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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
18/09/2015 |
Data da última atualização: |
01/03/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, C. G.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; MARTINS, C. F.; BÁO, S. N. |
Afiliação: |
HEIDI CHRISTINA BESSLER CUMPA, CPAC; ALVARO MORAES DA FONSECA NETO, CPAC; CARLOS FREDERICO MARTINS, CPAC; UNB. |
Título: |
Effect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015. |
Idioma: |
Inglês |
Conteúdo: |
Acetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type. MenosAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and... Mostrar Tudo |
Palavras-Chave: |
Cloning; Epigenetic. |
Thesagro: |
Clonagem. |
Thesaurus Nal: |
histone deacetylase. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129960/1/34712.pdf
|
Marc: |
LEADER 03483nam a2200205 a 4500 001 2024379 005 2016-03-01 008 2015 bl uuuu u00u1 u #d 100 1 $aSILVA, C. G. 245 $aEffect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells. 260 $aAnimal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015.$c2015 520 $aAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type. 650 $ahistone deacetylase 650 $aClonagem 653 $aCloning 653 $aEpigenetic 700 1 $aCUMPA, H. C. B. 700 1 $aFONSECA NETO, A. M. da 700 1 $aMARTINS, C. F. 700 1 $aBÁO, S. N.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registros recuperados : 44 | |
1. | | MARTINS, C. F.; BÁO, S. N.; DODE, M. N.; RUMPF, R. Avaliação ultra-estrutural e da fragmentação do dna de espermatozóides bovinos conservados por diferentes tratamentos de liofilização. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 91.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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4. | | MARTINS, C. F.; BÁO, S. N.; DODE, M. N.; CORREA, G. A.; RUMF, R. Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm. Theriogenology, v. 67, p. 1307-1315, 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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7. | | MARTINS, C. F.; DODE, M. N.; BÁO, S. N.; RUMPF, R. Utilização do teste com acridina laranja e túnel para avaliar a integridade dos espermatozóides liofilizados de bovinos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 110.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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10. | | SILVA, C. G.; CUNHA, E. R.; BLUME, G. R.; MALAQUIAS, J. V.; BÁO, S. N.; MARTINS, C. F. Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose. Cryobiology, v. 70, p. 90-94, 2015. p. 90-94Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Cerrados. |
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11. | | ARDISSON-ARAÚJO, D. M. P.; MELO, F. L.; SIHLER, W.; RIBEIRO, B. M.; BÁO, S. N.; SOUZA, M. L. de. Genome organization of Erinnyis ello granulovirus (EeGV), an efficient biopesticide used in Brazilian cassava crops. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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13. | | CUNHA, E. R. da; MARTINS, C. F.; SILVA, G. C.; CUMPA, H. C. B.; BAO, S. N. Effects of prolonged in vitro culture and cryopreservation on viability, DNA Fragmentation, chromosome stability and ultrastructure of bovine cells from amniotic fluid and umbilical cord. Reproduction in Domestic Animals, v. 49, n. 5, p. 806-812, Oct. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Cerrados. |
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16. | | SILVEIRA, E. B. da; CORDEIRO, B. A.; RIBEIRO, B. M.; CASTRO, M. E. B. de C.; SOARES, E. F.; BÁO, S. N. An Anticarsia gemmatalis multiple nucleopolyhedrovirus mutant, v. ApAg, induces hemocytes apoptosis in vivo and displays reduced infectivity in larvae of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae). Virus Research, v. 130, p. 182-192, 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | RICARTE, A. R. F.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; BÁO, S. N.; SILVA, J. B. A. da. Análise molecular e ultraestrutural de espermatozóides caprinos oriundos de animais infectados naturalmente e experimentalmente com o CAEV. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010. 4 f. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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18. | | PARENTE, A. F.; SILVA-PEREIRA, I.; BALDANI, J. I.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T. Construction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein: two potential marker organisms to study bacteria-plant interactions. Canadian Journal of Microbiology, Ottawa, v. 54, p. 786-790, 2008. Parceria: UnB.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Agrobiologia. |
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19. | | SILVA, C. G. da; CUNHA, E. R. da; BÁO, S. N.; BLUME, G. R. dos; OLIVEIRA, R. M. R. de O.; MARTINS, C. F. Criopreservação de sêmen suíno em sistema automatizado comparando diferentes soluções crioprotetoras. In:REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém: SBZ, 2011. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Cerrados. |
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20. | | SILVA, C. G. DA; CUNHA, E. R. DA; BÁO, S. N.; BLUME, G. DOS R.; OLIVEIRA, R. M. R. DE; MARTINS, C. F. Criopreservação de sêmen suíno em sistema automatizado comparando diferentes soluções crioprotetoras. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém. O Desenvolvimento da produção animal e a responsabilidade frente a novos desafios. Belém: Sociedade Brasileira de Zootecnia, 2011. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Cerrados. |
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Registros recuperados : 44 | |
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