5, n. 1, p. 101-109, 2014.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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