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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/11/2020 |
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Data da última atualização: |
29/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; SIDER, L. H.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; LIMA, A. M. C.; DAMASCENO, E. M.; SOUZA, S. C. R.; TEIXEIRA, M. F. da S. |
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Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO; ALICE ANDRIOLI, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; LUCIA HELENA SIDER, CNPC; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO; RENATO MESQUITA PEIXOTO; ANA MILENA CESAR LIMA; EDGAR MARQUES DAMASCENO; SAMARA CRISTINA ROCHA SOUZA; MARIA FÁTIMA DA SILVA TEIXEIRA. |
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Título: |
Vertical transmissibility of small ruminant lentivirus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
PLoSONE, v. 15, n. 11, e0239916, Nov. 2020. |
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DOI: |
https://doi.org/10.1371/journal.pone.0239916 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats. MenosAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in t... Mostrar Tudo |
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Palavras-Chave: |
SRLV. |
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Thesaurus Nal: |
Genetic techniques and protocols; Goat diseases; Goats; Lentivirus; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218347/1/CNPC-2020-Art-40.pdf
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Marc: |
LEADER 02915naa a2200325 a 4500
001 2127175
005 2020-11-29
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0239916$2DOI
100 1 $aARAÚJO, J. F.
245 $aVertical transmissibility of small ruminant lentivirus.$h[electronic resource]
260 $c2020
520 $aAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats.
650 $aGenetic techniques and protocols
650 $aGoat diseases
650 $aGoats
650 $aLentivirus
650 $aSheep diseases
653 $aSRLV
700 1 $aANDRIOLI, A.
700 1 $aPINHEIRO, R. R.
700 1 $aSIDER, L. H.
700 1 $aSOUSA, A. L. M. de
700 1 $aAZEVEDO, D. A. A. de
700 1 $aPEIXOTO, R. M.
700 1 $aLIMA, A. M. C.
700 1 $aDAMASCENO, E. M.
700 1 $aSOUZA, S. C. R.
700 1 $aTEIXEIRA, M. F. da S.
773 $tPLoSONE$gv. 15, n. 11, e0239916, Nov. 2020.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido; Embrapa Uva e Vinho. |
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Data corrente: |
19/11/2009 |
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Data da última atualização: |
26/09/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
TRICHES, W. dos S.; DINIZ, B. C. R.; OLIVEIRA, V. de S.; MARTINS, A. M.; ARAÚJO, A. J. de B.; ALVES, L. A.; PEREIRA, G. E. |
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Afiliação: |
WILLIAN DOS SANTOS TRICHES, CNPq; BRUNA CARLA REIS DINIZ, CNPq; VANESSA DE SOUZA OLIVEIRA, CNPq; ADONILDE MARTA MARTINS; ANA JULIA DE BRITO ARAÚJO; LUIZ ANTONIO ALVES, CPATSA; GIULIANO ELIAS PEREIRA, CNPUV / CPATSA. |
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Título: |
Comparação físico-química de vinhos da variedade Syrah (Vitis vinifera L.) elaborados em dois ciclos no mesmo ano, em região semiarida tropical. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMI-ÁRIDO, 4., 2009, Petrolina. Anais... Petrolina: Embrapa Semi-Árido, 2009. |
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Páginas: |
p. 260-266. |
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Série: |
(Embrapa Semi-Árido. Documentos, 221). |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho teve por objetivo comparar dois vinhos da mesma cultivar de videira (Syrah) elaborados no mesmo ano, no primeiro e no segundo semestre de 2008. O experimento foi realizado em parceria entre a Embrapa Semi-¡rido (Petrolina, PE) e a Fazenda Ouro Verde (Casa Nova,BA). Foram utilizadas plantas instaladas em vinhedo comercial, dispostas em blocos casualizados, onde foram marcadas três repetições de 20 plantas cada. |
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Palavras-Chave: |
Anais; CNPUV; Composição física; Enologia tropical; IC; Iniciação cientifica; Maturação fenólica; Maturação tecnológica; Região semiárida tropical; Syrah; Vinificação. |
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Thesagro: |
Análise Química; Composição Química; Enologia; Uva; Variedade; Vinho. |
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Thesaurus NAL: |
Grapes. |
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Categoria do assunto: |
--
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA-2010/41752/1/OPB2539.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPUV-2010/11978/1/OPB2539.pdf
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Marc: |
LEADER 01755nam a2200421 a 4500
001 1575462
005 2022-09-26
008 2009 bl uuuu u00u1 u #d
100 1 $aTRICHES, W. dos S.
245 $aComparação físico-química de vinhos da variedade Syrah (Vitis vinifera L.) elaborados em dois ciclos no mesmo ano, em região semiarida tropical.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMI-ÁRIDO, 4., 2009, Petrolina. Anais... Petrolina: Embrapa Semi-Árido$c2009
300 $ap. 260-266.
490 $a(Embrapa Semi-Árido. Documentos, 221).
520 $aEste trabalho teve por objetivo comparar dois vinhos da mesma cultivar de videira (Syrah) elaborados no mesmo ano, no primeiro e no segundo semestre de 2008. O experimento foi realizado em parceria entre a Embrapa Semi-¡rido (Petrolina, PE) e a Fazenda Ouro Verde (Casa Nova,BA). Foram utilizadas plantas instaladas em vinhedo comercial, dispostas em blocos casualizados, onde foram marcadas três repetições de 20 plantas cada.
650 $aGrapes
650 $aAnálise Química
650 $aComposição Química
650 $aEnologia
650 $aUva
650 $aVariedade
650 $aVinho
653 $aAnais
653 $aCNPUV
653 $aComposição física
653 $aEnologia tropical
653 $aIC
653 $aIniciação cientifica
653 $aMaturação fenólica
653 $aMaturação tecnológica
653 $aRegião semiárida tropical
653 $aSyrah
653 $aVinificação
700 1 $aDINIZ, B. C. R.
700 1 $aOLIVEIRA, V. de S.
700 1 $aMARTINS, A. M.
700 1 $aARAÚJO, A. J. de B.
700 1 $aALVES, L. A.
700 1 $aPEREIRA, G. E.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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