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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
31/03/1993 |
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Data da última atualização: |
05/03/2024 |
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Autoria: |
RESENDE, R. de O.; HAAN, P. de; VOSSEN, E. van de; AVILA, A. C. de; GOLDBACH, R.; PETERS, D. |
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Afiliação: |
RENATO DE OLIVEIRA RESENDE; PETER DE HAAN; EDWIN VAN DE VOSSEN; ANTONIO CARLOS DE AVILA, CNPH; ROB GOLDBACH; DICK PETERS. |
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Título: |
Defective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Journal of General Virology, v. 73, p. 2509-2516, 1992. |
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DOI: |
https://doi.org/10.1099/0022-1317-73-10-2509 |
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Idioma: |
Inglês |
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Conteúdo: |
Defective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment. MenosDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another conta... Mostrar Tudo |
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Palavras-Chave: |
Caracterizacao; Characterization; DI L RNA; Generation; Geracao; Maintenance; TSWV; Vira-Cabeca. |
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Thesagro: |
Manutenção. |
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Thesaurus Nal: |
Tomato spotted wilt virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02548naa a2200313 a 4500
001 1749565
005 2024-03-05
008 1992 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1099/0022-1317-73-10-2509$2DOI
100 1 $aRESENDE, R. de O.
245 $aDefective interfering L RNA segments of tomato spotted wilt virus retain both virus genome termini and have extensive internal deletions.$h[electronic resource]
260 $c1992
520 $aDefective interfering (DI) RNA molecules derived from the genomic L RNA segment of tomato spotted wilt virus (TSWV) were generated during sequential passage of the virus at high multiplicity. Characterization of DI RNAs from four distinct isolates by Northern blot analysis and sequence determination revealed that both the 5′ and 3′ genomic termini were retained in these molecules. Each DI RNA contained a single internal deletion of approximately 60% to 80% of the L RNA segment. All DI RNAs studied maintain an open reading frame (ORF) which suggests that these defective molecules should be translatable by ribosomes. Detection of only defective molecules with ORFs indicates either that association with ribosomes or translation is a prerequisite for the selection and maintenance of replicating DI RNAs, or that the truncated proteins produced play a role in their selection or replication. Analysis of the junction sites in the DI RNAs showed that short nucleotide sequences are repeated, one at the release and another at the reinitiation point on the L RNA. One of these is lost during the generation of the DI molecules. The presence of repeated sequences at the junction sites seems to be unique for tospovirus DI L RNAs; they have not been described for other DI systems of either positive- or negative-strand RNA viruses. A model for TSWV DI RNA generation is proposed in which the viral polymerase can ‘jump’ across the internal sequences from one secondary structure to another containing the repeated sequences, during the replication of the viral complementary L RNA segment.
650 $aTomato spotted wilt virus
650 $aManutenção
653 $aCaracterizacao
653 $aCharacterization
653 $aDI L RNA
653 $aGeneration
653 $aGeracao
653 $aMaintenance
653 $aTSWV
653 $aVira-Cabeca
700 1 $aHAAN, P. de
700 1 $aVOSSEN, E. van de
700 1 $aAVILA, A. C. de
700 1 $aGOLDBACH, R.
700 1 $aPETERS, D.
773 $tJournal of General Virology$gv. 73, p. 2509-2516, 1992.
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Embrapa Hortaliças (CNPH) |
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| Registros recuperados : 256 | |
| 161. |  | POZZER, L.; NAGATA, T.; LIMA, M. I.; KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de. A new tospovirus naturally infecting cucurbitaceae in Brazil. Fitopatologia Brasileira, Brasilia, v.19, p.321, ago. 1994. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 162. | | BEZERRA, I. C.; LIMA, M. F.; RIBEIRO, S. G.; GIORDANO, L. B.; ZERBINI, F. M.; AVILA, A. C. de. Occurrence of geminivirus in tomato-producing areas in submedio São Francisco. Fitopatologia Brasileira, Brasilia, v. 22, p. 331, ago. 1997. Suplemento.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 163. | | CAMPOS, M. A.; PETERS, J. A.; MELO, P.; AVILA, A. C. de; TORRES, A. C.; MONTE, D. C. Obtencao de batata transgenica cv. Baronesa com resistencia a "Potato Virus Y". Fitopatologia Brasileira, Brasilia, DF, v. 22, p. 333, 1997. Suplemento. Resumo 589. Congresso Brasileiro de Fitopatologia, 30., 1997.| Biblioteca(s): Embrapa Hortaliças. |
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| 164. | | NAGATA, T.; ALVES, D. M. T.; INOUE-NAKATA, A. K.; KITRAGIMA, E. W.; AVILA, A. C. de. Emerging virus on melon: carlavirus or another genus. Virus Reviews & Research, Florianópolis, v. 9, p. 29-30, 2004. Suplement 1. Resumo. Trabalho apresentado no 15º National Meeting of Virology, São Pedro, 2004.| Biblioteca(s): Embrapa Hortaliças. |
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| 166. | | REZENDE, J. A. M.; GALLETI, S. R.; POZZER, L.; RESENDE, R. de O.; AVILA, A. C. de. Incidence, biological and serological characteristics of a tospovirus in experimental fields of zucchini in Sao Paulo State, Brazil. Fitopatologia Brasileira, Brasilia, v.22, n.1, p.92-98, mar. 1997.| Biblioteca(s): Embrapa Hortaliças. |
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| 167. | | AVILA, A. C. de; DELLA VECCHIA, P. T.; LIN, M. T.; OLIVEIRA, L. O. B.; ARAUJO, J. P. Levantamento de viroses infectando melao e melancia na regiao do Submedio Sao Francisco. In: CONGRESSO BRASILEIRO DE OLERICULTURA, 23., 1983, Rio de Janeiro, RJ. Resumos... Rio de Janeiro: SOB, 1983. p.61.| Biblioteca(s): Embrapa Hortaliças. |
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| 168. | | MELO FILHO, P. de A.; NAGATA, T.; DUSI, A. N.; ÁVILA, A. C. de; BUSO, J. A.; RESENDE, R. O. Isolation and sequence of Garlic virus D (GarV-D) associated to garlic plant in Brazil. Virus Reviews & Research, São Paulo, v. 6, n. 2, p. 159, nov. 2001. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 169. | | BOITEUX, L. S.; NAGATA, T.; FONSECA, M. E. M.; GIORDANO, L. de B.; AVILA, A. C. de; KITAJIMA, E. W. Lentilha (Lens culinaris): nova hospedeira natural do tomato spotted wilt virus (TSWV). Horticultura Brasileira, Brasilia, v.11, n.1, p.64, maio 1993. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 172. | | FONSECA, M. E. N.; MARINHO, V. L. A.; KITAJIMA, E. W.; AVILA, A. C. de; DUSI, A. N. Deteccao de RNA tipo viroide de germoplasma de lupulo (Humulus lupulus). Fitopatologia Brasileira, Brasilia, v.14, n.2, p.164, jul. 1989. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 173. | | FONSECA, M. E. N.; BOITEUX, L. S.; AVILA, A. C. de; LIMA, M. I.; KITAJIMA, E. W. Detection of tomato spotted wilt tospovirus in lentil. Plant Disease, St. Paul, v.79, n.3, p.320, 1995. p.320 Resumo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 175. | | BEZERRA, I. C.; RIBEIRO, S. G.; FARIA, J. C.; ZERBINI, F. M.; LIMA, M. F.; AVILA, A. C. de. Distribuição e caracterização de geminivirus no Brasil. In: CONGRESSO BRASILEIRO DE OLERICULTURA, 38., 1998, Petrolina, PE. Anais... Petrolina: Embrapa Semi-Arido, 1999.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Hortaliças. |
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| 176. | | BEZERRA, I. C.; RIBEIRO, S. G.; FARIA, J. C.; ZERBINI, F. M.; LIMA, M. F.; AVILA, A. C. de. Distribuicao e caracterizacao de geminivirus no Brasil. In: CONGRESSO BRASILEIRO DE OLERICULTURA, 38., 1998, Petrolina,PE. Anais... Petrolina: Embrapa Semi-Arido/SOB, 1999. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 178. | | POZZER, L.; NAGATA, T.; LIMA, M. I.; KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de. "Sapeca" an onion disease in the sub-medio Sao Francisco region, Brazil, is caused by a tospovirus with a serologically distinct nucleocapsid protein. Fitopatologia Brasileira, Brasilia, v.19, p.321, ago. 1994. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 179. | | SILVEIRA, F. Q. de P.; ROCHA, H. G. C.; LIMA, M. F.; RESENDE, L. V.; AVILA, A. C. de. A study of the diversity of whitefly-transmited geminivirus in the "submedio" of San Francisco Valley, Brazil. Fitopatologia Brasileira, Brasilia, v.23, p.312, ago. 1998. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 180. | | MACIEL-ZAMBOLIM, E.; COSTA, H.; CAPUCHO, A. S.; ÁVILA, A. C. de; INOUE-NAKATA, A. K.; KITAJIMA, E. W. Surto epidemiológico do vírus do mosaico amarelo do pimentão em tomateiro na região serrana do Espirito Santo. Fitopatologia Brasileira, Brasilia, v. 29, n. 3, p. 325-327, maio/jun. 2004.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 256 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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