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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
10/01/2017 |
Data da última atualização: |
02/03/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
EBERHARDT, I. D. R.; SCHULTZ, B.; RIZZI, R.; SANCHES, I. D.; FORMAGGIO, A. R.; ATZBERGER, C.; MELLO, M. P.; IMMITZER, M.; TRABAQUINI, K.; LUIZ, A. J. B.; FOSCHIERA, W. |
Afiliação: |
ISAQUE DANIEL ROCHA EBERHARDT, UnB; BRUNO SCHULTZ, INPE; RODRIGO RIZZI, UFPel; IEDA DELÁRCO SANCHES, INPE; ANTONIO ROBERTO FORMAGGIO, INPE; CLEMENT ATZBERGER, University of Natural Resources and Life Sciences, Vienna; MARCIO PUPIN MELLO, The Boing Company; MARKUS IMMITZER, University of Natural Resources and Life Sciences, Vienna; KLEBER TRABAQUINI, Epagri; ALFREDO JOSE BARRETO LUIZ, CNPMA; WILLIAM FOSCHIERA, INPE. |
Título: |
Cloud cover assessment for operational crop monitoring systems in tropical areas. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Remote Sensing, v. 8, n. 3, p. 1-14, 2016. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The potential of optical remote sensing data to identify, map and monitor croplands is well recognized. However, clouds strongly limit the usefulness of optical imagery for these applications. This paper aims at assessing cloud cover conditions over four states in the tropical and sub-tropical Center-South region of Brazil to guide the development of an appropriate agricultural monitoring system based on Landsat-like imagery. Cloudiness was assessed during overlapping four months periods to match the typical length of crop cycles in the study area. The percentage of clear sky occurrence was computed from the 1 km resolution MODIS Cloud Mask product (MOD35) considering 14 years of data between July 2000 and June 2014. Results showed high seasonality of cloud occurrence within the crop year with strong variations across the study area. The maximum seasonality was observed for the two states in the northern part of the study area (i.e., the ones closer to the Equator line), which also presented the lowest averaged values (15%) of clear sky occurrence during the main (summer) cropping period (November to February). In these locations, optical data faces severe constraints for mapping summer crops. On the other hand, relatively favorable conditions were found in the southern part of the study region. In the South, clear sky values of around 45% were found and no signi?cant clear sky seasonality was observed. Results underpin the challenges to implement an operational crop monitoring system based solely on optical remote sensing imagery in tropical and sub-tropical regions, in particular if short-cycle crops have to be monitored during the cloudy summer months. To cope with cloudiness issues, we recommend the use of new systems with higher repetition rates such as Sentinel-2. For local studies, Unmanned Aircraft Vehicles(UAVs) might be used to augment the observing capability. Multi-sensor approaches combining optical and microwave data can be another option. In cases where wall-to-wall maps are not mandatory, statistical sampling approaches might also be a suitable alternative for obtaining useful crop area information. MenosAbstract: The potential of optical remote sensing data to identify, map and monitor croplands is well recognized. However, clouds strongly limit the usefulness of optical imagery for these applications. This paper aims at assessing cloud cover conditions over four states in the tropical and sub-tropical Center-South region of Brazil to guide the development of an appropriate agricultural monitoring system based on Landsat-like imagery. Cloudiness was assessed during overlapping four months periods to match the typical length of crop cycles in the study area. The percentage of clear sky occurrence was computed from the 1 km resolution MODIS Cloud Mask product (MOD35) considering 14 years of data between July 2000 and June 2014. Results showed high seasonality of cloud occurrence within the crop year with strong variations across the study area. The maximum seasonality was observed for the two states in the northern part of the study area (i.e., the ones closer to the Equator line), which also presented the lowest averaged values (15%) of clear sky occurrence during the main (summer) cropping period (November to February). In these locations, optical data faces severe constraints for mapping summer crops. On the other hand, relatively favorable conditions were found in the southern part of the study region. In the South, clear sky values of around 45% were found and no signi?cant clear sky seasonality was observed. Results underpin the challenges to implement an operational cr... Mostrar Tudo |
Palavras-Chave: |
Agriculture monitoring; Clear sky coverage; Cloudiness; Crop classification; Imagem de satélite; MODIS; Monitoramento agrícola. |
Thesagro: |
Agricultura; Nuvem; Sensoriamento remoto. |
Thesaurus Nal: |
Remote sensing. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/153098/1/2016AP07.pdf
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Marc: |
LEADER 03189naa a2200373 a 4500 001 2060359 005 2017-03-02 008 2016 bl uuuu u00u1 u #d 100 1 $aEBERHARDT, I. D. R. 245 $aCloud cover assessment for operational crop monitoring systems in tropical areas.$h[electronic resource] 260 $c2016 520 $aAbstract: The potential of optical remote sensing data to identify, map and monitor croplands is well recognized. However, clouds strongly limit the usefulness of optical imagery for these applications. This paper aims at assessing cloud cover conditions over four states in the tropical and sub-tropical Center-South region of Brazil to guide the development of an appropriate agricultural monitoring system based on Landsat-like imagery. Cloudiness was assessed during overlapping four months periods to match the typical length of crop cycles in the study area. The percentage of clear sky occurrence was computed from the 1 km resolution MODIS Cloud Mask product (MOD35) considering 14 years of data between July 2000 and June 2014. Results showed high seasonality of cloud occurrence within the crop year with strong variations across the study area. The maximum seasonality was observed for the two states in the northern part of the study area (i.e., the ones closer to the Equator line), which also presented the lowest averaged values (15%) of clear sky occurrence during the main (summer) cropping period (November to February). In these locations, optical data faces severe constraints for mapping summer crops. On the other hand, relatively favorable conditions were found in the southern part of the study region. In the South, clear sky values of around 45% were found and no signi?cant clear sky seasonality was observed. Results underpin the challenges to implement an operational crop monitoring system based solely on optical remote sensing imagery in tropical and sub-tropical regions, in particular if short-cycle crops have to be monitored during the cloudy summer months. To cope with cloudiness issues, we recommend the use of new systems with higher repetition rates such as Sentinel-2. For local studies, Unmanned Aircraft Vehicles(UAVs) might be used to augment the observing capability. Multi-sensor approaches combining optical and microwave data can be another option. In cases where wall-to-wall maps are not mandatory, statistical sampling approaches might also be a suitable alternative for obtaining useful crop area information. 650 $aRemote sensing 650 $aAgricultura 650 $aNuvem 650 $aSensoriamento remoto 653 $aAgriculture monitoring 653 $aClear sky coverage 653 $aCloudiness 653 $aCrop classification 653 $aImagem de satélite 653 $aMODIS 653 $aMonitoramento agrícola 700 1 $aSCHULTZ, B. 700 1 $aRIZZI, R. 700 1 $aSANCHES, I. D. 700 1 $aFORMAGGIO, A. R. 700 1 $aATZBERGER, C. 700 1 $aMELLO, M. P. 700 1 $aIMMITZER, M. 700 1 $aTRABAQUINI, K. 700 1 $aLUIZ, A. J. B. 700 1 $aFOSCHIERA, W. 773 $tRemote Sensing$gv. 8, n. 3, p. 1-14, 2016.
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Embrapa Meio Ambiente (CNPMA) |
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Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
17/03/2009 |
Data da última atualização: |
12/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
GARCIA, P. M.; ARCURI, E. F.; BRITO, M. A. V. P.; LANGE, C. C.; BRITO, J. R. F.; CERQUEIRA, M. M. O. P. |
Afiliação: |
P. M. Garcia, UFMG; Edna Froeder Arcuri, Embrapa Gado de Leite; Maria Aparecida Vasconcelos Paiva e Brito, Embrapa Gado de Leite; Carla Christine Lange, Embrapa Gado de Leite; José Renaldi Feitosa Brito, Embrapa Gado de Leite; Mônica Maria Oliveira Pinho Cerqueira, UFMG. |
Título: |
Detecção de Escherichia coli O157:H7 inoculada experimentalmente em amostras de leite cru por método convencional e PCR multiplex. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 60, n. 5, p. 1241-1249, 2008. |
DOI: |
https://doi.org/10.1590/S0102-09352008000500029 |
Idioma: |
Português |
Conteúdo: |
RESUMO - Padronizou-se um método de reação em cadeia da polimerase (PCR) multiplex para detecção de Escherichia coli O157:H7 e avaliou-se a eficiência da PCR e de um método de cultivo convencional em placas na detecção desse patógeno experimentalmente adicionado em leite estéril e em leite cru com baixa contagem bacteriana total (média de 4,01 x 10³ UFC/ml) e com alta contagem bacteriana (média de 2,10 x 10(6) UFC/ml). Foram padronizadas duas reações de PCR com o uso dos primers: "A" (RfbF; RfbR e FLICh7F/FLICh7R) e "B" (SLT-IF/SLTIR e SLT-IIF/SLT-IIR). A detecção de E. coli O157:H7 (1UFC/ml) a partir do leite estéril e do leite cru com baixa contaminação bacteriana foi possível quando se utilizou o método de contagem em placas e a PCR. A sensibilidade dos dois métodos foi menor quando se testou o leite cru com alta contaminação microbiana, sendo o método convencional mais sensível. Os resultados indicam que a presença de outros microrganismos, em alta quantidade no leite, dificulta a detecção de E. coli O157:H7 pelos métodos utilizados. ABSTRACT - This experiment was carried out in order to evaluate the effect of the raw milk bacterial count on the efficiency of a multiplex polymerase chain reaction and a conventional plate count method for detection of Escherichia coli O157:H7. This pathogen was experimentally inoculated into sterile milk, raw milk with low bacterial count (count mean of 4.01 x 10³ cfu/ml) and, raw milk with high bacterial count (mean 2.10 x 10(6) cfu/ml). Two protocols of PCR were standardized using primers "A" (Rfbf and Rfbr and FLICh7F/FLICh7R) and "B" (SLT-IF/SLTIR and SLT-IIF/SLT-IIR). Both conventional plate count and PCR methods were able to detect the presence of E. coli O157:H7 in either sterile milk or raw milk with low bacterial count initially inoculated with 1cfu of E. coli O157:H7 per ml. The sensibility of both methods for high-contaminated raw milk samples was lower, being the conventional approach more sensitive. These results indicate that high bacterial count in raw milk can affect E. coli O157:H7 detection. MenosRESUMO - Padronizou-se um método de reação em cadeia da polimerase (PCR) multiplex para detecção de Escherichia coli O157:H7 e avaliou-se a eficiência da PCR e de um método de cultivo convencional em placas na detecção desse patógeno experimentalmente adicionado em leite estéril e em leite cru com baixa contagem bacteriana total (média de 4,01 x 10³ UFC/ml) e com alta contagem bacteriana (média de 2,10 x 10(6) UFC/ml). Foram padronizadas duas reações de PCR com o uso dos primers: "A" (RfbF; RfbR e FLICh7F/FLICh7R) e "B" (SLT-IF/SLTIR e SLT-IIF/SLT-IIR). A detecção de E. coli O157:H7 (1UFC/ml) a partir do leite estéril e do leite cru com baixa contaminação bacteriana foi possível quando se utilizou o método de contagem em placas e a PCR. A sensibilidade dos dois métodos foi menor quando se testou o leite cru com alta contaminação microbiana, sendo o método convencional mais sensível. Os resultados indicam que a presença de outros microrganismos, em alta quantidade no leite, dificulta a detecção de E. coli O157:H7 pelos métodos utilizados. ABSTRACT - This experiment was carried out in order to evaluate the effect of the raw milk bacterial count on the efficiency of a multiplex polymerase chain reaction and a conventional plate count method for detection of Escherichia coli O157:H7. This pathogen was experimentally inoculated into sterile milk, raw milk with low bacterial count (count mean of 4.01 x 10³ cfu/ml) and, raw milk with high bacterial count (mean 2.10 x 10(6) cfu/ml).... Mostrar Tudo |
Palavras-Chave: |
Antígeno H7; Antígeno O157; PCR multiplex. |
Thesagro: |
Leite; Patógeno. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/596259/1/Deteccao-de-Escherichia-coli-O157-H7-inoculada-experimentalmente-em-amostras-de-leite.pdf
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Marc: |
LEADER 02972naa a2200253 a 4500 001 1596259 005 2022-08-12 008 2008 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1590/S0102-09352008000500029$2DOI 100 1 $aGARCIA, P. M. 245 $aDetecção de Escherichia coli O157$bH7 inoculada experimentalmente em amostras de leite cru por método convencional e PCR multiplex.$h[electronic resource] 260 $c2008 520 $aRESUMO - Padronizou-se um método de reação em cadeia da polimerase (PCR) multiplex para detecção de Escherichia coli O157:H7 e avaliou-se a eficiência da PCR e de um método de cultivo convencional em placas na detecção desse patógeno experimentalmente adicionado em leite estéril e em leite cru com baixa contagem bacteriana total (média de 4,01 x 10³ UFC/ml) e com alta contagem bacteriana (média de 2,10 x 10(6) UFC/ml). Foram padronizadas duas reações de PCR com o uso dos primers: "A" (RfbF; RfbR e FLICh7F/FLICh7R) e "B" (SLT-IF/SLTIR e SLT-IIF/SLT-IIR). A detecção de E. coli O157:H7 (1UFC/ml) a partir do leite estéril e do leite cru com baixa contaminação bacteriana foi possível quando se utilizou o método de contagem em placas e a PCR. A sensibilidade dos dois métodos foi menor quando se testou o leite cru com alta contaminação microbiana, sendo o método convencional mais sensível. Os resultados indicam que a presença de outros microrganismos, em alta quantidade no leite, dificulta a detecção de E. coli O157:H7 pelos métodos utilizados. ABSTRACT - This experiment was carried out in order to evaluate the effect of the raw milk bacterial count on the efficiency of a multiplex polymerase chain reaction and a conventional plate count method for detection of Escherichia coli O157:H7. This pathogen was experimentally inoculated into sterile milk, raw milk with low bacterial count (count mean of 4.01 x 10³ cfu/ml) and, raw milk with high bacterial count (mean 2.10 x 10(6) cfu/ml). Two protocols of PCR were standardized using primers "A" (Rfbf and Rfbr and FLICh7F/FLICh7R) and "B" (SLT-IF/SLTIR and SLT-IIF/SLT-IIR). Both conventional plate count and PCR methods were able to detect the presence of E. coli O157:H7 in either sterile milk or raw milk with low bacterial count initially inoculated with 1cfu of E. coli O157:H7 per ml. The sensibility of both methods for high-contaminated raw milk samples was lower, being the conventional approach more sensitive. These results indicate that high bacterial count in raw milk can affect E. coli O157:H7 detection. 650 $aLeite 650 $aPatógeno 653 $aAntígeno H7 653 $aAntígeno O157 653 $aPCR multiplex 700 1 $aARCURI, E. F. 700 1 $aBRITO, M. A. V. P. 700 1 $aLANGE, C. C. 700 1 $aBRITO, J. R. F. 700 1 $aCERQUEIRA, M. M. O. P. 773 $tArquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte$gv. 60, n. 5, p. 1241-1249, 2008.
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