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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/02/2024 |
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Data da última atualização: |
08/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CUNHA, T.; MIRANDA, M. P.; ZANARDI, O. Z.; MONNERAT, R.; MARQUES, J. P. R.; DORTA, S. O.; MACEDO, C. L.; MACHADO, M. A.; ASTUA, J. de F. |
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Afiliação: |
TATIANE CUNHA, UNIVERSIDADE DE SÃO PAULO; MARCELO P. MIRANDA, FUNDO DE DEFESA DA CITRICULTURA (FUNDECITRUS); ODIMAR Z. ZANARDI, INSTITUTO FEDERAL DE SANTA CATARINA; ROSE MONNERAT, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; JOÃO P.R. MARQUES, UNIVERSITY OF SÃO PAULO; SÍLVIA O. DORTA, UNIVERSIDADE DE SÃO PAULO; CRISTINA L. MACEDO, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; MARCOS A. MACHADO, CENTRO DE CITRICULTURA SYLVIO MOREIRA; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Bacillus thuringiensis translocation in citrus scion/rootstock combinations and binding of cry toxins with the Asian citrus psyllid gut receptors. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Journal Pre-proof, Crop Protection, 2024. |
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Páginas: |
46 p. |
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DOI: |
https://doi.org/10.1016/j.cropro.2024.106593 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation in the xylem of small and large citrus plants and the potential of the bacteria to cause ACP nymphs mortality that fed on shoots from Bt-treated citrus. MenosAbstract: Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation ... Mostrar Tudo |
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Thesagro: |
Porta Enxerto; Translocação. |
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Thesaurus Nal: |
Brush border membrane vesicles; Citrus; Diaphorina citri; Endotoxins. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02611naa a2200313 a 4500
001 2161812
005 2024-02-08
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cropro.2024.106593$2DOI
100 1 $aCUNHA, T.
245 $aBacillus thuringiensis translocation in citrus scion/rootstock combinations and binding of cry toxins with the Asian citrus psyllid gut receptors.$h[electronic resource]
260 $c2024
300 $a46 p.
520 $aAbstract: Bacillus thuringiensis (Bt) can be effective in controlling insect pests either in spray products or by their toxins expressed in transgenic crops. The discovery of Bt as endophytic in citrus opened new perspectives for the control of Diaphorina citri Kuwayama, the vector of the bacteria Candidatus Liberibacter spp. associated with Huanglongbing. In this study, the endophytic translocation of Bt in citrus seedlings (small and large plants) and in different scion-rootstock combinations were investigated. We also assessed the lethal concentration of D. citri nymphs caused by systemic Bt in citrus and, moreover, the binding of Cry toxins with the Asian citrus psyllid (ACP) gut receptors. Endophytic translocation of Bt in citrus seedlings and nursery trees was confirmed by colony isolation, PCR detection, and Btk-GFP microscopy analyses, which demonstrated that, after Bt inoculation into the substrate, the bacteria enter through lateral root emergence sites and are localized in stem xylem vessel elements. Mean lethal concentrations for D. citri third instar nymphs were 4.92 × 104 and 2.19 × 104 spores mL−1 for S1450 and S1302 strains, respectively. Nymphal mortality induced by the bacteria was higher in citrus seedlings than in citrus nursery trees. All Cry-toxins tested were able to bind to D. citri brush border membrane vesicles, suggesting the potential pathogenicity of Bt strains against psyllid. This study clearly demonstrates the penetration and Bt translocation in the xylem of small and large citrus plants and the potential of the bacteria to cause ACP nymphs mortality that fed on shoots from Bt-treated citrus.
650 $aBrush border membrane vesicles
650 $aCitrus
650 $aDiaphorina citri
650 $aEndotoxins
650 $aPorta Enxerto
650 $aTranslocação
700 1 $aMIRANDA, M. P.
700 1 $aZANARDI, O. Z.
700 1 $aMONNERAT, R.
700 1 $aMARQUES, J. P. R.
700 1 $aDORTA, S. O.
700 1 $aMACEDO, C. L.
700 1 $aMACHADO, M. A.
700 1 $aASTUA, J. de F.
773 $tJournal Pre-proof, Crop Protection, 2024.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/01/2009 |
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Data da última atualização: |
06/06/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
VALICENTE, F. H.; CARNEIRO, N. P.; UTIDA, R. H.; GUIMARAES, C. T.; MAGALHAES, J. V. de; PAIVA, E.; CARNEIRO, A. A. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS; R. H. Utida; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS; Edilson Paiva, Embrapa Milho e Sorgo; ANDREA ALMEIDA CARNEIRO, CNPMS. |
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Título: |
Transformation of maize Elite lines with cry1ca of Bacillus thuringiensis to control Spodoptera frugiperda. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: PACIFIC RIM CONFERENCE ON BIOTECHNOLOGY OF BACILLUS THURINGIENSIS AND ITS ENVIRONMENT IMPACT, 6., 2005, Victoria. Proceedings. Victoria: Biocontrol Network of Canada: Réseau Biocontrôle du Canada, 2007. p. 81-83. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Fall armyworm; Insect pathogen; Spodopera frugiperda; Transgenic maize. |
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Thesagro: |
Milho. |
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Thesaurus NAL: |
corn. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/59204/1/Transformation-maize.pdf
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Marc: |
LEADER 00906nam a2200241 a 4500
001 1491659
005 2018-06-06
008 2007 bl uuuu u00u1 u #d
100 1 $aVALICENTE, F. H.
245 $aTransformation of maize Elite lines with cry1ca of Bacillus thuringiensis to control Spodoptera frugiperda.$h[electronic resource]
260 $aIn: PACIFIC RIM CONFERENCE ON BIOTECHNOLOGY OF BACILLUS THURINGIENSIS AND ITS ENVIRONMENT IMPACT, 6., 2005, Victoria. Proceedings. Victoria: Biocontrol Network of Canada: Réseau Biocontrôle du Canada, 2007. p. 81-83.$c2007
650 $acorn
650 $aMilho
653 $aFall armyworm
653 $aInsect pathogen
653 $aSpodopera frugiperda
653 $aTransgenic maize
700 1 $aCARNEIRO, N. P.
700 1 $aUTIDA, R. H.
700 1 $aGUIMARAES, C. T.
700 1 $aMAGALHAES, J. V. de
700 1 $aPAIVA, E.
700 1 $aCARNEIRO, A. A.
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Embrapa Milho e Sorgo (CNPMS) |
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